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Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique
Pei, De-Sheng; Sun, Yong-Hua; Long, Yong; Zhu, Zuo-Yan; Sun, YH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
2008-06-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume35Issue:2Pages:139-143
AbstractExternal guide sequence (EGS) technique, a branch of ribozyme strategy, can be enticed to cleave the target mRNA by forming a tRNA-like structure. In the present study, no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. Transient expression of pH1-m3/4 ntl-EGS or pH1-3/4 ntl-EGS produced the full no tail phenotype at long-pec stage in proportion as 24 or 35%, respectively. As is expected that the full-length ntl mRNA of embryos at 50% epiboly stage decreased relative to control when injected the embryos with 3/4 EGS or m3/4 EGS RNA. Interestingly, ntl RNA transcripts, including the cleaved by EGS and the untouched, increased. Taken together, these results indicate that EGS strategy can work in zebrafish in vivo and becomes a potential tool for degradation of targeted mRNAs.; External guide sequence (EGS) technique, a branch of ribozyme strategy, can be enticed to cleave the target mRNA by forming a tRNA-like structure. In the present study, no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. Transient expression of pH1-m3/4 ntl-EGS or pH1-3/4 ntl-EGS produced the full no tail phenotype at long-pec stage in proportion as 24 or 35%, respectively. As is expected that the full-length ntl mRNA of embryos at 50% epiboly stage decreased relative to control when injected the embryos with 3/4 EGS or m3/4 EGS RNA. Interestingly, ntl RNA transcripts, including the cleaved by EGS and the untouched, increased. Taken together, these results indicate that EGS strategy can work in zebrafish in vivo and becomes a potential tool for degradation of targeted mRNAs.
SubtypeArticle
KeywordExternal Guide Sequence No Tail Rnase p Zebrafish
Department[Pei, De-Sheng; Sun, Yong-Hua; Long, Yong; Zhu, Zuo-Yan] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology
DOI10.1007/s11033-007-9063-9
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000256082300009
WOS KeywordRNASE-P ; RIBONUCLEASE P ; MODEL ; CLEAVAGE ; SIRNAS ; ASTHMA ; CELLS
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8114
Collection期刊论文
Corresponding AuthorSun, YH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Pei, De-Sheng,Sun, Yong-Hua,Long, Yong,et al. Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique[J]. MOLECULAR BIOLOGY REPORTS,2008,35(2):139-143.
APA Pei, De-Sheng,Sun, Yong-Hua,Long, Yong,Zhu, Zuo-Yan,&Sun, YH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China.(2008).Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique.MOLECULAR BIOLOGY REPORTS,35(2),139-143.
MLA Pei, De-Sheng,et al."Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique".MOLECULAR BIOLOGY REPORTS 35.2(2008):139-143.
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