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The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8
Harun, Nor Omaima1; Zou, Jun1; Zhang, Yang-An1,2; Nie, Pin2; Secombes, Chris J.1; Secombes, CJ, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Tillydrone Ave, Aberdeen AB24 2TZ, Scotland
2008
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume32Issue:6Pages:673-681
AbstractIn this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved.; In this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordRainbow Trout Recombinant Protein Interleukin-8 Biological Activity Migration Respiratory Burst
Department[Harun, Nor Omaima; Zou, Jun; Zhang, Yang-An; Secombes, Chris J.] Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland; [Zhang, Yang-An; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaImmunology ; Zoology
DOI10.1016/j.dci.2007.10.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000254781600009
WOS KeywordPERITONEAL-CAVITY ; FUNCTIONAL-CHARACTERIZATION ; HOST DEFENSE ; IN-VITRO ; NEUTROPHIL ; CLONING ; CHEMOTAXIS ; LEUKOCYTES ; MIGRATION ; CYTOKINE
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8186
Collection期刊论文
Corresponding AuthorSecombes, CJ, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Tillydrone Ave, Aberdeen AB24 2TZ, Scotland
Affiliation1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Harun, Nor Omaima,Zou, Jun,Zhang, Yang-An,et al. The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2008,32(6):673-681.
APA Harun, Nor Omaima,Zou, Jun,Zhang, Yang-An,Nie, Pin,Secombes, Chris J.,&Secombes, CJ, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Tillydrone Ave, Aberdeen AB24 2TZ, Scotland.(2008).The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,32(6),673-681.
MLA Harun, Nor Omaima,et al."The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 32.6(2008):673-681.
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