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学科主题: Toxicology
题名: Deletion of yeast CWP genes enhances cell permeability to genotoxic agents
作者: Zhang, Min1; Liang, Yuping2; Zhang, Xiaohua1; Xu, Ying1; Dai, Heping1; Xiao, Wei1, 2
通讯作者: Dai, HP, Chinese Acad Sci, Inst Hydrol, 7 Donghu Rd S, Wuhan 430072, Peoples R China
关键词: RNR3-lacZ ; yeast ; cell wall ; mannoprotein ; genotoxicity test ; sensitivity ; permeability
刊名: TOXICOLOGICAL SCIENCES
发表日期: 2008-05-01
DOI: 10.1093/toxsci/kfn034
卷: 103, 期:1, 页:68-76
收录类别: SCI
文章类型: Article
部门归属: [Zhang, Min; Zhang, Xiaohua; Xu, Ying; Dai, Heping; Xiao, Wei] Chinese Acad Sci, Inst Hydrol, Wuhan 430072, Peoples R China; [Liang, Yuping; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
摘要: We have previously reported the development of a novel genotoxic testing system based on the transcriptional response of the yeast RNR3-lacZ reporter gene to DNA damage. This system appears to be more sensitive than other similar tests in microorganisms, and is comparable with the Ames test. In an effort to further enhance detection sensitivity, we examined the effects of altering major cell wall components on cell permeability and subsequent RNR3-lacZ sensitivity to genotoxic agents. Although inactivation of single CWP genes encoding cell wall mannoproteins had little effect, the simultaneous inactivation of both CWP1 and CWP2 had profound effects on the cell wall structure and permeability. Consequently, the RNR3-lacZ detection sensitivity is markedly enhanced, especially to high molecular weight compounds such as 4-nitroquinoline-N-oxide (> sevenfold) and phleomycin (> 13-fold). In contrast, deletion of genes encoding representative membrane components or membrane transporters had minor effects on cell permeability. We conclude that the yeast cell wall mannoproteins constitute the major barrier to environmental genotoxic agents and that their removal will significantly enhance the sensitivity of RNR-lacZ as well as other yeast-based genotoxic tests.
英文摘要: We have previously reported the development of a novel genotoxic testing system based on the transcriptional response of the yeast RNR3-lacZ reporter gene to DNA damage. This system appears to be more sensitive than other similar tests in microorganisms, and is comparable with the Ames test. In an effort to further enhance detection sensitivity, we examined the effects of altering major cell wall components on cell permeability and subsequent RNR3-lacZ sensitivity to genotoxic agents. Although inactivation of single CWP genes encoding cell wall mannoproteins had little effect, the simultaneous inactivation of both CWP1 and CWP2 had profound effects on the cell wall structure and permeability. Consequently, the RNR3-lacZ detection sensitivity is markedly enhanced, especially to high molecular weight compounds such as 4-nitroquinoline-N-oxide (> sevenfold) and phleomycin (> 13-fold). In contrast, deletion of genes encoding representative membrane components or membrane transporters had minor effects on cell permeability. We conclude that the yeast cell wall mannoproteins constitute the major barrier to environmental genotoxic agents and that their removal will significantly enhance the sensitivity of RNR-lacZ as well as other yeast-based genotoxic tests.
关键词[WOS]: PLEIOTROPIC DRUG-RESISTANCE ; SACCHAROMYCES-CEREVISIAE ; MULTIDRUG-RESISTANCE ; CALCOFLUOR WHITE ; TESTING SYSTEM ; WALL POROSITY ; CONGO RED ; MEMBRANE ; MANNOPROTEINS ; SENSITIVITY
语种: 英语
WOS记录号: WOS:000254955500008
ISSN号: 1096-6080
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8160
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrol, Wuhan 430072, Peoples R China
2.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada

Recommended Citation:
Zhang, Min; Liang, Yuping; Zhang, Xiaohua; Xu, Ying; Dai, Heping; Xiao, Wei.Deletion of yeast CWP genes enhances cell permeability to genotoxic agents,TOXICOLOGICAL SCIENCES,2008,103(1):68-76
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