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Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos
Pei, De-Sheng1,2; Sun, Yong-Hua1; Chen, Chun-Hong1,3; Chen, Shang-Ping1; Wang, Ya-Ping1; Hu, Wei1; Zhu, Zuo-Yan1; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-03-18
Source PublicationBMC DEVELOPMENTAL BIOLOGY
ISSN1471-213X
Volume8Pages:-
Abstractm Background: Cross-species nuclear transfer has been shown to be a potent approach to retain the genetic viability of a certain species near extinction. However, most embryos produced by cross-species nuclear transfer were compromised because that they were unable to develop to later stages. Gene expression analysis of cross-species cloned embryos will yield new insights into the regulatory mechanisms involved in cross-species nuclear transfer and embryonic development. Results: A novel gene, K31, was identified as an up-regulated gene in fish cross-subfamily cloned embryos using SSH approach and RACE method. K31 complete cDNA sequence is 1106 base pairs (bp) in length, with a 342 bp open reading frame (ORF) encoding a putative protein of 113 amino acids (aa). Comparative analysis revealed no homologous known gene in zebrafish and other species database. K31 protein contains a putative transmembrane helix and five putative phosphorylation sites but without a signal peptide. Expression pattern analysis by real time RT-PCR and whole-mount in situ hybridization (WISH) shows that it has the characteristics of constitutively expressed gene. Sub-cellular localization assay shows that K31 protein can not penetrate the nuclei. Interestingly, over-expression of K31 gene can cause lethality in the epithelioma papulosum cyprinid (EPC) cells in cell culture, which gave hint to the inefficient reprogramming events occurred in cloned embryos. Conclusion: Taken together, our findings indicated that K31 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos and over-expression of K31 gene can cause lethality of cultured fish cells. To our knowledge, this is the first report on the determination of novel genes involved in nucleo-cytoplasmic interaction of fish cross-subfamily cloned embryos.; m Background: Cross-species nuclear transfer has been shown to be a potent approach to retain the genetic viability of a certain species near extinction. However, most embryos produced by cross-species nuclear transfer were compromised because that they were unable to develop to later stages. Gene expression analysis of cross-species cloned embryos will yield new insights into the regulatory mechanisms involved in cross-species nuclear transfer and embryonic development.
SubtypeArticle
KeywordNuclear Transfer Gobiocypris-rarus Reactivation Prediction Cloning Signal Fish
Department[Pei, De-Sheng; Sun, Yong-Hua; Chen, Chun-Hong; Chen, Shang-Ping; Wang, Ya-Ping; Hu, Wei; Zhu, Zuo-Yan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Pei, De-Sheng] Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China; [Chen, Chun-Hong] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Subject AreaDevelopmental Biology
DOI10.1186/1471-213X-8-29
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000254616600001
WOS KeywordNUCLEAR TRANSFER ; GOBIOCYPRIS-RARUS ; REACTIVATION ; PREDICTION ; CLONING ; SIGNAL ; FISH
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8198
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Pei, De-Sheng,Sun, Yong-Hua,Chen, Chun-Hong,et al. Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos[J]. BMC DEVELOPMENTAL BIOLOGY,2008,8:-.
APA Pei, De-Sheng.,Sun, Yong-Hua.,Chen, Chun-Hong.,Chen, Shang-Ping.,Wang, Ya-Ping.,...&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos.BMC DEVELOPMENTAL BIOLOGY,8,-.
MLA Pei, De-Sheng,et al."Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos".BMC DEVELOPMENTAL BIOLOGY 8(2008):-.
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