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Avian influenza virus infection induces differential expression of genes in chicken kidney
Zhang, Wanpo1; Li, Huiying2; Cheng, Guofu1; Hu, Sishun1; Li, Zili1; Bi, Dingren1; Bi, DG, Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
2008-06-01
Source PublicationRESEARCH IN VETERINARY SCIENCE
ISSN0034-5288
Volume84Issue:3Pages:374-381
AbstractThe pathogenic process of highly pathogenic avian influenza virus (HPAIV) infection is poorly understood. To explore the differential expression of kidney genes as a result of HPAIV infection, two cDNA libraries were constructed from uninfected and infected kidneys by suppression subtractive hybridization (SSH). Fifteen genes including IFN-stimulated genes (ISG12), lymphocyte antigen 6 complex locus E gene (LY6E), matrix Gla protein gene (MGP), lysozyme gene, haemopoiesis related membrane protein I gene, KIAA1259, MGC68696, G6pe-prov protein gene (G6PC), MGC4504, alcohol dehydrogenase gene (ADH), glutathione S-transferase gene (GST), sodium-dependent high-affinity dicarboxylate transporter gene (SDCT), Synaptotagmin XV (SytXV) and two novel genes were found significantly up-regulated or dramatically suppressed. Differential expression of these genes was further identified by Northern blot. Functional analysis indicated that the regulation of their expression might contribute to the pathogenic process of HPAIV infection. In contrast, the increased expression of three IFN-stimulated genes named ISG12, LY6E, and haemopoiesis related membrane protein 1 gene might reflect host defense responses. Further study showed that ISG12 protein failed to directly interact with NS1 protein of HPAIV which expressed simultaneously in the organs where HPAIV replication occurred, by use of BacterioMatch two-hybrid system. Therefore, our findings may provide new insights into understanding the molecular mechanism underlying the pathophysiological process of HPAIV infection in chicken. (c) 2007 Elsevier Ltd. All rights reserved.; The pathogenic process of highly pathogenic avian influenza virus (HPAIV) infection is poorly understood. To explore the differential expression of kidney genes as a result of HPAIV infection, two cDNA libraries were constructed from uninfected and infected kidneys by suppression subtractive hybridization (SSH). Fifteen genes including IFN-stimulated genes (ISG12), lymphocyte antigen 6 complex locus E gene (LY6E), matrix Gla protein gene (MGP), lysozyme gene, haemopoiesis related membrane protein I gene, KIAA1259, MGC68696, G6pe-prov protein gene (G6PC), MGC4504, alcohol dehydrogenase gene (ADH), glutathione S-transferase gene (GST), sodium-dependent high-affinity dicarboxylate transporter gene (SDCT), Synaptotagmin XV (SytXV) and two novel genes were found significantly up-regulated or dramatically suppressed. Differential expression of these genes was further identified by Northern blot. Functional analysis indicated that the regulation of their expression might contribute to the pathogenic process of HPAIV infection. In contrast, the increased expression of three IFN-stimulated genes named ISG12, LY6E, and haemopoiesis related membrane protein 1 gene might reflect host defense responses. Further study showed that ISG12 protein failed to directly interact with NS1 protein of HPAIV which expressed simultaneously in the organs where HPAIV replication occurred, by use of BacterioMatch two-hybrid system. Therefore, our findings may provide new insights into understanding the molecular mechanism underlying the pathophysiological process of HPAIV infection in chicken. (c) 2007 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordAvian Influenza Virus (Aiv) Chicken Suppression Subtractive Hybridization (Ssh) Gene Differential Expression
Department[Zhang, Wanpo; Cheng, Guofu; Hu, Sishun; Li, Zili; Bi, Dingren] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China; [Li, Huiying] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaVeterinary Sciences
DOI10.1016/j.rvsc.2007.05.015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaVeterinary Sciences
WOS SubjectVeterinary Sciences
WOS IDWOS:000255832900010
WOS KeywordSUPPRESSION SUBTRACTIVE HYBRIDIZATION ; A VIRUSES ; PROTEIN ; IDENTIFICATION ; H5N1 ; ISG12 ; MICE ; GLUTATHIONE ; PROFILE ; CLONING
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8132
Collection期刊论文
Corresponding AuthorBi, DG, Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
Affiliation1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Wanpo,Li, Huiying,Cheng, Guofu,et al. Avian influenza virus infection induces differential expression of genes in chicken kidney[J]. RESEARCH IN VETERINARY SCIENCE,2008,84(3):374-381.
APA Zhang, Wanpo.,Li, Huiying.,Cheng, Guofu.,Hu, Sishun.,Li, Zili.,...&Bi, DG, Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China.(2008).Avian influenza virus infection induces differential expression of genes in chicken kidney.RESEARCH IN VETERINARY SCIENCE,84(3),374-381.
MLA Zhang, Wanpo,et al."Avian influenza virus infection induces differential expression of genes in chicken kidney".RESEARCH IN VETERINARY SCIENCE 84.3(2008):374-381.
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