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Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila
Zhou, Xiuxia1,2; Guo, Qionglin1; Dai, Heping1; Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008-09-15
Source PublicationVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN0165-2427
Volume125Issue:1-2Pages:82-91
AbstractExpressed sequence tag (EST) analysis is an efficient tool for gene discovery and profiling gene expression. Aeromonas hydrophila, a ubiquitous waterborne bacterium, is one of the most frequent pathogens isolated from diseased aquatic organisms. In order to understand the molecular mechanism of anti-bacteria immune response in reptile, we have investigated the differentially expressed genes in Chinese soft-shelled turtle (Trionyx sinensis) experimentally infected with A. hydrophila by suppression subtractive hybridization (SSH). Forty-two genes were identified from more than 200 clones, of which 25 genes are found for the first time in reptiles, and classified into 6 categories: 18 in defense/immunity. 4 in catalysis, 2 in retrotransposon; 2 in cell signal transduction, 5 in cell metabolism, 10 in protein expression, and 1 in cell structure. Of the 42 differentially expressed genes, 6 genes, IL-8, serum amyloid A (SAA), CD9, CD59, activating transcription factor 4 (ATF4) and cathepsin L genes, were further observed to be up-regulated in the infected turtles by virtual Northern hybridization and RT-PCR assays. (C) 2008 Elsevier B.V. All rights reserved.; Expressed sequence tag (EST) analysis is an efficient tool for gene discovery and profiling gene expression. Aeromonas hydrophila, a ubiquitous waterborne bacterium, is one of the most frequent pathogens isolated from diseased aquatic organisms. In order to understand the molecular mechanism of anti-bacteria immune response in reptile, we have investigated the differentially expressed genes in Chinese soft-shelled turtle (Trionyx sinensis) experimentally infected with A. hydrophila by suppression subtractive hybridization (SSH). Forty-two genes were identified from more than 200 clones, of which 25 genes are found for the first time in reptiles, and classified into 6 categories: 18 in defense/immunity. 4 in catalysis, 2 in retrotransposon; 2 in cell signal transduction, 5 in cell metabolism, 10 in protein expression, and 1 in cell structure. Of the 42 differentially expressed genes, 6 genes, IL-8, serum amyloid A (SAA), CD9, CD59, activating transcription factor 4 (ATF4) and cathepsin L genes, were further observed to be up-regulated in the infected turtles by virtual Northern hybridization and RT-PCR assays. (C) 2008 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordImmune-relevant Gene Chinese Soft-shelled Turtle Aeromonas Hydrophila Suppression Subtractive Hybridization (Ssh) Subtractive Cdna Library
Department[Zhou, Xiuxia; Guo, Qionglin; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Xiuxia] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaImmunology ; Veterinary Sciences
DOI10.1016/j.vetimm.2008.05.008
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091] ; National Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091] ; National Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091] ; National Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Veterinary Sciences
WOS SubjectImmunology ; Veterinary Sciences
WOS IDWOS:000259313300008
WOS KeywordPHYLOGENETICALLY ANCIENT REPTILE ; UNFOLDED PROTEIN RESPONSE ; ENDOPLASMIC-RETICULUM ; ENDOTHELIAL-CELLS ; VIRULENCE FACTORS ; IMMUNOGLOBULIN ; MHC ; TUATARA ; CLONING ; CAIMAN
Funding OrganizationNational Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091] ; National Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091] ; National Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091] ; National Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091]
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Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8004
Collection期刊论文
Corresponding AuthorGuo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Xiuxia,Guo, Qionglin,Dai, Heping,et al. Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2008,125(1-2):82-91.
APA Zhou, Xiuxia,Guo, Qionglin,Dai, Heping,&Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,125(1-2),82-91.
MLA Zhou, Xiuxia,et al."Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 125.1-2(2008):82-91.
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