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Ultrastructural alteration of lymphocytes in spleen and pronephros of grass carp (Ctenopharyngodon idella) experimentally exposed to microcystin-LR
Wei, Li Li1,2,3; Sun, Bao Jian1,2; Nie, Pin1,2; Nie, P, Chinese Acad Sci, State Key Lab Freshwater & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008-08-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume280Issue:1-4Pages:270-275
AbstractRelatively little is known in relation to pathological changes of immune organs in fish when exposed to MC-LR. The ultrastructural alteration of lymphocytes was examined in the spleen and pronephros of grass carp Ctenopharyngodon idella injected experimentally with microcystin-LR. The fish were intraperitoneally injected with MC-LR at a dose of 50 mu g/kg body weight, and the spleen and pronephros were dissected out at 1, 2, 7, 14 and 21 days post intraperitoneal injection (dpi). Pathological changes were then examined by transmission electron microscopy. Apoptosis was detected only in lymphocytes in the spleen, with obvious apoptotic features observed at 2 dpi; pathological changes of lymphocytes in the pronephros were also serious with mitochondria being highly edematous. However, damaged lymphocytes were almost un-observed in the spleen and pronephros at 21 dpi. These findings suggest that MC-LR can induce toxic effect on immune organs in grass carp, and the spleen may be much more sensitive to MC-LR stimulation. (C) 2008 Elsevier B.V. All rights reserved.; Relatively little is known in relation to pathological changes of immune organs in fish when exposed to MC-LR. The ultrastructural alteration of lymphocytes was examined in the spleen and pronephros of grass carp Ctenopharyngodon idella injected experimentally with microcystin-LR. The fish were intraperitoneally injected with MC-LR at a dose of 50 mu g/kg body weight, and the spleen and pronephros were dissected out at 1, 2, 7, 14 and 21 days post intraperitoneal injection (dpi). Pathological changes were then examined by transmission electron microscopy. Apoptosis was detected only in lymphocytes in the spleen, with obvious apoptotic features observed at 2 dpi; pathological changes of lymphocytes in the pronephros were also serious with mitochondria being highly edematous. However, damaged lymphocytes were almost un-observed in the spleen and pronephros at 21 dpi. These findings suggest that MC-LR can induce toxic effect on immune organs in grass carp, and the spleen may be much more sensitive to MC-LR stimulation. (C) 2008 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicrocystin-lr Apoptosis Mitochondrion Edema Immunotoxicity Grass Carp Spleen Pronephros
Department[Wei, Li Li; Sun, Bao Jian; Nie, Pin] Chinese Acad Sci, State Key Lab Freshwater & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Wei, Li Li; Sun, Bao Jian; Nie, Pin] Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Wei, Li Li] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1016/j.aquaculture.2008.05.015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000258965200041
WOS KeywordRAT HEPATOCYTES ; CYCLOSPORINE-A ; APOPTOSIS ; TROUT ; HEPATOTOXICITY ; ACCUMULATION ; PATHOLOGY ; TOXINS ; DEATH
Funding OrganizationNational Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8024
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Wei, Li Li,Sun, Bao Jian,Nie, Pin,et al. Ultrastructural alteration of lymphocytes in spleen and pronephros of grass carp (Ctenopharyngodon idella) experimentally exposed to microcystin-LR[J]. AQUACULTURE,2008,280(1-4):270-275.
APA Wei, Li Li,Sun, Bao Jian,Nie, Pin,&Nie, P, Chinese Acad Sci, State Key Lab Freshwater & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Ultrastructural alteration of lymphocytes in spleen and pronephros of grass carp (Ctenopharyngodon idella) experimentally exposed to microcystin-LR.AQUACULTURE,280(1-4),270-275.
MLA Wei, Li Li,et al."Ultrastructural alteration of lymphocytes in spleen and pronephros of grass carp (Ctenopharyngodon idella) experimentally exposed to microcystin-LR".AQUACULTURE 280.1-4(2008):270-275.
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