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学科主题: Environmental Sciences; Toxicology
题名: The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR
作者: Huang, Wen Min1, 2; Xing, Wei3; Li, Dun Hai1; Liu, Yong Ding1
通讯作者: Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Microcystin-RR ; tobacco BY-2 suspension cells ; glutathione metabolism ; oxidative stress
刊名: BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
发表日期: 2008-03-01
DOI: 10.1007/s00128-007-9348-7
卷: 80, 期:3, 页:215-219
收录类别: SCI
文章类型: Article
部门归属: [Huang, Wen Min; Li, Dun Hai; Liu, Yong Ding] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Huang, Wen Min] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Xing, Wei] Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences ; Toxicology
研究领域[WOS]: Environmental Sciences & Ecology ; Toxicology
摘要: This study was undertaken to investigate the role of the glutathione-involved detoxifying mechanism in defending the tobacco BY-2 suspension cells against microcystin-RR (MC-RR). Analysis showed that exposure of the cells to different concentrations of MC-RR (0.1, 1 and 10 mu g/mL) for 0-6 days resulted in a time and concentration-dependent decrease in cell viability and increase in reactive oxygen species (ROS) content. Reduced glutathione (GSH) and total glutathione (tGSH) content as well as glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) activities significantly increased after 3-4 days exposure in the highest two concentration treated groups, while decreased until reaching the control values except for GPX at day 6. Oxidized glutathione (GSSG) content markedly increased compared with control in high concentration MC-RR treated group after 6 days exposure. The GSH/GSSG ratio was much higher than control in 10 mu g/mL MC-RR treated group at day 4, but after 6 days exposure, the ratios in all treated groups were lower than that of the control group.
英文摘要: This study was undertaken to investigate the role of the glutathione-involved detoxifying mechanism in defending the tobacco BY-2 suspension cells against microcystin-RR (MC-RR). Analysis showed that exposure of the cells to different concentrations of MC-RR (0.1, 1 and 10 mu g/mL) for 0-6 days resulted in a time and concentration-dependent decrease in cell viability and increase in reactive oxygen species (ROS) content. Reduced glutathione (GSH) and total glutathione (tGSH) content as well as glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) activities significantly increased after 3-4 days exposure in the highest two concentration treated groups, while decreased until reaching the control values except for GPX at day 6. Oxidized glutathione (GSSG) content markedly increased compared with control in high concentration MC-RR treated group after 6 days exposure. The GSH/GSSG ratio was much higher than control in 10 mu g/mL MC-RR treated group at day 4, but after 6 days exposure, the ratios in all treated groups were lower than that of the control group.
关键词[WOS]: OXIDATIVE STRESS ; LR ; DEFENSE ; SYSTEM
语种: 英语
WOS记录号: WOS:000253993500008
ISSN号: 0007-4861
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8226
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China

Recommended Citation:
Huang, Wen Min; Xing, Wei; Li, Dun Hai; Liu, Yong Ding.The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR,BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2008,80(3):215-219
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