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Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection
Su, Jianguo1,2; Zhu, Zuoyan1; Wang, Yaping1; Zou, Jun3; Hu, Wei1; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008-04-01
Source PublicationIMMUNOGENETICS
ISSN0093-7711
Volume60Issue:3-4Pages:195-205
AbstractToll-like receptor 3 (TLR3) plays a key role in activating immune responses during viral infection. To study the genes involved in the regulatory function of TLR3 in the rare minnow Gobiocypris rarus after viral infection, a full-length cDNA of TLR3 (GrTLR3) with a splice variant (GrTLR3s) was identified by homologous cloning and RACE techniques. The antiviral effector molecule Mx gene was cloned and partially sequenced. The mRNA expression levels of GrTLR3, GrTLR3s, and Mx were studied in different tissues before and after virus infection by real-time quantitative RT-PCR. The transcripts of all three genes in liver were significantly increased following GCRV infection (P<0.05). The mRNA levels in liver were upregulated at 24 h post-injection for GrTLR3 and GrTLR3s, and at 12 h for Mx. The upregulated expression levels were several folds for GrTLR3s, tens of folds for GrTLR3, and hundreds of folds for Mx. By semi-quantitative RT-PCR, GrTLR3 and Mx expressed at all the developmental stages, whereas GrTLR3s could only be detected at later developmental stages. Using RNAi and transgenic techniques, GrTLR3 mediated Mx expression but GrTLR3s did not. The time-dependent upregulation of receptor and effector, and the Mx over-expression dependent on TLR3, indicated that GrTLR3 regulated Mx expression in viral infection through a configuration change in rare minnow, and its splice variant did not contribute to the process.; Toll-like receptor 3 (TLR3) plays a key role in activating immune responses during viral infection. To study the genes involved in the regulatory function of TLR3 in the rare minnow Gobiocypris rarus after viral infection, a full-length cDNA of TLR3 (GrTLR3) with a splice variant (GrTLR3s) was identified by homologous cloning and RACE techniques. The antiviral effector molecule Mx gene was cloned and partially sequenced. The mRNA expression levels of GrTLR3, GrTLR3s, and Mx were studied in different tissues before and after virus infection by real-time quantitative RT-PCR. The transcripts of all three genes in liver were significantly increased following GCRV infection (P<0.05). The mRNA levels in liver were upregulated at 24 h post-injection for GrTLR3 and GrTLR3s, and at 12 h for Mx. The upregulated expression levels were several folds for GrTLR3s, tens of folds for GrTLR3, and hundreds of folds for Mx. By semi-quantitative RT-PCR, GrTLR3 and Mx expressed at all the developmental stages, whereas GrTLR3s could only be detected at later developmental stages. Using RNAi and transgenic techniques, GrTLR3 mediated Mx expression but GrTLR3s did not. The time-dependent upregulation of receptor and effector, and the Mx over-expression dependent on TLR3, indicated that GrTLR3 regulated Mx expression in viral infection through a configuration change in rare minnow, and its splice variant did not contribute to the process.
SubtypeArticle
KeywordGobiocypris Rarus Tlr3 Mx Transgenic Fish Rnai
Department[Su, Jianguo; Zhu, Zuoyan; Wang, Yaping; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Su, Jianguo] NW & A&F Univ, Yangling 712100, Peoples R China; [Zou, Jun] Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland
Subject AreaGenetics & Heredity ; Immunology
DOI10.1007/s00251-007-0264-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity ; Immunology
WOS SubjectGenetics & Heredity ; Immunology
WOS IDWOS:000255113300008
WOS KeywordDOUBLE-STRANDED-RNA ; MESSENGER-RNA ; ADAPTER MOLECULE ; GENE-EXPRESSION ; DANIO-RERIO ; TLR3 ; RECOGNITION ; PROTEIN ; INDUCTION ; IMMUNITY
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8170
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.NW & A&F Univ, Yangling 712100, Peoples R China
3.Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland
Recommended Citation
GB/T 7714
Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,et al. Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection[J]. IMMUNOGENETICS,2008,60(3-4):195-205.
APA Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,Zou, Jun,Hu, Wei,&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection.IMMUNOGENETICS,60(3-4),195-205.
MLA Su, Jianguo,et al."Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection".IMMUNOGENETICS 60.3-4(2008):195-205.
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