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Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus
Zhou, Guang-Zhou; Gui, Lang; Li, Zheng-Qiu; Yuan, Xiu-Ping; Zhang, Qi-Ya; Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-03-01
Source PublicationJOURNAL OF VIROLOGICAL METHODS
ISSN0166-0934
Volume148Issue:1-2Pages:205-210
AbstractTwo MAbs (3C7 and 3C9) against flounder Paralichthys olivaceus rhabdovirus (PORV) were generated with hybridoma cell fusion technology and characterized by an indirect enzyme-linked immunosorbent assay, isotype test, Western blot and immunodot analysis and immunofluorescence assay. Isotyping tests demonstrated that both of the two MAbs belonged to IgM subclass. Western blot analysis showed the MAbs reacted with 42, 30, and 22 kDa viral proteins, which were localized within the cytoplasm of PORV-infected grass carp ovary (GCO) cells analyzed by indirect immunofluorescences tests. The MAb 3C7 was also selected at random for detecting virus antigens in the inoculated grass carp tissues by immunohistochemistry assay. Flow cytometry tests showed that at the 36 h postinfection (0.25 PFU/cell), the 23% PORV-infected GCO cells could be distinguished from the uninfected cells with the MAb 3C7. Such MAbs could be useful for diagnosis and potential treatment of viral infection. (C) 2007 Elsevier B.V. All rights reserved.; Two MAbs (3C7 and 3C9) against flounder Paralichthys olivaceus rhabdovirus (PORV) were generated with hybridoma cell fusion technology and characterized by an indirect enzyme-linked immunosorbent assay, isotype test, Western blot and immunodot analysis and immunofluorescence assay. Isotyping tests demonstrated that both of the two MAbs belonged to IgM subclass. Western blot analysis showed the MAbs reacted with 42, 30, and 22 kDa viral proteins, which were localized within the cytoplasm of PORV-infected grass carp ovary (GCO) cells analyzed by indirect immunofluorescences tests. The MAb 3C7 was also selected at random for detecting virus antigens in the inoculated grass carp tissues by immunohistochemistry assay. Flow cytometry tests showed that at the 36 h postinfection (0.25 PFU/cell), the 23% PORV-infected GCO cells could be distinguished from the uninfected cells with the MAb 3C7. Such MAbs could be useful for diagnosis and potential treatment of viral infection. (C) 2007 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordFish Rhabdoviruses Monoclonal Antibodies Immunocytochemistry Flow Cytometry
Department[Zhou, Guang-Zhou; Gui, Lang; Li, Zheng-Qiu; Yuan, Xiu-Ping; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
DOI10.1016/j.jviromet.2007.11.010
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
WOS IDWOS:000254720500026
WOS KeywordVIRAL HEMORRHAGIC SEPTICEMIA ; SCOPHTHALMUS-MAXIMUS RHABDOVIRUS ; LYMPHOCYSTIS DISEASE VIRUS ; COMPLETE GENOMIC SEQUENCE ; FISH RHABDOVIRUS ; SPRING VIREMIA ; CARP VIRUS ; RABIES ; ASSAY ; DIAGNOSIS
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8178
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Guang-Zhou,Gui, Lang,Li, Zheng-Qiu,et al. Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus[J]. JOURNAL OF VIROLOGICAL METHODS,2008,148(1-2):205-210.
APA Zhou, Guang-Zhou,Gui, Lang,Li, Zheng-Qiu,Yuan, Xiu-Ping,Zhang, Qi-Ya,&Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus.JOURNAL OF VIROLOGICAL METHODS,148(1-2),205-210.
MLA Zhou, Guang-Zhou,et al."Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus".JOURNAL OF VIROLOGICAL METHODS 148.1-2(2008):205-210.
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