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学科主题: Fisheries; Marine & Freshwater Biology; Veterinary Sciences
题名: Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus
作者: Lue, A-J1, 2; Li, Z-Q1; Zhang, Q-Y1
通讯作者: Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Ctenopharyngodon idellus ; cutaneous antibodies ; detection ; grass carp ; Scophthalmus maximus rhabdovirus ; skin culture
刊名: JOURNAL OF FISH DISEASES
发表日期: 2008-08-01
DOI: 10.1111/j.1365-2761.2008.00919.x
卷: 31, 期:8, 页:559-565
收录类别: SCI
文章类型: Article
部门归属: [Lue, A-J; Li, Z-Q; Zhang, Q-Y] Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Lue, A-J] Xuzhou Normal Univ, Sch Life Sci, Xuzhou, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: This study determined whether cutaneous antibodies were present in excised skin explants of grass carp, Ctenopharyngodon idella, immune to Scophthalmus maximus rhabdovirus (SMRV). Culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (iELISA), Western blot, indirect immunofluorescent assay (IFA) and flow cytometry (FCM). iELISA showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized grass carp. The phosphoprotein and matrix protein antigens of purified SMRV proteins were recognized by cutaneous antibodies from skin culture fluid using Western blot. The skin culture fluid produced staining signals in viral assembly sites and cytoplasm of SMRV-infected epithelioma papulosum cyprini (EPC) cells by IFA. FCM showed that 4.39% SMRV-infected EPC cells were detected, while non-specific reaction was seen in 2% of control cells. This is the first description of cutaneous antibodies against SMRV in grass carp.
英文摘要: This study determined whether cutaneous antibodies were present in excised skin explants of grass carp, Ctenopharyngodon idella, immune to Scophthalmus maximus rhabdovirus (SMRV). Culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (iELISA), Western blot, indirect immunofluorescent assay (IFA) and flow cytometry (FCM). iELISA showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized grass carp. The phosphoprotein and matrix protein antigens of purified SMRV proteins were recognized by cutaneous antibodies from skin culture fluid using Western blot. The skin culture fluid produced staining signals in viral assembly sites and cytoplasm of SMRV-infected epithelioma papulosum cyprini (EPC) cells by IFA. FCM showed that 4.39% SMRV-infected EPC cells were detected, while non-specific reaction was seen in 2% of control cells. This is the first description of cutaneous antibodies against SMRV in grass carp.
关键词[WOS]: ICTALURUS-PUNCTATUS RAFINESQUE ; CHANNEL CATFISH ; ICHTHYOPHTHIRIUS-MULTIFILIIS ; FLAVOBACTERIUM-COLUMNARE ; MARINE FISH ; L. ; MUCOSAL ; IMMUNIZATION ; APOPTOSIS ; RESPONSES
语种: 英语
WOS记录号: WOS:000257493600001
ISSN号: 0140-7775
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8074
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou, Peoples R China

Recommended Citation:
Lue, A-J; Li, Z-Q; Zhang, Q-Y.Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus,JOURNAL OF FISH DISEASES,2008,31(8):559-565
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