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学科主题: Biochemistry & Molecular Biology; Fisheries; Physiology
题名: Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps
作者: Du, X. Z.1; Zhou, L.1; Zhao, H. B.1; Wang, Y. F.1; Gui, J. F.2
通讯作者: Wang, YF, Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China
关键词: Hira gene ; cDNA cloning ; gene expression ; gynogenesis ; gibel carp
刊名: FISH PHYSIOLOGY AND BIOCHEMISTRY
发表日期: 2008-06-01
DOI: 10.1007/s10695-007-9158-1
卷: 34, 期:2, 页:175-184
收录类别: SCI
文章类型: Article
部门归属: [Du, X. Z.; Zhou, L.; Zhao, H. B.; Wang, Y. F.] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China; [Gui, J. F.] Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Fisheries ; Physiology
研究领域[WOS]: Biochemistry & Molecular Biology ; Fisheries ; Physiology
摘要: Hir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gyno-carp) and gonochoristic color crucian carp (gono-carp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.
英文摘要: Hir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gyno-carp) and gonochoristic color crucian carp (gono-carp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.
关键词[WOS]: ASSEMBLY FACTOR-I ; AURATUS-GIBELIO BLOCH ; SACCHAROMYCES-CEREVISIAE ; CARASSIUS-AURATUS ; DIGEORGE-SYNDROME ; MALE PRONUCLEUS ; DROSOPHILA-MELANOGASTER ; CANDIDATE GENE ; WD REPEAT ; PROTEINS
语种: 英语
WOS记录号: WOS:000255192800008
ISSN号: 0920-1742
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8156
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Du, X. Z.; Zhou, L.; Zhao, H. B.; Wang, Y. F.; Gui, J. F..Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps,FISH PHYSIOLOGY AND BIOCHEMISTRY,2008,34(2):175-184
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