| Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803 | |
| Yan, Chunlan; Xu, Xudong; Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China | |
| 2008-02-10 | |
| Source Publication | PROGRESS IN NATURAL SCIENCE
 |
| ISSN | 1002-0071 |
| Volume | 18Issue:2Pages:149-153 |
| Abstract | From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (rbpl), which encodes the fructose-1,6-biphosphatase (FBPase)/sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.; From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (rbpl), which encodes the fructose-1,6-biphosphatase (FBPase)/sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved. |
| Subtype | Article |
| Keyword | Fbpase Photoautotrophic Growth Light Sensitivity Synechocystis Sp. |
| Department | [Yan, Chunlan; Xu, Xudong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Subject Area | Multidisciplinary Sciences |
| DOI | 10.1016/j.pnsc.2007.09.003 |
| WOS Headings | Science & Technology ; Technology |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Materials Science ; Science & Technology - Other Topics |
| WOS Subject | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| WOS ID | WOS:000253767200003 |
| WOS Keyword | FRUCTOSE 1,6-BISPHOSPHATASE ; SYNECHOCOCCUS-LEOPOLIENSIS ; MOLECULAR CHARACTERIZATION ; HYDROGEN-PEROXIDE ; PHOTOSYSTEM-II ; PCC 6803 ; CHLOROPLAST ; FRUCTOSE-1,6-BISPHOSPHATASE ; PURIFICATION ; PROTEIN |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8238 |
| Collection | 期刊论文 |
| Corresponding Author | Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Yan, Chunlan,Xu, Xudong,Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803[J]. PROGRESS IN NATURAL SCIENCE,2008,18(2):149-153. |
| APA | Yan, Chunlan,Xu, Xudong,&Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803.PROGRESS IN NATURAL SCIENCE,18(2),149-153. |
| MLA | Yan, Chunlan,et al."Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803".PROGRESS IN NATURAL SCIENCE 18.2(2008):149-153. |
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