中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
学科主题: Biotechnology & Applied Microbiology; Virology
题名: Identification and characterization of a novel envelope protein in Rana grylio virus
作者: Zhao, Zhe1; Ke, Fei1; Huang, You-Hua1; Zhao, Jiu-Gang1; Gui, Jan-Fang1; Zhang, Qi-Ya1
通讯作者: Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: MAJOR CAPSID PROTEIN ; SWINE-FEVER VIRUS ; FISH CELL-LINE ; VACCINIA-VIRUS ; FAMILY IRIDOVIRIDAE ; GENE-EXPRESSION ; DNA MICROARRAYS ; VIRION ENVELOPE ; ENTRY ; MORPHOGENESIS
刊名: JOURNAL OF GENERAL VIROLOGY
发表日期: 2008-08-01
DOI: 10.1099/vir.0.2008/000810-0
卷: 89, 页:1866-1872
收录类别: SCI
文章类型: Article
部门归属: [Zhao, Zhe; Ke, Fei; Huang, You-Hua; Zhao, Jiu-Gang; Gui, Jan-Fang; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Virology
摘要: Viral envelope proteins have been proposed to play significant roles in virus infection and assembly. In this study, an envelope protein gene, 53R, was cloned and characterized from Rana grylio virus (RGV), a member of the family Iridoviridae. Database searches found its homologues in all sequenced iricloviruses, and sequence alignment revealed several conserved structural features shared by virus capsid or envelope proteins: a myristoylation site, two predicted transmembrane domains and two invariant cysteine residues. Subsequently, RT-PCR and Western blot detection revealed that the transcripts encoding RGV 53R and the protein itself appeared late during infection of fathead minnow cells and that their appearance was blocked by viral DNA replication inhibitor, indicating that RGV 53R is a late expression gene. Moreover, immunofluorescence localization found an association of 53R with virus factories in RGV-infected cells, and this association was further confirmed by expressing a 53R-GFP fusion protein in pEGFP-N3/53R-transfected cells. Furthermore, detergent extraction and Western blot detection confirmed that RGV 53R was associated with virion membrane. Therefore, the current data suggest that RGV 53R is a novel viral envelope protein and that it may play an important role in virus assembly. This is thought to be the first report on a viral envelope protein that is conserved in all sequenced iridoviruses.
英文摘要: Viral envelope proteins have been proposed to play significant roles in virus infection and assembly. In this study, an envelope protein gene, 53R, was cloned and characterized from Rana grylio virus (RGV), a member of the family Iridoviridae. Database searches found its homologues in all sequenced iricloviruses, and sequence alignment revealed several conserved structural features shared by virus capsid or envelope proteins: a myristoylation site, two predicted transmembrane domains and two invariant cysteine residues. Subsequently, RT-PCR and Western blot detection revealed that the transcripts encoding RGV 53R and the protein itself appeared late during infection of fathead minnow cells and that their appearance was blocked by viral DNA replication inhibitor, indicating that RGV 53R is a late expression gene. Moreover, immunofluorescence localization found an association of 53R with virus factories in RGV-infected cells, and this association was further confirmed by expressing a 53R-GFP fusion protein in pEGFP-N3/53R-transfected cells. Furthermore, detergent extraction and Western blot detection confirmed that RGV 53R was associated with virion membrane. Therefore, the current data suggest that RGV 53R is a novel viral envelope protein and that it may play an important role in virus assembly. This is thought to be the first report on a viral envelope protein that is conserved in all sequenced iridoviruses.
关键词[WOS]: MAJOR CAPSID PROTEIN ; SWINE-FEVER VIRUS ; FISH CELL-LINE ; VACCINIA-VIRUS ; FAMILY IRIDOVIRIDAE ; GENE-EXPRESSION ; DNA MICROARRAYS ; VIRION ENVELOPE ; ENTRY ; MORPHOGENESIS
语种: 英语
WOS记录号: WOS:000258301800009
ISSN号: 0022-1317
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8040
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Identification and characterization of a novel envelope protein in Rana grylio virus.pdf(588KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhao, Zhe; Ke, Fei; Huang, You-Hua; Zhao, Jiu-Gang; Gui, Jan-Fang; Zhang, Qi-Ya.Identification and characterization of a novel envelope protein in Rana grylio virus,JOURNAL OF GENERAL VIROLOGY,2008,89():1866-1872
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Zhe]'s Articles
[Ke, Fei]'s Articles
[Huang, You-Hua]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Zhe]‘s Articles
[Ke, Fei]‘s Articles
[Huang, You-Hua]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification and characterization of a novel envelope protein in Rana grylio virus.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace