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Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio
Mei, Jie; Chen, Bo; Yue, Huamei; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-07-16
Source PublicationMOLECULAR AND CELLULAR ENDOCRINOLOGY
ISSN0303-7207
Volume289Issue:1-2Pages:67-76
AbstractC1q family proteins with C1q domain have been reported in vertebrates, but their biological roles are currently unknown. In this study, a C1q-like factor, designated Carassius auratus gibelio ovary-specific C1q-like factor (CagOC1q-like), was identified as a cortical granules component. Immunofluorescence localization revealed that the C1q family member was specifically expressed in follicular epithelial cells, and associated with cortical granules in fully grown oocytes. Moreover, it was discharged to the perivitelline space and egg envelope upon fertilization. As it is the first identified C1q family member that is expressed in follicular cells that surround oocyte, CagOC1q-like was applied to detection of follicular cell apoptosis and deletion. The entire cytological process of follicular cell apoptosis and deletion was clearly seen from double visualizations of follicular cells with CagOC1q-like immunofluorescence and apoptotic follicular cells labeled by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) during oocyte maturation and ovulation. (C) 2008 Elsevier Ireland Ltd. All rights reserved.; C1q family proteins with C1q domain have been reported in vertebrates, but their biological roles are currently unknown. In this study, a C1q-like factor, designated Carassius auratus gibelio ovary-specific C1q-like factor (CagOC1q-like), was identified as a cortical granules component. Immunofluorescence localization revealed that the C1q family member was specifically expressed in follicular epithelial cells, and associated with cortical granules in fully grown oocytes. Moreover, it was discharged to the perivitelline space and egg envelope upon fertilization. As it is the first identified C1q family member that is expressed in follicular cells that surround oocyte, CagOC1q-like was applied to detection of follicular cell apoptosis and deletion. The entire cytological process of follicular cell apoptosis and deletion was clearly seen from double visualizations of follicular cells with CagOC1q-like immunofluorescence and apoptotic follicular cells labeled by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) during oocyte maturation and ovulation. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
SubtypeArticle
KeywordC1q Family Follicle Cortical Granule Apoptosis Ovulation Follicular Cells
Department[Mei, Jie; Chen, Bo; Yue, Huamei; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaCell Biology ; Endocrinology & Metabolism
DOI10.1016/j.mce.2008.02.016
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Endocrinology & Metabolism
WOS SubjectCell Biology ; Endocrinology & Metabolism
WOS IDWOS:000257841700009
WOS KeywordTUMOR-NECROSIS-FACTOR ; SILVER CRUCIAN CARP ; FACTOR-ALPHA ; RAT OVARY ; OOCYTE MATURATION ; GENE-EXPRESSION ; NEONATAL RAT ; IN-VITRO ; PROTEIN ; FISH
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8058
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Mei, Jie,Chen, Bo,Yue, Huamei,et al. Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2008,289(1-2):67-76.
APA Mei, Jie,Chen, Bo,Yue, Huamei,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio.MOLECULAR AND CELLULAR ENDOCRINOLOGY,289(1-2),67-76.
MLA Mei, Jie,et al."Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio".MOLECULAR AND CELLULAR ENDOCRINOLOGY 289.1-2(2008):67-76.
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