Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis) | |
Zheng, J. S.1; Liao, X. L.1,2; Tong, J. G.1,2; Du, H. J.1; Milinkovitch, M. C.3; Wang, D.1; Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China | |
2008 | |
Source Publication | CONSERVATION GENETICS
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ISSN | 1566-0621 |
Volume | 9Issue:4Pages:1007-1009 |
Abstract | The finless porpoise (Neophocaena phocaenoides) is one of the smallest cetacean species widely distributed in the shallow coastal waters of the Indo-Pacific Oceans. The population size of the Yangtze subspecies (N. p. asiaeorientalis) has sharply decreased in the last two decades and access to objective data on its population structure and genetic diversity would be of great assistance for their proper management. Here we report on the isolation of nine polymorphic microsatellite using the "Fast Isolation by AFLP of Sequences Containing repeats" (FIASCO) protocol. Polymorphism was assessed using 30 porpoise individuals randomly sampled in the Yangtze River. The number of alleles per locus varies from 2 to 9, with an average value of 5.56, whereas the ranges of observed and expected heterozygosi ties were 0.300-0.633 (mean 0.496) and 0.473-0.804 (mean 0.659), respectively.; The finless porpoise (Neophocaena phocaenoides) is one of the smallest cetacean species widely distributed in the shallow coastal waters of the Indo-Pacific Oceans. The population size of the Yangtze subspecies (N. p. asiaeorientalis) has sharply decreased in the last two decades and access to objective data on its population structure and genetic diversity would be of great assistance for their proper management. Here we report on the isolation of nine polymorphic microsatellite using the "Fast Isolation by AFLP of Sequences Containing repeats" (FIASCO) protocol. Polymorphism was assessed using 30 porpoise individuals randomly sampled in the Yangtze River. The number of alleles per locus varies from 2 to 9, with an average value of 5.56, whereas the ranges of observed and expected heterozygosi ties were 0.300-0.633 (mean 0.496) and 0.473-0.804 (mean 0.659), respectively. |
Subtype | Article |
Keyword | Yangtze Finless Porpoise Neophocoena Phocaenoides Asiaeorientalis Fiasco Microsatellite Conservation Genetics |
Department | [Zheng, J. S.; Liao, X. L.; Tong, J. G.; Du, H. J.; Wang, D.] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liao, X. L.; Tong, J. G.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Milinkovitch, M. C.] Free Univ Brussels, Inst Mol Biol & Med, Lab Evolutionary Genet, B-6041 Gosselies, Belgium |
Subject Area | Biodiversity Conservation ; Genetics & Heredity |
DOI | 10.1007/s10592-007-9435-7 |
WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
Indexed By | SCI |
Language | 英语 |
WOS Research Area | Biodiversity & Conservation ; Genetics & Heredity |
WOS Subject | Biodiversity Conservation ; Genetics & Heredity |
WOS ID | WOS:000257420700030 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/152342/8078 |
Collection | 期刊论文 |
Corresponding Author | Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Free Univ Brussels, Inst Mol Biol & Med, Lab Evolutionary Genet, B-6041 Gosselies, Belgium |
Recommended Citation GB/T 7714 | Zheng, J. S.,Liao, X. L.,Tong, J. G.,et al. Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)[J]. CONSERVATION GENETICS,2008,9(4):1007-1009. |
APA | Zheng, J. S..,Liao, X. L..,Tong, J. G..,Du, H. J..,Milinkovitch, M. C..,...&Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis).CONSERVATION GENETICS,9(4),1007-1009. |
MLA | Zheng, J. S.,et al."Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)".CONSERVATION GENETICS 9.4(2008):1007-1009. |
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