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Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus
Sun, Yuhua1; Tang, Rong1; Li, Dapeng1; Zhang, Xuzhen1; Fu, Juan2; Xie, Ping1,2; Xie, P, Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China
2008-04-01
Source PublicationENVIRONMENTAL TOXICOLOGY
ISSN1520-4081
Volume23Issue:2Pages:145-152
AbstractRecent evidences suggested that oxidative stress may play a significant role in the pathogenesis of MCs toxicity. In the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mu g MC-LReq per kg body weight (BW). We reported the cDNA sequences for four kinds of antioxidant enzyme (GSH-PX, CAT, Cu/Zn SOD, and GR) genes, and evaluated the oxidant stress induced by MCs through analyzing the transcription abundance of antioxidant enzyme genes using real-time PCR method. The time-dependent change of relative transcription abundance and expression of the antioxiclant enzyme genes were determined at 1, 3, 12, 24, and 48 h. The transcription abundance varied among antioxiclant enzymes, with GSH-PX and GR down-regulation, and CAT and SOD significantly upregulation. Based on these data, we tentatively concluded that the oxidant stress was induced by MCs, and caused the different response of the antioxiclant enzyme genes. (c) 2008 Wiley Periodicals, Inc.; Recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of MCs toxicity. In the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mu g MC-LReq per kg body weight (BW). We reported the cDNA sequences for four kinds of antioxidant enzyme (GSH-PX, CAT, Cu/Zn SOD, and GR) genes, and evaluated the oxidant stress induced by MCs through analyzing the transcription abundance of antioxidant enzyme genes using real-time PCR method. The time-dependent change of relative transcription abundance and expression of the antioxiclant enzyme genes were determined at 1, 3, 12, 24, and 48 h. The transcription abundance varied among antioxiclant enzymes, with GSH-PX and GR down-regulation, and CAT and SOD significantly upregulation. Based on these data, we tentatively concluded that the oxidant stress was induced by MCs, and caused the different response of the antioxiclant enzyme genes. (c) 2008 Wiley Periodicals, Inc.
SubtypeArticle
KeywordMicrocystins Mrna Expression Antioxidant Enzymes Crucian Carp (Carassius Auratus)
Department[Sun, Yuhua; Tang, Rong; Li, Dapeng; Zhang, Xuzhen; Xie, Ping] Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China; [Fu, Juan; Xie, Ping] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaEnvironmental Sciences ; Toxicology ; Water Resources
DOI10.1002/tox.20317
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology ; Water Resources
WOS SubjectEnvironmental Sciences ; Toxicology ; Water Resources
WOS IDWOS:000254495400001
WOS KeywordFISH OREOCHROMIS SP. ; INDUCED DNA-DAMAGE ; OXIDATIVE STRESS ; IN-VIVO ; LIPID-PEROXIDATION ; DANIO-RERIO ; LR ; LIVER ; CYANOBACTERIA ; HEPATOCYTES
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8214
Collection期刊论文
Corresponding AuthorXie, P, Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China
Affiliation1.Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Sun, Yuhua,Tang, Rong,Li, Dapeng,et al. Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus[J]. ENVIRONMENTAL TOXICOLOGY,2008,23(2):145-152.
APA Sun, Yuhua.,Tang, Rong.,Li, Dapeng.,Zhang, Xuzhen.,Fu, Juan.,...&Xie, P, Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China.(2008).Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus.ENVIRONMENTAL TOXICOLOGY,23(2),145-152.
MLA Sun, Yuhua,et al."Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus".ENVIRONMENTAL TOXICOLOGY 23.2(2008):145-152.
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