IHB OpenIR  > 期刊论文
Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF
Tao, Jian-Jun; Zhou, Guang-Zhou; Gui, Han-Farig; Zhang, Qi-Ya; Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-03-01
Source PublicationVIRUS RESEARCH
ISSN0168-1702
Volume132Issue:1-2Pages:86-96
AbstractThe complete genome of mandarin fish Siniperca chuatsi rhabdovirus (SCRV) was cloned and sequenced. It comprises 11,545 nucleotides and contains five genes encoding the nucleoprotein N, the phosphoprotein P, the matrix protein M, the glycoprotein G, and the RNA-dependent RNA polymerase protein L. At the 3' and 5' termini of SCRV genome, leader and trailer sequences show inverse complementarity. The N, P, M and G proteins share the highest sequence identities (ranging from 14.8 to 41.5%) with the respective proteins of rhabdovirus 903/87, the L protein has the highest identity with those of vesiculoviruses, especially with Chandipura virus (44.7%). Phylogenetic analysis of L proteins showed that SCRV clustered with spring vireamia of carp virus (SVCV) and was most closely related to viruses in the genus Vesiculovirus. In addition, an overlapping open reading frame (ORF) predicted to encode a protein similar to vesicular stomatitis virus C protein is present within the P gene of SCRV. Furthermore, an unoverlapping small ORF downstream of M ORF within M gene is predicted (tentatively called orf4). Therefore, the genomic organization of SCRV can be proposed as 3' leader-N-P/C-M-(orf4)-G-L-trailer 5'. Orf4 transcription or translation products could not be detected by northern or Western blot, respectively, though one similar mRNA band to M mRNA was found. This is the first report on one small unoverlapping ORF in M gene of a fish rhabdovirus. (c) 2007 Elsevier B.V. All rights reserved.; The complete genome of mandarin fish Siniperca chuatsi rhabdovirus (SCRV) was cloned and sequenced. It comprises 11,545 nucleotides and contains five genes encoding the nucleoprotein N, the phosphoprotein P, the matrix protein M, the glycoprotein G, and the RNA-dependent RNA polymerase protein L. At the 3' and 5' termini of SCRV genome, leader and trailer sequences show inverse complementarity. The N, P, M and G proteins share the highest sequence identities (ranging from 14.8 to 41.5%) with the respective proteins of rhabdovirus 903/87, the L protein has the highest identity with those of vesiculoviruses, especially with Chandipura virus (44.7%). Phylogenetic analysis of L proteins showed that SCRV clustered with spring vireamia of carp virus (SVCV) and was most closely related to viruses in the genus Vesiculovirus. In addition, an overlapping open reading frame (ORF) predicted to encode a protein similar to vesicular stomatitis virus C protein is present within the P gene of SCRV. Furthermore, an unoverlapping small ORF downstream of M ORF within M gene is predicted (tentatively called orf4). Therefore, the genomic organization of SCRV can be proposed as 3' leader-N-P/C-M-(orf4)-G-L-trailer 5'. Orf4 transcription or translation products could not be detected by northern or Western blot, respectively, though one similar mRNA band to M mRNA was found. This is the first report on one small unoverlapping ORF in M gene of a fish rhabdovirus. (c) 2007 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordGenomic Sequence Rhabdovirus Mandarin Fish Non-transcriptional Orf
Department[Tao, Jian-Jun; Zhou, Guang-Zhou; Gui, Han-Farig; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaVirology
DOI10.1016/j.virusres.2007.10.018
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000254109100010
WOS KeywordVESICULAR STOMATITIS-VIRUS ; HEMATOPOIETIC NECROSIS VIRUS ; COMPLETE NUCLEOTIDE-SEQUENCE ; HEMORRHAGIC SEPTICEMIA VIRUS ; EPHEMERAL FEVER RHABDOVIRUS ; RNA-POLYMERASE ; MOLECULAR CHARACTERIZATION ; GENE JUNCTIONS ; VIRAL-RNA ; N PROTEIN
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8216
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Tao, Jian-Jun,Zhou, Guang-Zhou,Gui, Han-Farig,et al. Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF[J]. VIRUS RESEARCH,2008,132(1-2):86-96.
APA Tao, Jian-Jun,Zhou, Guang-Zhou,Gui, Han-Farig,Zhang, Qi-Ya,&Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF.VIRUS RESEARCH,132(1-2),86-96.
MLA Tao, Jian-Jun,et al."Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF".VIRUS RESEARCH 132.1-2(2008):86-96.
Files in This Item:
File Name/Size DocType Version Access License
Genomic sequence of (936KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tao, Jian-Jun]'s Articles
[Zhou, Guang-Zhou]'s Articles
[Gui, Han-Farig]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tao, Jian-Jun]'s Articles
[Zhou, Guang-Zhou]'s Articles
[Gui, Han-Farig]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tao, Jian-Jun]'s Articles
[Zhou, Guang-Zhou]'s Articles
[Gui, Han-Farig]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.