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Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)
Zheng, J. S.1; Liao, X. L.1,2; Tong, J. G.1,2; Du, H. J.1; Milinkovitch, M. C.3; Wang, D.1; Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008
Source PublicationCONSERVATION GENETICS
ISSN1566-0621
Volume9Issue:4Pages:1007-1009
AbstractThe finless porpoise (Neophocaena phocaenoides) is one of the smallest cetacean species widely distributed in the shallow coastal waters of the Indo-Pacific Oceans. The population size of the Yangtze subspecies (N. p. asiaeorientalis) has sharply decreased in the last two decades and access to objective data on its population structure and genetic diversity would be of great assistance for their proper management. Here we report on the isolation of nine polymorphic microsatellite using the "Fast Isolation by AFLP of Sequences Containing repeats" (FIASCO) protocol. Polymorphism was assessed using 30 porpoise individuals randomly sampled in the Yangtze River. The number of alleles per locus varies from 2 to 9, with an average value of 5.56, whereas the ranges of observed and expected heterozygosi ties were 0.300-0.633 (mean 0.496) and 0.473-0.804 (mean 0.659), respectively.; The finless porpoise (Neophocaena phocaenoides) is one of the smallest cetacean species widely distributed in the shallow coastal waters of the Indo-Pacific Oceans. The population size of the Yangtze subspecies (N. p. asiaeorientalis) has sharply decreased in the last two decades and access to objective data on its population structure and genetic diversity would be of great assistance for their proper management. Here we report on the isolation of nine polymorphic microsatellite using the "Fast Isolation by AFLP of Sequences Containing repeats" (FIASCO) protocol. Polymorphism was assessed using 30 porpoise individuals randomly sampled in the Yangtze River. The number of alleles per locus varies from 2 to 9, with an average value of 5.56, whereas the ranges of observed and expected heterozygosi ties were 0.300-0.633 (mean 0.496) and 0.473-0.804 (mean 0.659), respectively.
SubtypeArticle
KeywordYangtze Finless Porpoise Neophocoena Phocaenoides Asiaeorientalis Fiasco Microsatellite Conservation Genetics
Department[Zheng, J. S.; Liao, X. L.; Tong, J. G.; Du, H. J.; Wang, D.] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liao, X. L.; Tong, J. G.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Milinkovitch, M. C.] Free Univ Brussels, Inst Mol Biol & Med, Lab Evolutionary Genet, B-6041 Gosselies, Belgium
Subject AreaBiodiversity Conservation ; Genetics & Heredity
DOI10.1007/s10592-007-9435-7
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000257420700030
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8078
Collection期刊论文
Corresponding AuthorWang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Free Univ Brussels, Inst Mol Biol & Med, Lab Evolutionary Genet, B-6041 Gosselies, Belgium
Recommended Citation
GB/T 7714
Zheng, J. S.,Liao, X. L.,Tong, J. G.,et al. Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)[J]. CONSERVATION GENETICS,2008,9(4):1007-1009.
APA Zheng, J. S..,Liao, X. L..,Tong, J. G..,Du, H. J..,Milinkovitch, M. C..,...&Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis).CONSERVATION GENETICS,9(4),1007-1009.
MLA Zheng, J. S.,et al."Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)".CONSERVATION GENETICS 9.4(2008):1007-1009.
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