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Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp
Wang, Yuzhen1,2; Zhang, Xiaohua1; Yuan, Li1; Xu, Tao1,2; Rao, Yu1,2; Li, Jia1,2; Dai, Heping1; Dai, HP, Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, 7 So E Lake Rd, Wuhan 430072, Peoples R China
2008-08-08
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume372Issue:4Pages:902-906
AbstractWhite spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV. In this study, a recombinant antibody library, as single-chain fragment variable (scFv) format, displayed on phage was constructed using mRNA from spleen cells of mice immunized with-full-length VP28 expressed in Escherichia coli. After several rounds of panning, six scFv antibodies specifically binding to the epitopes in the N-terminal, middle, and C-terminal regions of VP28, respectively, were isolated from the library. Using these scFv antibodies as tools, the epitopes in VP28 were located on the envelope of the virion by immuno-electron Microscopy, Neutralization assay with these antibodies in vitro suggested that these epitopes may not be the attachment site of WSSV to host cell receptor. This study provides a new way to investigate the structure and function of the envelope proteins of WSSV. (c) 2008 Published by Elsevier Inc.; White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV. In this study, a recombinant antibody library, as single-chain fragment variable (scFv) format, displayed on phage was constructed using mRNA from spleen cells of mice immunized with-full-length VP28 expressed in Escherichia coli. After several rounds of panning, six scFv antibodies specifically binding to the epitopes in the N-terminal, middle, and C-terminal regions of VP28, respectively, were isolated from the library. Using these scFv antibodies as tools, the epitopes in VP28 were located on the envelope of the virion by immuno-electron Microscopy, Neutralization assay with these antibodies in vitro suggested that these epitopes may not be the attachment site of WSSV to host cell receptor. This study provides a new way to investigate the structure and function of the envelope proteins of WSSV. (c) 2008 Published by Elsevier Inc.
SubtypeArticle
KeywordWssv Vp28 Phage Display Scfv Antibody Shrimp
Department[Wang, Yuzhen; Zhang, Xiaohua; Yuan, Li; Xu, Tao; Rao, Yu; Li, Jia; Dai, Heping] Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China; [Wang, Yuzhen; Xu, Tao; Rao, Yu; Li, Jia] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Biophysics
DOI10.1016/j.bbrc.2008.05.152
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000257419500072
WOS KeywordPENAEUS-MONODON ; GENOME SEQUENCE ; WSSV ; IDENTIFICATION ; NEUTRALIZATION ; MORPHOGENESIS ; PURIFICATION ; INFECTION ; GENES ; CELLS
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8082
Collection期刊论文
Corresponding AuthorDai, HP, Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, 7 So E Lake Rd, Wuhan 430072, Peoples R China
Affiliation1.Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yuzhen,Zhang, Xiaohua,Yuan, Li,et al. Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,372(4):902-906.
APA Wang, Yuzhen.,Zhang, Xiaohua.,Yuan, Li.,Xu, Tao.,Rao, Yu.,...&Dai, HP, Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, 7 So E Lake Rd, Wuhan 430072, Peoples R China.(2008).Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,372(4),902-906.
MLA Wang, Yuzhen,et al."Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 372.4(2008):902-906.
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