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学科主题: Biochemistry & Molecular Biology; Biophysics
题名: Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp
作者: Wang, Yuzhen1, 2; Zhang, Xiaohua1; Yuan, Li1; Xu, Tao1, 2; Rao, Yu1, 2; Li, Jia1, 2; Dai, Heping1
通讯作者: Dai, HP, Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, 7 So E Lake Rd, Wuhan 430072, Peoples R China
关键词: WSSV ; VP28 ; phage display ; ScFv antibody ; shrimp
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2008-08-08
DOI: 10.1016/j.bbrc.2008.05.152
卷: 372, 期:4, 页:902-906
收录类别: SCI
文章类型: Article
部门归属: [Wang, Yuzhen; Zhang, Xiaohua; Yuan, Li; Xu, Tao; Rao, Yu; Li, Jia; Dai, Heping] Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China; [Wang, Yuzhen; Xu, Tao; Rao, Yu; Li, Jia] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
摘要: White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV. In this study, a recombinant antibody library, as single-chain fragment variable (scFv) format, displayed on phage was constructed using mRNA from spleen cells of mice immunized with-full-length VP28 expressed in Escherichia coli. After several rounds of panning, six scFv antibodies specifically binding to the epitopes in the N-terminal, middle, and C-terminal regions of VP28, respectively, were isolated from the library. Using these scFv antibodies as tools, the epitopes in VP28 were located on the envelope of the virion by immuno-electron Microscopy, Neutralization assay with these antibodies in vitro suggested that these epitopes may not be the attachment site of WSSV to host cell receptor. This study provides a new way to investigate the structure and function of the envelope proteins of WSSV. (c) 2008 Published by Elsevier Inc.
英文摘要: White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV. In this study, a recombinant antibody library, as single-chain fragment variable (scFv) format, displayed on phage was constructed using mRNA from spleen cells of mice immunized with-full-length VP28 expressed in Escherichia coli. After several rounds of panning, six scFv antibodies specifically binding to the epitopes in the N-terminal, middle, and C-terminal regions of VP28, respectively, were isolated from the library. Using these scFv antibodies as tools, the epitopes in VP28 were located on the envelope of the virion by immuno-electron Microscopy, Neutralization assay with these antibodies in vitro suggested that these epitopes may not be the attachment site of WSSV to host cell receptor. This study provides a new way to investigate the structure and function of the envelope proteins of WSSV. (c) 2008 Published by Elsevier Inc.
关键词[WOS]: PENAEUS-MONODON ; GENOME SEQUENCE ; WSSV ; IDENTIFICATION ; NEUTRALIZATION ; MORPHOGENESIS ; PURIFICATION ; INFECTION ; GENES ; CELLS
语种: 英语
WOS记录号: WOS:000257419500072
ISSN号: 0006-291X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8082
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Wang, Yuzhen; Zhang, Xiaohua; Yuan, Li; Xu, Tao; Rao, Yu; Li, Jia; Dai, Heping.Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp,BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,372(4):902-906
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