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学科主题: Microbiology
题名: alpha-tocopherol is essential for acquired chill-light tolerance in the cyanobacterium Synechocystis sp strain PCC 6803
作者: Yang, Yang1; Yin, Chuntao1; Li, Weizhi1; Xu, Xudong1
通讯作者: Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
关键词: LIPID-PEROXIDATION ; VITAMIN-E ; HOMOGENTISATE PHYTYLTRANSFERASE ; MICROCYSTIS ; COLD ; ARABIDOPSIS ; PLANTS ; BIOSYNTHESIS ; EXPRESSION ; VECTORS
刊名: JOURNAL OF BACTERIOLOGY
发表日期: 2008-03-01
DOI: 10.1128/JB.01577-07
卷: 190, 期:5, 页:1554-1560
收录类别: SCI
文章类型: Article
部门归属: [Yang, Yang; Yin, Chuntao; Li, Weizhi; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: Unlike Escherichia coli, the cyanobacterium Synechocystis sp. strain PCC 6803 is insensitive to chill (5 degrees C) in the dark but rapidly losses viability when exposed to chill in the light (100 mu mol photons m(-2) s(-1)). Preconditioning at a low temperature (15 degrees C) greatly enhances the chill-light tolerance of Synechocystis sp. strain PCC 6803. This phenomenon is called acquired chill-light tolerance (ACLT). Preconditioned wild-type cells maintained a substantially higher level of alpha-tocopherol after exposure to chill-light stress. Mutants unable to synthesize alpha-tocopherol, such as slr1736, slr1737, slr0089, and slr0090 mutants, almost completely lost ACLT. When exposed to chill without light, these mutants showed no or a slight difference from the wild type. When complemented, the slr0089 mutant regained its ACLT. Copper-regulated expression of slr0090 from P-petE controlled the level of et-tocopherol and ACLT. We conclude that alpha-tocopherol is essential for ACLT of Synechocystis sp. strain PCC 6803. The role of a-tocopherol in ACLT may be based largely on a nonantioxidant activity that is not possessed by other tocopherols or pathway intermediates.
英文摘要: Unlike Escherichia coli, the cyanobacterium Synechocystis sp. strain PCC 6803 is insensitive to chill (5 degrees C) in the dark but rapidly losses viability when exposed to chill in the light (100 mu mol photons m(-2) s(-1)). Preconditioning at a low temperature (15 degrees C) greatly enhances the chill-light tolerance of Synechocystis sp. strain PCC 6803. This phenomenon is called acquired chill-light tolerance (ACLT). Preconditioned wild-type cells maintained a substantially higher level of alpha-tocopherol after exposure to chill-light stress. Mutants unable to synthesize alpha-tocopherol, such as slr1736, slr1737, slr0089, and slr0090 mutants, almost completely lost ACLT. When exposed to chill without light, these mutants showed no or a slight difference from the wild type. When complemented, the slr0089 mutant regained its ACLT. Copper-regulated expression of slr0090 from P-petE controlled the level of et-tocopherol and ACLT. We conclude that alpha-tocopherol is essential for ACLT of Synechocystis sp. strain PCC 6803. The role of a-tocopherol in ACLT may be based largely on a nonantioxidant activity that is not possessed by other tocopherols or pathway intermediates.
关键词[WOS]: LIPID-PEROXIDATION ; VITAMIN-E ; HOMOGENTISATE PHYTYLTRANSFERASE ; MICROCYSTIS ; COLD ; ARABIDOPSIS ; PLANTS ; BIOSYNTHESIS ; EXPRESSION ; VECTORS
语种: 英语
WOS记录号: WOS:000253554300008
ISSN号: 0021-9193
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8252
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Yang, Yang; Yin, Chuntao; Li, Weizhi; Xu, Xudong.alpha-tocopherol is essential for acquired chill-light tolerance in the cyanobacterium Synechocystis sp strain PCC 6803,JOURNAL OF BACTERIOLOGY,2008,190(5):1554-1560
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