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Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Flavobacterium columnare
Liu, G. Y.1,2; Nie, P.1,2; Zhang, J.1,2; Li, N.1,2; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2008-04-01
Source PublicationJOURNAL OF FISH DISEASES
ISSN0140-7775
Volume31Issue:4Pages:269-276
AbstractOuter membrane proteins (OMPs) of bacteria are key molecules interacting with the host environment. Flavobacterium columnare, a pathogen-causing columnaris disease of fish worldwide, was studied in order to understand the composition of its OMPs. The sarcosine-insoluble membrane fraction of the OMPs was analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in combination with reverse-phase high-performance liquid chromatography-tandem mass spectrometry (RP-HPLC MS/MS). Thirty-six proteins were identified, including proteins involved in cell wall/membrane biogenesis, specific transport of various nutrients and in essential metabolism. The present study is the first report on the OMPs of F. columnare, and may serve as the basis for understanding the pathogenesis of the bacterium.; Outer membrane proteins (OMPs) of bacteria are key molecules interacting with the host environment. Flavobacterium columnare, a pathogen-causing columnaris disease of fish worldwide, was studied in order to understand the composition of its OMPs. The sarcosine-insoluble membrane fraction of the OMPs was analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in combination with reverse-phase high-performance liquid chromatography-tandem mass spectrometry (RP-HPLC MS/MS). Thirty-six proteins were identified, including proteins involved in cell wall/membrane biogenesis, specific transport of various nutrients and in essential metabolism. The present study is the first report on the OMPs of F. columnare, and may serve as the basis for understanding the pathogenesis of the bacterium.
SubtypeArticle
KeywordFlavobacterium Columnare Outer Membrane Protein Proteome Rp-hplc Ms/ms
Department[Liu, G. Y.; Nie, P.; Zhang, J.; Li, N.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Liu, G. Y.; Nie, P.; Zhang, J.; Li, N.] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1111/j.1365-2761.2007.00898.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000254126900004
WOS Keyword2-DIMENSIONAL ELECTROPHORESIS ; PORPHYROMONAS-GINGIVALIS ; FLEXIBACTER-COLUMNARIS ; ESCHERICHIA-COLI ; PROTEINS ; IDENTIFICATION ; SEQUENCE ; FISH
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8220
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Liu, G. Y.,Nie, P.,Zhang, J.,et al. Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Flavobacterium columnare[J]. JOURNAL OF FISH DISEASES,2008,31(4):269-276.
APA Liu, G. Y.,Nie, P.,Zhang, J.,Li, N.,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.(2008).Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Flavobacterium columnare.JOURNAL OF FISH DISEASES,31(4),269-276.
MLA Liu, G. Y.,et al."Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Flavobacterium columnare".JOURNAL OF FISH DISEASES 31.4(2008):269-276.
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