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Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps
Du, X. Z.1; Zhou, L.1; Zhao, H. B.1; Wang, Y. F.1; Gui, J. F.2; Wang, YF, Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China
2008-06-01
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
Volume34Issue:2Pages:175-184
AbstractHir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gyno-carp) and gonochoristic color crucian carp (gono-carp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.; Hir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gyno-carp) and gonochoristic color crucian carp (gono-carp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.
SubtypeArticle
KeywordHira Gene Cdna Cloning Gene Expression Gynogenesis Gibel Carp
Department[Du, X. Z.; Zhou, L.; Zhao, H. B.; Wang, Y. F.] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China; [Gui, J. F.] Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
DOI10.1007/s10695-007-9158-1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS SubjectBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS IDWOS:000255192800008
WOS KeywordASSEMBLY FACTOR-I ; AURATUS-GIBELIO BLOCH ; SACCHAROMYCES-CEREVISIAE ; CARASSIUS-AURATUS ; DIGEORGE-SYNDROME ; MALE PRONUCLEUS ; DROSOPHILA-MELANOGASTER ; CANDIDATE GENE ; WD REPEAT ; PROTEINS
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8156
Collection期刊论文
Corresponding AuthorWang, YF, Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China
Affiliation1.Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Du, X. Z.,Zhou, L.,Zhao, H. B.,et al. Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2008,34(2):175-184.
APA Du, X. Z.,Zhou, L.,Zhao, H. B.,Wang, Y. F.,Gui, J. F.,&Wang, YF, Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.(2008).Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps.FISH PHYSIOLOGY AND BIOCHEMISTRY,34(2),175-184.
MLA Du, X. Z.,et al."Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps".FISH PHYSIOLOGY AND BIOCHEMISTRY 34.2(2008):175-184.
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