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Microcystin-induced variations in transcription of GSTs in an omnivorous freshwater fish, goldfish
Li, Guangyu; Xie, Ping; Fu, Juan; Hao, Le; Xiong, Qian; Li, Huiying; Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
2008-06-02
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume88Issue:1Pages:75-80
AbstractThe glutathione S-transferases are important enzymes in the microcystin-induced detoxication processes. In this experiment, we cloned the full-length cDNA of alpha, pi and theta-class-like glutathione S-transferase genes from goldfish (Carassius auratus Q. Their derived amino acid sequences were clustered with other vertebrate alpha, pi and theta-class GSTs in a phylogenetic tree and the goldfish GST sequences have the highest similarity with those from common carp and zebrafish. Goldfish were i.p. injected with microcystins extract at two doses (50 and 200 mu g kg(-1) BW MC-LReq) and the relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GST alpha was suppressed in both liver and intestine, but induced in the kidney. Decreased transcription of GST theta was detected in liver, kidney and intestine in the low-dose group. The transcription of GST pi was suppressed in liver and intestine post-injection in both dose groups. These results suggested that the transcription of GST isoforms varied in different ways within an organ and among organs of goldfish exposed to MCs. (C) 2008 Elsevier B.V. All rights reserved.; The glutathione S-transferases are important enzymes in the microcystin-induced detoxication processes. In this experiment, we cloned the full-length cDNA of alpha, pi and theta-class-like glutathione S-transferase genes from goldfish (Carassius auratus Q. Their derived amino acid sequences were clustered with other vertebrate alpha, pi and theta-class GSTs in a phylogenetic tree and the goldfish GST sequences have the highest similarity with those from common carp and zebrafish. Goldfish were i.p. injected with microcystins extract at two doses (50 and 200 mu g kg(-1) BW MC-LReq) and the relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GST alpha was suppressed in both liver and intestine, but induced in the kidney. Decreased transcription of GST theta was detected in liver, kidney and intestine in the low-dose group. The transcription of GST pi was suppressed in liver and intestine post-injection in both dose groups. These results suggested that the transcription of GST isoforms varied in different ways within an organ and among organs of goldfish exposed to MCs. (C) 2008 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicrocystin Glutathione S-transferases Real-time Pcr Goldfish Carassius Auratus L.
Department[Li, Guangyu; Xie, Ping; Fu, Juan; Hao, Le; Xiong, Qian; Li, Huiying] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Subject AreaMarine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2008.03.007
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000257005700010
WOS KeywordPHYTOPLANKTIVOROUS SILVER CARP ; S-TRANSFERASE GENES ; DANIO-RERIO ; RAINBOW-TROUT ; INTRAPERITONEAL INJECTION ; GLUTATHIONE TRANSFERASES ; ULTRASTRUCTURAL-CHANGES ; EXTRACTED MICROCYSTINS ; CARASSIUS-AURATUS ; CYPRINUS-CARPIO
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8100
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Guangyu,Xie, Ping,Fu, Juan,et al. Microcystin-induced variations in transcription of GSTs in an omnivorous freshwater fish, goldfish[J]. AQUATIC TOXICOLOGY,2008,88(1):75-80.
APA Li, Guangyu.,Xie, Ping.,Fu, Juan.,Hao, Le.,Xiong, Qian.,...&Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.(2008).Microcystin-induced variations in transcription of GSTs in an omnivorous freshwater fish, goldfish.AQUATIC TOXICOLOGY,88(1),75-80.
MLA Li, Guangyu,et al."Microcystin-induced variations in transcription of GSTs in an omnivorous freshwater fish, goldfish".AQUATIC TOXICOLOGY 88.1(2008):75-80.
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