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Distribution of toxins in various tissues of crucian carp intraperitoneally injected with hepatotoxic microcystins
Lei, Hehua1; Xie, Ping1; Chen, Jun1; Liang, Gaodao1; Dai, Ming1; Zhang, Xuezhen2; Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
2008-05-01
Source PublicationENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
ISSN0730-7268
Volume27Issue:5Pages:1167-1174
AbstractAn acute toxicity experiment was conducted to examine the distribution and depuration of microcystins (MCS) in crucian carp (Carassius aurutus) tissues. Fish were injected intraperitoneally with extracted MCs at a dose of 200 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight. Microcystin concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, and 48 h postinjection using liquid chromatography coupled with mass spectrometry. Microcystins were detected mainly in blood (3.99% of injected dose at 1 h), liver (1.60% at I h), gonad (1.49% at 3 h), and kidney (0.14% at 48 h). Other tissues, such as the heart, gill, gallbladder, intestine, spleen, brain, and muscle, contained less than 0.1% of the injected MCs. The highest concentration of MCs was found in blood (526-3,753 ng/g dry wt), followed by liver (103-1,656 ng/g dry wt) and kidney (279-1,592 ng/g dry wt). No MC-LR was detectable in intestine, spleen, kidney, brain, and muscle, whereas MC-RR was found in all examined fish tissues, which might result from organ specificity of different MCs. Clearance of MC-RR in brain tissue was slow. In kidney, the MC-RR content was negatively correlated with that in blood, suggesting that blood was important in the transportation of MC-RR to kidney for excretion.; An acute toxicity experiment was conducted to examine the distribution and depuration of microcystins (MCS) in crucian carp (Carassius aurutus) tissues. Fish were injected intraperitoneally with extracted MCs at a dose of 200 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight. Microcystin concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, and 48 h postinjection using liquid chromatography coupled with mass spectrometry. Microcystins were detected mainly in blood (3.99% of injected dose at 1 h), liver (1.60% at I h), gonad (1.49% at 3 h), and kidney (0.14% at 48 h). Other tissues, such as the heart, gill, gallbladder, intestine, spleen, brain, and muscle, contained less than 0.1% of the injected MCs. The highest concentration of MCs was found in blood (526-3,753 ng/g dry wt), followed by liver (103-1,656 ng/g dry wt) and kidney (279-1,592 ng/g dry wt). No MC-LR was detectable in intestine, spleen, kidney, brain, and muscle, whereas MC-RR was found in all examined fish tissues, which might result from organ specificity of different MCs. Clearance of MC-RR in brain tissue was slow. In kidney, the MC-RR content was negatively correlated with that in blood, suggesting that blood was important in the transportation of MC-RR to kidney for excretion.
SubtypeArticle
KeywordMicrocystins Intraperitoneal Injection Crucian Carp Tissue Distribution Depuration
Department[Lei, Hehua; Xie, Ping; Chen, Jun; Liang, Gaodao; Dai, Ming] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China; [Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China
Subject AreaEnvironmental Sciences ; Toxicology
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000255063700021
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; PHYTOPLANKTIVOROUS SILVER CARP ; FRESH-WATER SNAIL ; RAINBOW-TROUT ; EUTROPHIC LAKE ; DANIO-RERIO ; BIOCHEMICAL-CHARACTERIZATION ; CORYDORAS-PALEATUS ; SEASONAL DYNAMICS ; SUBTROPICAL CHINA
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Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8172
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Lei, Hehua,Xie, Ping,Chen, Jun,et al. Distribution of toxins in various tissues of crucian carp intraperitoneally injected with hepatotoxic microcystins[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2008,27(5):1167-1174.
APA Lei, Hehua.,Xie, Ping.,Chen, Jun.,Liang, Gaodao.,Dai, Ming.,...&Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China.(2008).Distribution of toxins in various tissues of crucian carp intraperitoneally injected with hepatotoxic microcystins.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,27(5),1167-1174.
MLA Lei, Hehua,et al."Distribution of toxins in various tissues of crucian carp intraperitoneally injected with hepatotoxic microcystins".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 27.5(2008):1167-1174.
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