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Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors
Yan Qingyun1,2; Yu Yuhe1; Feng Weisong1; Yu Zhigang3; Chen Hongtao3; Yu, YH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008
Source PublicationJOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
ISSN1001-0742
Volume20Issue:6Pages:732-738
AbstractTo explore the relationships between community composition and the environment in a reservoir ecosystem, plankton communities from the Three Gorges Reservoir Region were studied by PCR-denaturing gradient gel electrophoresis fingerprinting. Bacterial and eukaryotic operational taxonomic units (OTUs), generated by DGGE analysis of the PCR-amplified 16S and 18S rRNA genes, were used as surrogates for the dominant "biodiversity units". OTU composition among the sites was heterogeneous; 46.7% of the total bacteria] OTUs (45) and 64.1% of the eukaryotic OTUs (39) were identified in less than half of the sampling sites. Unweighted pair group method with arithmetic averages (UPGMA) clustering of the OTUs suggested that the plankton communities in the Xiangxi Rive sites were not always significantly different from those from the Yangtze River sites, despite clear differences in their environmental characterizations. Canonical correspondence analysis (CCA) was applied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes suggested that the bacterial community composition was primarily correlated with the variables of NO3--N, dissolved oxygen (DO), and SiO32--Si, whereas, the eukaryotic community was mainly correlated with the concentrations of DO, PO43--P, and SiO32--Si.; To explore the relationships between community composition and the environment in a reservoir ecosystem, plankton communities from the Three Gorges Reservoir Region were studied by PCR-denaturing gradient gel electrophoresis fingerprinting. Bacterial and eukaryotic operational taxonomic units (OTUs), generated by DGGE analysis of the PCR-amplified 16S and 18S rRNA genes, were used as surrogates for the dominant "biodiversity units". OTU composition among the sites was heterogeneous; 46.7% of the total bacteria] OTUs (45) and 64.1% of the eukaryotic OTUs (39) were identified in less than half of the sampling sites. Unweighted pair group method with arithmetic averages (UPGMA) clustering of the OTUs suggested that the plankton communities in the Xiangxi Rive sites were not always significantly different from those from the Yangtze River sites, despite clear differences in their environmental characterizations. Canonical correspondence analysis (CCA) was applied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes suggested that the bacterial community composition was primarily correlated with the variables of NO3--N, dissolved oxygen (DO), and SiO32--Si, whereas, the eukaryotic community was mainly correlated with the concentrations of DO, PO43--P, and SiO32--Si.
SubtypeArticle
KeywordPlankton Community Environmental Factors Denaturing Gradient Gel Electrophoresis (Dgge) Spatial Pattern Three Gorges Reservoir Region
Department[Yan Qingyun; Yu Yuhe; Feng Weisong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Yan Qingyun] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Yu Zhigang; Chen Hongtao] Ocean Univ Qingdao, Qingdao 266003, Peoples R China
Subject AreaEnvironmental Sciences
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000256858400015
WOS KeywordGRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; GENETIC DIVERSITY ; LAKES ; PHYTOPLANKTON ; ECOLOGY ; MICROORGANISMS ; ASSEMBLAGES ; POPULATIONS ; INDICATORS
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8096
Collection期刊论文
Corresponding AuthorYu, YH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Ocean Univ China, Qingdao 266003, Peoples R China
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GB/T 7714
Yan Qingyun,Yu Yuhe,Feng Weisong,et al. Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(6):732-738.
APA Yan Qingyun,Yu Yuhe,Feng Weisong,Yu Zhigang,Chen Hongtao,&Yu, YH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,20(6),732-738.
MLA Yan Qingyun,et al."Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 20.6(2008):732-738.
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