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Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus
Su, Jianguo1,2; Zhu, Zuoyan1; Wang, Yaping1; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-07-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume25Issue:1-2Pages:106-113
AbstractDouble-stranded RNA-activated protein kinase (PKR) plays an important rote in interferon-induced antiviral responses, and is also involved in intracellular signaling pathways, including the apoptosis, proliferation, and transcription pathways. In the present study, a PKR-like gene was cloned and characterized from rare minnow Gobiocypris rarus. The full length of the rare minnow PKR-like (GrPKZ) cDNA is 1946 bp in Length and encodes a polypeptide of 503 amino acids with an estimated molecular mass of 57,355 Da and a predicted isoelectric point of 5.83. Analysis of the deduced amino acid sequence indicated that the mature peptide contains two Zalpha domains and one S_TKc domain, and is most similar to the crucian carp (Carassius auratus) PKR-like amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed that GrPKZ mRNA expression is at low levels in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus, GrPKZ expression was up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). Following infection with Aeromonas hydrophila, GrPKZ transcripts were induced at 24 h post-injection (P < 0.05) and returned to control levels at 120 h post-injection. These data imply that GrPKZ is involved in antiviral defense and Toll-like receptor 4 signaling pathway in bacterial infection. (C) 2008 Elsevier Ltd. All rights reserved.; Double-stranded RNA-activated protein kinase (PKR) plays an important rote in interferon-induced antiviral responses, and is also involved in intracellular signaling pathways, including the apoptosis, proliferation, and transcription pathways. In the present study, a PKR-like gene was cloned and characterized from rare minnow Gobiocypris rarus. The full length of the rare minnow PKR-like (GrPKZ) cDNA is 1946 bp in Length and encodes a polypeptide of 503 amino acids with an estimated molecular mass of 57,355 Da and a predicted isoelectric point of 5.83. Analysis of the deduced amino acid sequence indicated that the mature peptide contains two Zalpha domains and one S_TKc domain, and is most similar to the crucian carp (Carassius auratus) PKR-like amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed that GrPKZ mRNA expression is at low levels in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus, GrPKZ expression was up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). Following infection with Aeromonas hydrophila, GrPKZ transcripts were induced at 24 h post-injection (P < 0.05) and returned to control levels at 120 h post-injection. These data imply that GrPKZ is involved in antiviral defense and Toll-like receptor 4 signaling pathway in bacterial infection. (C) 2008 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordGobiocypris Rarus Z-dna Binding Protein Kinase Antiviral Gene Gene Cloning Characterization
Department[Su, Jianguo; Zhu, Zuoyan; Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Su, Jianguo] NW A&F Univ, Coll Anim Sci & Technol, Dept Aquaculture, Yangling 712100, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2008.03.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000257637600011
WOS KeywordPROTEIN-KINASE PKR ; BINDING DOMAINS ; Z-DNA ; GENOME SEQUENCE ; CAB CELLS ; RNA ; INTERFERON ; VIRUS ; REQUIREMENT ; MACROPHAGES
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8068
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.NW A&F Univ, Coll Anim Sci & Technol, Dept Aquaculture, Yangling 712100, Peoples R China
Recommended Citation
GB/T 7714
Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,et al. Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus[J]. FISH & SHELLFISH IMMUNOLOGY,2008,25(1-2):106-113.
APA Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus.FISH & SHELLFISH IMMUNOLOGY,25(1-2),106-113.
MLA Su, Jianguo,et al."Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus".FISH & SHELLFISH IMMUNOLOGY 25.1-2(2008):106-113.
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