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Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus
Lue, A-J1,2; Li, Z-Q1; Zhang, Q-Y1; Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-08-01
Source PublicationJOURNAL OF FISH DISEASES
ISSN0140-7775
Volume31Issue:8Pages:559-565
AbstractThis study determined whether cutaneous antibodies were present in excised skin explants of grass carp, Ctenopharyngodon idella, immune to Scophthalmus maximus rhabdovirus (SMRV). Culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (iELISA), Western blot, indirect immunofluorescent assay (IFA) and flow cytometry (FCM). iELISA showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized grass carp. The phosphoprotein and matrix protein antigens of purified SMRV proteins were recognized by cutaneous antibodies from skin culture fluid using Western blot. The skin culture fluid produced staining signals in viral assembly sites and cytoplasm of SMRV-infected epithelioma papulosum cyprini (EPC) cells by IFA. FCM showed that 4.39% SMRV-infected EPC cells were detected, while non-specific reaction was seen in 2% of control cells. This is the first description of cutaneous antibodies against SMRV in grass carp.; This study determined whether cutaneous antibodies were present in excised skin explants of grass carp, Ctenopharyngodon idella, immune to Scophthalmus maximus rhabdovirus (SMRV). Culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (iELISA), Western blot, indirect immunofluorescent assay (IFA) and flow cytometry (FCM). iELISA showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized grass carp. The phosphoprotein and matrix protein antigens of purified SMRV proteins were recognized by cutaneous antibodies from skin culture fluid using Western blot. The skin culture fluid produced staining signals in viral assembly sites and cytoplasm of SMRV-infected epithelioma papulosum cyprini (EPC) cells by IFA. FCM showed that 4.39% SMRV-infected EPC cells were detected, while non-specific reaction was seen in 2% of control cells. This is the first description of cutaneous antibodies against SMRV in grass carp.
SubtypeArticle
KeywordCtenopharyngodon Idellus Cutaneous Antibodies Detection Grass Carp Scophthalmus Maximus Rhabdovirus Skin Culture
Department[Lue, A-J; Li, Z-Q; Zhang, Q-Y] Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Lue, A-J] Xuzhou Normal Univ, Sch Life Sci, Xuzhou, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1111/j.1365-2761.2008.00919.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000257493600001
WOS KeywordICTALURUS-PUNCTATUS RAFINESQUE ; CHANNEL CATFISH ; ICHTHYOPHTHIRIUS-MULTIFILIIS ; FLAVOBACTERIUM-COLUMNARE ; MARINE FISH ; L. ; MUCOSAL ; IMMUNIZATION ; APOPTOSIS ; RESPONSES
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8074
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou, Peoples R China
Recommended Citation
GB/T 7714
Lue, A-J,Li, Z-Q,Zhang, Q-Y,et al. Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus[J]. JOURNAL OF FISH DISEASES,2008,31(8):559-565.
APA Lue, A-J,Li, Z-Q,Zhang, Q-Y,&Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus.JOURNAL OF FISH DISEASES,31(8),559-565.
MLA Lue, A-J,et al."Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus".JOURNAL OF FISH DISEASES 31.8(2008):559-565.
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