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学科主题: Multidisciplinary Sciences
题名: Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803
作者: Yan, Chunlan1; Xu, Xudong1
通讯作者: Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: FBPase ; photoautotrophic growth ; light sensitivity ; Synechocystis sp.
刊名: PROGRESS IN NATURAL SCIENCE
发表日期: 2008-02-10
DOI: 10.1016/j.pnsc.2007.09.003
卷: 18, 期:2, 页:149-153
收录类别: SCI
文章类型: Article
部门归属: [Yan, Chunlan; Xu, Xudong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Technology
类目[WOS]: Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]: Materials Science ; Science & Technology - Other Topics
摘要: From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (rbpl), which encodes the fructose-1,6-biphosphatase (FBPase)/sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要: From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (rbpl), which encodes the fructose-1,6-biphosphatase (FBPase)/sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
关键词[WOS]: FRUCTOSE 1,6-BISPHOSPHATASE ; SYNECHOCOCCUS-LEOPOLIENSIS ; MOLECULAR CHARACTERIZATION ; HYDROGEN-PEROXIDE ; PHOTOSYSTEM-II ; PCC 6803 ; CHLOROPLAST ; FRUCTOSE-1,6-BISPHOSPHATASE ; PURIFICATION ; PROTEIN
语种: 英语
WOS记录号: WOS:000253767200003
ISSN号: 1002-0071
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8238
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Yan, Chunlan; Xu, Xudong.Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803,PROGRESS IN NATURAL SCIENCE,2008,18(2):149-153
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