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Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China
Zhao, Zhe; Ke, Fei; Li, Zhengqiu; Gui, Jianfang; Zhang, Qiya; Zhang, Q, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China
2008-06-01
Source PublicationARCHIVES OF VIROLOGY
ISSN0304-8608
Volume153Issue:6Pages:1143-1148
AbstractA birnavirus strain, Paralichthys olivaceus birnavirus (POBV), was isolated and characterized from cultured flounder in China, and its complete genomic sequence was subsequently determined. The virus could induce cytopathic effects (CPE) in four of seven fish cell lines and was resistant to chloroform, 5-iodo-2'-deoxyuridine, acid and alkaline pH, and heat treatment. Purified virus particles had a typical icosahedral shape, with a diameter of approximately 55-60 nm. The genomic segments A and B of POBV were 3,091 and 2,780 bp in length and shared many of the features of the members of the family Birnaviridae. Segment A contained two partially overlapping ORFs encoding a polyprotein, pVP2-VP4-VP3, and a nonstructural protein, VP5, while segment B had only one ORF encoding for the VP1, a viral RNA-dependent RNA polymerase (RdRp). This is the first report about a birnavirus strain from a new non-salmonid host in China and its complete genome sequence.; A birnavirus strain, Paralichthys olivaceus birnavirus (POBV), was isolated and characterized from cultured flounder in China, and its complete genomic sequence was subsequently determined. The virus could induce cytopathic effects (CPE) in four of seven fish cell lines and was resistant to chloroform, 5-iodo-2'-deoxyuridine, acid and alkaline pH, and heat treatment. Purified virus particles had a typical icosahedral shape, with a diameter of approximately 55-60 nm. The genomic segments A and B of POBV were 3,091 and 2,780 bp in length and shared many of the features of the members of the family Birnaviridae. Segment A contained two partially overlapping ORFs encoding a polyprotein, pVP2-VP4-VP3, and a nonstructural protein, VP5, while segment B had only one ORF encoding for the VP1, a viral RNA-dependent RNA polymerase (RdRp). This is the first report about a birnavirus strain from a new non-salmonid host in China and its complete genome sequence.
SubtypeArticle
KeywordPancreatic Necrosis Virus Cell-line Molecular Characterization Cleavage Sites Disease Virus Vp4 Protease Virulence Fish Rhabdovirus Expression
Department[Zhao, Zhe; Ke, Fei; Li, Zhengqiu; Gui, Jianfang; Zhang, Qiya] Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China
Subject AreaVirology
DOI10.1007/s00705-008-0075-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000256089000016
WOS KeywordPANCREATIC NECROSIS VIRUS ; CELL-LINE ; MOLECULAR CHARACTERIZATION ; CLEAVAGE SITES ; DISEASE VIRUS ; VP4 PROTEASE ; VIRULENCE ; FISH ; RHABDOVIRUS ; EXPRESSION
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8128
Collection期刊论文
Corresponding AuthorZhang, Q, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China
AffiliationGrad Univ, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Zhe,Ke, Fei,Li, Zhengqiu,et al. Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China[J]. ARCHIVES OF VIROLOGY,2008,153(6):1143-1148.
APA Zhao, Zhe,Ke, Fei,Li, Zhengqiu,Gui, Jianfang,Zhang, Qiya,&Zhang, Q, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China.(2008).Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China.ARCHIVES OF VIROLOGY,153(6),1143-1148.
MLA Zhao, Zhe,et al."Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China".ARCHIVES OF VIROLOGY 153.6(2008):1143-1148.
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