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Detection of the hepatotoxic microcystins in 36 kinds of cyanobacteria Spirulina food products in China
Jiang, Y.; Xie, P.; Chen, J.; Liang, G.; Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan, Peoples R China
2008
Source PublicationFOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
ISSN1944-0049
Volume25Issue:7Pages:885-894
AbstractGel filtration chromatography, ultra-filtration, and solid-phase extraction silica gel clean-up were evaluated for their ability to remove microcystins selectively from extracts of cyanobacteria Spirulina samples after using the reversed-phase octadecylsilyl ODS cartridge for subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The reversed-phase ODS cartridge/silica gel combination were effective and the optimal wash and elution conditions were: H2O (wash), 20% methanol in water (wash), and 90% methanol in water (elution) for the reversed-phase ODS cartridge, followed by 80% methanol in water elution in the silica gel cartridge. The presence of microcystins in 36 kinds of cyanobacteria Spirulina health food samples obtained from various retail outlets in China were detected by LC-MS/MS, and 34 samples (94%) contained microcystins ranging from 2 to 163 ng g(-1) (mean=1427 ng g(-1)), which were significantly lower than microcystins present in blue green alga products previously reported. MC-RR-which contains two molecules of arginine (R)-(in 94.4% samples) was the predominant microcystin, followed by MC-LR-where L is leucine-(30.6%) and MC-YR-where Y is tyrose-(27.8%). The possible potential health risks from chronic exposure to microcystins from contaminated cyanobacteria Spirulina health food should not be ignored, even if the toxin concentrations were low. The method presented herein is proposed to detect microcystins present in commercial cyanobacteria Spirulina samples.; Gel filtration chromatography, ultra-filtration, and solid-phase extraction silica gel clean-up were evaluated for their ability to remove microcystins selectively from extracts of cyanobacteria Spirulina samples after using the reversed-phase octadecylsilyl ODS cartridge for subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The reversed-phase ODS cartridge/silica gel combination were effective and the optimal wash and elution conditions were: H(2)O (wash), 20% methanol in water (wash), and 90% methanol in water (elution) for the reversed-phase ODS cartridge, followed by 80% methanol in water elution in the silica gel cartridge. The presence of microcystins in 36 kinds of cyanobacteria Spirulina health food samples obtained from various retail outlets in China were detected by LC-MS/MS, and 34 samples (94%) contained microcystins ranging from 2 to 163 ng g(-1) (mean=1427 ng g(-1)), which were significantly lower than microcystins present in blue green alga products previously reported. MC-RR-which contains two molecules of arginine (R)-(in 94.4% samples) was the predominant microcystin, followed by MC-LR-where L is leucine-(30.6%) and MC-YR-where Y is tyrose-(27.8%). The possible potential health risks from chronic exposure to microcystins from contaminated cyanobacteria Spirulina health food should not be ignored, even if the toxin concentrations were low. The method presented herein is proposed to detect microcystins present in commercial cyanobacteria Spirulina samples.
SubtypeArticle
KeywordCyanobacteria Spirulina Health Food Microcystins Liquid ChromaTography Coupled To Tandem Mass Spectrometry (Lc- Ms/ms) Clean-up
Department[Jiang, Y.; Xie, P.; Chen, J.; Liang, G.] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan, Peoples R China
Subject AreaChemistry ; Applied ; Food Science & Technology ; Toxicology
DOI10.1080/02652030701822045
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Food Science & Technology ; Toxicology
WOS SubjectChemistry, Applied ; Food Science & Technology ; Toxicology
WOS IDWOS:000257087700008
WOS KeywordBLUE-GREEN-ALGAE ; LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; MOUSE-LIVER ; FLOS-AQUAE ; LR ; IONIZATION ; BIOSYNTHESIS ; PURIFICATION ; SUPPLEMENTS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8086
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan, Peoples R China
AffiliationChinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Y.,Xie, P.,Chen, J.,et al. Detection of the hepatotoxic microcystins in 36 kinds of cyanobacteria Spirulina food products in China[J]. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT,2008,25(7):885-894.
APA Jiang, Y.,Xie, P.,Chen, J.,Liang, G.,&Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan, Peoples R China.(2008).Detection of the hepatotoxic microcystins in 36 kinds of cyanobacteria Spirulina food products in China.FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT,25(7),885-894.
MLA Jiang, Y.,et al."Detection of the hepatotoxic microcystins in 36 kinds of cyanobacteria Spirulina food products in China".FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT 25.7(2008):885-894.
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