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Identification and characterization of a novel envelope protein in Rana grylio virus
Zhao, Zhe; Ke, Fei; Huang, You-Hua; Zhao, Jiu-Gang; Gui, Jan-Fang; Zhang, Qi-Ya; Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-08-01
Source PublicationJOURNAL OF GENERAL VIROLOGY
ISSN0022-1317
Volume89Pages:1866-1872
AbstractViral envelope proteins have been proposed to play significant roles in virus infection and assembly. In this study, an envelope protein gene, 53R, was cloned and characterized from Rana grylio virus (RGV), a member of the family Iridoviridae. Database searches found its homologues in all sequenced iricloviruses, and sequence alignment revealed several conserved structural features shared by virus capsid or envelope proteins: a myristoylation site, two predicted transmembrane domains and two invariant cysteine residues. Subsequently, RT-PCR and Western blot detection revealed that the transcripts encoding RGV 53R and the protein itself appeared late during infection of fathead minnow cells and that their appearance was blocked by viral DNA replication inhibitor, indicating that RGV 53R is a late expression gene. Moreover, immunofluorescence localization found an association of 53R with virus factories in RGV-infected cells, and this association was further confirmed by expressing a 53R-GFP fusion protein in pEGFP-N3/53R-transfected cells. Furthermore, detergent extraction and Western blot detection confirmed that RGV 53R was associated with virion membrane. Therefore, the current data suggest that RGV 53R is a novel viral envelope protein and that it may play an important role in virus assembly. This is thought to be the first report on a viral envelope protein that is conserved in all sequenced iridoviruses.; Viral envelope proteins have been proposed to play significant roles in virus infection and assembly. In this study, an envelope protein gene, 53R, was cloned and characterized from Rana grylio virus (RGV), a member of the family Iridoviridae. Database searches found its homologues in all sequenced iricloviruses, and sequence alignment revealed several conserved structural features shared by virus capsid or envelope proteins: a myristoylation site, two predicted transmembrane domains and two invariant cysteine residues. Subsequently, RT-PCR and Western blot detection revealed that the transcripts encoding RGV 53R and the protein itself appeared late during infection of fathead minnow cells and that their appearance was blocked by viral DNA replication inhibitor, indicating that RGV 53R is a late expression gene. Moreover, immunofluorescence localization found an association of 53R with virus factories in RGV-infected cells, and this association was further confirmed by expressing a 53R-GFP fusion protein in pEGFP-N3/53R-transfected cells. Furthermore, detergent extraction and Western blot detection confirmed that RGV 53R was associated with virion membrane. Therefore, the current data suggest that RGV 53R is a novel viral envelope protein and that it may play an important role in virus assembly. This is thought to be the first report on a viral envelope protein that is conserved in all sequenced iridoviruses.
SubtypeArticle
KeywordMajor Capsid Protein Swine-fever Virus Fish Cell-line Vaccinia-virus Family Iridoviridae Gene-expression Dna Microarrays Virion Envelope Entry Morphogenesis
Department[Zhao, Zhe; Ke, Fei; Huang, You-Hua; Zhao, Jiu-Gang; Gui, Jan-Fang; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Virology
DOI10.1099/vir.0.2008/000810-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Virology
WOS SubjectBiotechnology & Applied Microbiology ; Virology
WOS IDWOS:000258301800009
WOS KeywordMAJOR CAPSID PROTEIN ; SWINE-FEVER VIRUS ; FISH CELL-LINE ; VACCINIA-VIRUS ; FAMILY IRIDOVIRIDAE ; GENE-EXPRESSION ; DNA MICROARRAYS ; VIRION ENVELOPE ; ENTRY ; MORPHOGENESIS
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8040
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Zhe,Ke, Fei,Huang, You-Hua,et al. Identification and characterization of a novel envelope protein in Rana grylio virus[J]. JOURNAL OF GENERAL VIROLOGY,2008,89:1866-1872.
APA Zhao, Zhe.,Ke, Fei.,Huang, You-Hua.,Zhao, Jiu-Gang.,Gui, Jan-Fang.,...&Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Identification and characterization of a novel envelope protein in Rana grylio virus.JOURNAL OF GENERAL VIROLOGY,89,1866-1872.
MLA Zhao, Zhe,et al."Identification and characterization of a novel envelope protein in Rana grylio virus".JOURNAL OF GENERAL VIROLOGY 89(2008):1866-1872.
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