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The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR
Huang, Wen Min1,2; Xing, Wei3; Li, Dun Hai1; Liu, Yong Ding1; Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-03-01
Source PublicationBULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
ISSN0007-4861
Volume80Issue:3Pages:215-219
AbstractThis study was undertaken to investigate the role of the glutathione-involved detoxifying mechanism in defending the tobacco BY-2 suspension cells against microcystin-RR (MC-RR). Analysis showed that exposure of the cells to different concentrations of MC-RR (0.1, 1 and 10 mu g/mL) for 0-6 days resulted in a time and concentration-dependent decrease in cell viability and increase in reactive oxygen species (ROS) content. Reduced glutathione (GSH) and total glutathione (tGSH) content as well as glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) activities significantly increased after 3-4 days exposure in the highest two concentration treated groups, while decreased until reaching the control values except for GPX at day 6. Oxidized glutathione (GSSG) content markedly increased compared with control in high concentration MC-RR treated group after 6 days exposure. The GSH/GSSG ratio was much higher than control in 10 mu g/mL MC-RR treated group at day 4, but after 6 days exposure, the ratios in all treated groups were lower than that of the control group.; This study was undertaken to investigate the role of the glutathione-involved detoxifying mechanism in defending the tobacco BY-2 suspension cells against microcystin-RR (MC-RR). Analysis showed that exposure of the cells to different concentrations of MC-RR (0.1, 1 and 10 mu g/mL) for 0-6 days resulted in a time and concentration-dependent decrease in cell viability and increase in reactive oxygen species (ROS) content. Reduced glutathione (GSH) and total glutathione (tGSH) content as well as glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) activities significantly increased after 3-4 days exposure in the highest two concentration treated groups, while decreased until reaching the control values except for GPX at day 6. Oxidized glutathione (GSSG) content markedly increased compared with control in high concentration MC-RR treated group after 6 days exposure. The GSH/GSSG ratio was much higher than control in 10 mu g/mL MC-RR treated group at day 4, but after 6 days exposure, the ratios in all treated groups were lower than that of the control group.
SubtypeArticle
KeywordMicrocystin-rr Tobacco By-2 Suspension Cells Glutathione Metabolism Oxidative Stress
Department[Huang, Wen Min; Li, Dun Hai; Liu, Yong Ding] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Huang, Wen Min] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Xing, Wei] Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
Subject AreaEnvironmental Sciences ; Toxicology
DOI10.1007/s00128-007-9348-7
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000253993500008
WOS KeywordOXIDATIVE STRESS ; LR ; DEFENSE ; SYSTEM
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8226
Collection期刊论文
Corresponding AuthorLiu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
Recommended Citation
GB/T 7714
Huang, Wen Min,Xing, Wei,Li, Dun Hai,et al. The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR[J]. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2008,80(3):215-219.
APA Huang, Wen Min,Xing, Wei,Li, Dun Hai,Liu, Yong Ding,&Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR.BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,80(3),215-219.
MLA Huang, Wen Min,et al."The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR".BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 80.3(2008):215-219.
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