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Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes)
Kong, Xianghui1,2; Wang, Xuzhen1; Gan, Xiaoni1; Li, Junbing1; He, Shunping1; He, SP, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008-02-10
Source PublicationPROGRESS IN NATURAL SCIENCE
ISSN1002-0071
Volume18Issue:2Pages:155-160
AbstractConnective tissue growth factor (CTGF) plays an important role in regulation of cell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages, respectively, with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 by indel (insertion/deletion) was found at the 5' end of CTGF gene of Cyprinidae, and this 6 by deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.; Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages, respectively, with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 by indel (insertion/deletion) was found at the 5' end of CTGF gene of Cyprinidae, and this 6 by deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
SubtypeArticle
KeywordConnective Tissue Growth Factor Sequence Variation Molecular Evolution Cyprinidae
Department[Kong, Xianghui; Wang, Xuzhen; Gan, Xiaoni; Li, Junbing; He, Shunping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Kong, Xianghui] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China
Subject AreaMultidisciplinary Sciences
DOI10.1016/j.pnsc.2007.06.010
WOS HeadingsScience & Technology ; Technology
Indexed BySCI
Language英语
WOS Research AreaMaterials Science ; Science & Technology - Other Topics
WOS SubjectMaterials Science, Multidisciplinary ; Multidisciplinary Sciences
WOS IDWOS:000253767200004
WOS KeywordCYTOCHROME-B GENE ; PHYLOGENETIC-RELATIONSHIPS ; SEQUENCE ALIGNMENT ; FISHES ; ACTINOPTERYGII ; ACTIVATION ; INFERENCE ; SOFTWARE ; PACKAGE ; MRBAYES
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8240
Collection期刊论文
Corresponding AuthorHe, SP, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China
Recommended Citation
GB/T 7714
Kong, Xianghui,Wang, Xuzhen,Gan, Xiaoni,et al. Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes)[J]. PROGRESS IN NATURAL SCIENCE,2008,18(2):155-160.
APA Kong, Xianghui,Wang, Xuzhen,Gan, Xiaoni,Li, Junbing,He, Shunping,&He, SP, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes).PROGRESS IN NATURAL SCIENCE,18(2),155-160.
MLA Kong, Xianghui,et al."Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes)".PROGRESS IN NATURAL SCIENCE 18.2(2008):155-160.
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