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学科主题: Multidisciplinary Sciences
题名: Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes)
作者: Kong, Xianghui1, 2; Wang, Xuzhen1; Gan, Xiaoni1; Li, Junbing1; He, Shunping1
通讯作者: He, SP, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: connective tissue growth factor ; sequence variation ; molecular evolution ; Cyprinidae
刊名: PROGRESS IN NATURAL SCIENCE
发表日期: 2008-02-10
DOI: 10.1016/j.pnsc.2007.06.010
卷: 18, 期:2, 页:155-160
收录类别: SCI
文章类型: Article
部门归属: [Kong, Xianghui; Wang, Xuzhen; Gan, Xiaoni; Li, Junbing; He, Shunping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Kong, Xianghui] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China
WOS标题词: Science & Technology ; Technology
类目[WOS]: Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]: Materials Science ; Science & Technology - Other Topics
摘要: Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages, respectively, with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 by indel (insertion/deletion) was found at the 5' end of CTGF gene of Cyprinidae, and this 6 by deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要: Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages, respectively, with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 by indel (insertion/deletion) was found at the 5' end of CTGF gene of Cyprinidae, and this 6 by deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
关键词[WOS]: CYTOCHROME-B GENE ; PHYLOGENETIC-RELATIONSHIPS ; SEQUENCE ALIGNMENT ; FISHES ; ACTINOPTERYGII ; ACTIVATION ; INFERENCE ; SOFTWARE ; PACKAGE ; MRBAYES
语种: 英语
WOS记录号: WOS:000253767200004
ISSN号: 1002-0071
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8240
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China

Recommended Citation:
Kong, Xianghui; Wang, Xuzhen; Gan, Xiaoni; Li, Junbing; He, Shunping.Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes),PROGRESS IN NATURAL SCIENCE,2008,18(2):155-160
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