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A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples
Hu, Chenlin1,2; Gan, Nanqin1; He, Zhike3; Song, Lirong1; Song, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
2008
Source PublicationINTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
ISSN0306-7319
Volume88Issue:4Pages:267-277
AbstractA novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au3+-catalysed luminol chemiluminesent system. The method has a wide working range (0.05-10 mu g L-1, r(2) = 0.9914), the limit of detection was determined to be 0.024 mu g L-1, which is much lower than the World Health Organization's proposed guidelines (1 mu g L-1) for drinking-water. The proposed method was applied to MC analysis in natural water and fish tissue samples, and most results in the proposed method were in agreement with the conventional indirect competitive enzyme-linked immunosorbent assay method, which indicated that the new chemiluminescent immunoassay was sensitive, reliable, and suitable for MC analysis in natural water and fish tissue samples.; A novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au3+-catalysed luminol chemiluminesent system. The method has a wide working range (0.05-10 mu g L-1, r(2) = 0.9914), the limit of detection was determined to be 0.024 mu g L-1, which is much lower than the World Health Organization's proposed guidelines (1 mu g L-1) for drinking-water. The proposed method was applied to MC analysis in natural water and fish tissue samples, and most results in the proposed method were in agreement with the conventional indirect competitive enzyme-linked immunosorbent assay method, which indicated that the new chemiluminescent immunoassay was sensitive, reliable, and suitable for MC analysis in natural water and fish tissue samples.
SubtypeArticle
KeywordMicrocystins Gold Nanoparticles Chemiluminescence Elisa
Department[Hu, Chenlin; Gan, Nanqin; Song, Lirong] CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Hu, Chenlin] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [He, Zhike] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
Subject AreaChemistry ; Analytical ; Environmental Sciences
DOI10.1080/03067310701657543
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Environmental Sciences & Ecology
WOS SubjectChemistry, Analytical ; Environmental Sciences
WOS IDWOS:000253658600004
WOS KeywordMASS-SPECTROMETRY ; WATER ; ANTIBODIES ; ALGAE ; ELISA ; QUANTIFICATION ; CYANOBACTERIA ; HEPATOTOXINS ; DEPOSITION ; NODULARIN
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Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8244
Collection期刊论文
Corresponding AuthorSong, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
Affiliation1.CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Hu, Chenlin,Gan, Nanqin,He, Zhike,et al. A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples[J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY,2008,88(4):267-277.
APA Hu, Chenlin,Gan, Nanqin,He, Zhike,Song, Lirong,&Song, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China.(2008).A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples.INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY,88(4),267-277.
MLA Hu, Chenlin,et al."A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples".INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY 88.4(2008):267-277.
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