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学科主题: Chemistry ; Analytical; Environmental Sciences
题名: A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples
作者: Hu, Chenlin1, 2; Gan, Nanqin1; He, Zhike3; Song, Lirong1
通讯作者: Song, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
关键词: microcystins ; gold nanoparticles ; chemiluminescence ; ELISA
刊名: INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
发表日期: 2008
DOI: 10.1080/03067310701657543
卷: 88, 期:4, 页:267-277
收录类别: SCI
文章类型: Article
部门归属: [Hu, Chenlin; Gan, Nanqin; Song, Lirong] CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Hu, Chenlin] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [He, Zhike] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ; Environmental Sciences
研究领域[WOS]: Chemistry ; Environmental Sciences & Ecology
摘要: A novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au3+-catalysed luminol chemiluminesent system. The method has a wide working range (0.05-10 mu g L-1, r(2) = 0.9914), the limit of detection was determined to be 0.024 mu g L-1, which is much lower than the World Health Organization's proposed guidelines (1 mu g L-1) for drinking-water. The proposed method was applied to MC analysis in natural water and fish tissue samples, and most results in the proposed method were in agreement with the conventional indirect competitive enzyme-linked immunosorbent assay method, which indicated that the new chemiluminescent immunoassay was sensitive, reliable, and suitable for MC analysis in natural water and fish tissue samples.
英文摘要: A novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au3+-catalysed luminol chemiluminesent system. The method has a wide working range (0.05-10 mu g L-1, r(2) = 0.9914), the limit of detection was determined to be 0.024 mu g L-1, which is much lower than the World Health Organization's proposed guidelines (1 mu g L-1) for drinking-water. The proposed method was applied to MC analysis in natural water and fish tissue samples, and most results in the proposed method were in agreement with the conventional indirect competitive enzyme-linked immunosorbent assay method, which indicated that the new chemiluminescent immunoassay was sensitive, reliable, and suitable for MC analysis in natural water and fish tissue samples.
关键词[WOS]: MASS-SPECTROMETRY ; WATER ; ANTIBODIES ; ALGAE ; ELISA ; QUANTIFICATION ; CYANOBACTERIA ; HEPATOTOXINS ; DEPOSITION ; NODULARIN
语种: 英语
WOS记录号: WOS:000253658600004
ISSN号: 0306-7319
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8244
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China

Recommended Citation:
Hu, Chenlin; Gan, Nanqin; He, Zhike; Song, Lirong.A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples,INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY,2008,88(4):267-277
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