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学科主题: Immunology; Veterinary Sciences
题名: Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila
作者: Zhou, Xiuxia1, 2; Guo, Qionglin1; Dai, Heping1
通讯作者: Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: immune-relevant gene ; Chinese soft-shelled turtle ; Aeromonas hydrophila ; suppression subtractive hybridization (SSH) ; subtractive cDNA library
刊名: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
发表日期: 2008-09-15
DOI: 10.1016/j.vetimm.2008.05.008
卷: 125, 期:1-2, 页:82-91
收录类别: SCI
文章类型: Article
部门归属: [Zhou, Xiuxia; Guo, Qionglin; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Xiuxia] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091]
类目[WOS]: Immunology ; Veterinary Sciences
研究领域[WOS]: Immunology ; Veterinary Sciences
摘要: Expressed sequence tag (EST) analysis is an efficient tool for gene discovery and profiling gene expression. Aeromonas hydrophila, a ubiquitous waterborne bacterium, is one of the most frequent pathogens isolated from diseased aquatic organisms. In order to understand the molecular mechanism of anti-bacteria immune response in reptile, we have investigated the differentially expressed genes in Chinese soft-shelled turtle (Trionyx sinensis) experimentally infected with A. hydrophila by suppression subtractive hybridization (SSH). Forty-two genes were identified from more than 200 clones, of which 25 genes are found for the first time in reptiles, and classified into 6 categories: 18 in defense/immunity. 4 in catalysis, 2 in retrotransposon; 2 in cell signal transduction, 5 in cell metabolism, 10 in protein expression, and 1 in cell structure. Of the 42 differentially expressed genes, 6 genes, IL-8, serum amyloid A (SAA), CD9, CD59, activating transcription factor 4 (ATF4) and cathepsin L genes, were further observed to be up-regulated in the infected turtles by virtual Northern hybridization and RT-PCR assays. (C) 2008 Elsevier B.V. All rights reserved.
英文摘要: Expressed sequence tag (EST) analysis is an efficient tool for gene discovery and profiling gene expression. Aeromonas hydrophila, a ubiquitous waterborne bacterium, is one of the most frequent pathogens isolated from diseased aquatic organisms. In order to understand the molecular mechanism of anti-bacteria immune response in reptile, we have investigated the differentially expressed genes in Chinese soft-shelled turtle (Trionyx sinensis) experimentally infected with A. hydrophila by suppression subtractive hybridization (SSH). Forty-two genes were identified from more than 200 clones, of which 25 genes are found for the first time in reptiles, and classified into 6 categories: 18 in defense/immunity. 4 in catalysis, 2 in retrotransposon; 2 in cell signal transduction, 5 in cell metabolism, 10 in protein expression, and 1 in cell structure. Of the 42 differentially expressed genes, 6 genes, IL-8, serum amyloid A (SAA), CD9, CD59, activating transcription factor 4 (ATF4) and cathepsin L genes, were further observed to be up-regulated in the infected turtles by virtual Northern hybridization and RT-PCR assays. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: PHYLOGENETICALLY ANCIENT REPTILE ; UNFOLDED PROTEIN RESPONSE ; ENDOPLASMIC-RETICULUM ; ENDOTHELIAL-CELLS ; VIRULENCE FACTORS ; IMMUNOGLOBULIN ; MHC ; TUATARA ; CLONING ; CAIMAN
语种: 英语
WOS记录号: WOS:000259313300008
ISSN号: 0165-2427
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8004
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhou, Xiuxia; Guo, Qionglin; Dai, Heping.Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila,VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2008,125(1-2):82-91
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