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学科主题: Environmental Sciences; Toxicology; Water Resources
题名: Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus
作者: Sun, Yuhua1; Tang, Rong1; Li, Dapeng1; Zhang, Xuzhen1; Fu, Juan2; Xie, Ping1, 2
通讯作者: Xie, P, Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China
关键词: microcystins ; mRNA expression ; antioxidant enzymes ; crucian carp (Carassius auratus)
刊名: ENVIRONMENTAL TOXICOLOGY
发表日期: 2008-04-01
DOI: 10.1002/tox.20317
卷: 23, 期:2, 页:145-152
收录类别: SCI
文章类型: Article
部门归属: [Sun, Yuhua; Tang, Rong; Li, Dapeng; Zhang, Xuzhen; Xie, Ping] Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China; [Fu, Juan; Xie, Ping] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Environmental Sciences ; Toxicology ; Water Resources
研究领域[WOS]: Environmental Sciences & Ecology ; Toxicology ; Water Resources
摘要: Recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of MCs toxicity. In the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mu g MC-LReq per kg body weight (BW). We reported the cDNA sequences for four kinds of antioxidant enzyme (GSH-PX, CAT, Cu/Zn SOD, and GR) genes, and evaluated the oxidant stress induced by MCs through analyzing the transcription abundance of antioxidant enzyme genes using real-time PCR method. The time-dependent change of relative transcription abundance and expression of the antioxiclant enzyme genes were determined at 1, 3, 12, 24, and 48 h. The transcription abundance varied among antioxiclant enzymes, with GSH-PX and GR down-regulation, and CAT and SOD significantly upregulation. Based on these data, we tentatively concluded that the oxidant stress was induced by MCs, and caused the different response of the antioxiclant enzyme genes. (c) 2008 Wiley Periodicals, Inc.
英文摘要: Recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of MCs toxicity. In the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mu g MC-LReq per kg body weight (BW). We reported the cDNA sequences for four kinds of antioxidant enzyme (GSH-PX, CAT, Cu/Zn SOD, and GR) genes, and evaluated the oxidant stress induced by MCs through analyzing the transcription abundance of antioxidant enzyme genes using real-time PCR method. The time-dependent change of relative transcription abundance and expression of the antioxiclant enzyme genes were determined at 1, 3, 12, 24, and 48 h. The transcription abundance varied among antioxiclant enzymes, with GSH-PX and GR down-regulation, and CAT and SOD significantly upregulation. Based on these data, we tentatively concluded that the oxidant stress was induced by MCs, and caused the different response of the antioxiclant enzyme genes. (c) 2008 Wiley Periodicals, Inc.
关键词[WOS]: FISH OREOCHROMIS SP. ; INDUCED DNA-DAMAGE ; OXIDATIVE STRESS ; IN-VIVO ; LIPID-PEROXIDATION ; DANIO-RERIO ; LR ; LIVER ; CYANOBACTERIA ; HEPATOCYTES
语种: 英语
WOS记录号: WOS:000254495400001
ISSN号: 1520-4081
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8214
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Sun, Yuhua; Tang, Rong; Li, Dapeng; Zhang, Xuzhen; Fu, Juan; Xie, Ping.Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus,ENVIRONMENTAL TOXICOLOGY,2008,23(2):145-152
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