| Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell | |
| Zhang, Xiaoling1,2; Yang, Fangxing1; Xu, Chao3; Liu, Weiping3; Wen, Sheng1,2; Xu, Ying1,2; Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China | |
| 2008-09-01 | |
| Source Publication | TOXICOLOGY IN VITRO
 |
| ISSN | 0887-2333 |
| Volume | 22Issue:6Pages:1520-1527 |
| Abstract | Hexabromocyclododecanes (HBCDs) are additive brominated flame retardants mainly used in plastics and textiles. At the present time, these compounds are found in almost all environmental and human samples. In order to evaluate the environmental safety and health risk of HBCDs, the enantiomerically pure alpha-, beta-, and gamma-HBCD were prepared using high performance liquid chromatography (HPLC) on a PM-P-CD column and the cytotoxicities of their enantiomers were evaluated in Hep G2 cells. Results from the 3-(4,5-dimethylthioazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), resazurin reduction and lactate dehydrogenase (LDH) release assays showed a good agreement that the order of cytotoxicity was gamma-HBCD >= beta-HBCD > alpha-HBCD, and that significantly lower cell viability and higher LDH release were observed in all (+)-enantiomers ((+) alpha-, (+) beta- and (+) gamma-HBCD) than the corresponding (-)-forms ((-) alpha-, (-) beta- and (-) gamma-HBCD). Additionally, the formation of reactive oxygen species (ROS) induced by these HBCD enantiomers were detected. The positive correlation between the LDH release and ROS formation demonstrated that the toxic mechanism might be mediated by oxidative damage. These results suggest that environmental and human health risks of HBCDs must be evaluated at the level of individual enantiomers. (C) 2008 Published by Elsevier Ltd.; Hexabromocyclododecanes (HBCDs) are additive brominated flame retardants mainly used in plastics and textiles. At the present time, these compounds are found in almost all environmental and human samples. In order to evaluate the environmental safety and health risk of HBCDs, the enantiomerically pure alpha-, beta-, and gamma-HBCD were prepared using high performance liquid chromatography (HPLC) on a PM-P-CD column and the cytotoxicities of their enantiomers were evaluated in Hep G2 cells. Results from the 3-(4,5-dimethylthioazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), resazurin reduction and lactate dehydrogenase (LDH) release assays showed a good agreement that the order of cytotoxicity was gamma-HBCD >= beta-HBCD > alpha-HBCD, and that significantly lower cell viability and higher LDH release were observed in all (+)-enantiomers ((+) alpha-, (+) beta- and (+) gamma-HBCD) than the corresponding (-)-forms ((-) alpha-, (-) beta- and (-) gamma-HBCD). Additionally, the formation of reactive oxygen species (ROS) induced by these HBCD enantiomers were detected. The positive correlation between the LDH release and ROS formation demonstrated that the toxic mechanism might be mediated by oxidative damage. These results suggest that environmental and human health risks of HBCDs must be evaluated at the level of individual enantiomers. (C) 2008 Published by Elsevier Ltd. |
| Subtype | Article |
| Keyword | Hexabromocyclododecanes Cytotoxicity Reactive Oxygen Species Diastereomers Enantiomers |
| Department | [Zhang, Xiaoling; Yang, Fangxing; Wen, Sheng; Xu, Ying] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Zhang, Xiaoling; Wen, Sheng; Xu, Ying] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xu, Chao; Liu, Weiping] Zhejiang Univ Technol, Coll Biol & Environm Engn, Res Ctr Environm Sci, Hangzhou 310032, Zhejiang, Peoples R China |
| Subject Area | Toxicology |
| DOI | 10.1016/j.tiv.2008.05.006 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Toxicology |
| WOS Subject | Toxicology |
| WOS ID | WOS:000259547300014 |
| WOS Keyword | BROMINATED FLAME RETARDANTS ; POLYBROMINATED DIPHENYL ETHERS ; ADVANCED GLYCATION ENDPRODUCTS ; CULTURED CORTICAL-NEURONS ; IN-VITRO ; HEPATOMA-CELL ; TETRABROMOBISPHENOL-A ; CADMIUM CHLORIDE ; ALAMAR BLUE ; APOPTOSIS |
| Funding Organization | 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7998 |
| Collection | 期刊论文 |
| Corresponding Author | Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Zhejiang Univ Technol, Coll Biol & Environm Engn, Res Ctr Environm Sci, Hangzhou 310032, Zhejiang, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Xiaoling,Yang, Fangxing,Xu, Chao,et al. Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell[J]. TOXICOLOGY IN VITRO,2008,22(6):1520-1527. |
| APA | Zhang, Xiaoling.,Yang, Fangxing.,Xu, Chao.,Liu, Weiping.,Wen, Sheng.,...&Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China.(2008).Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell.TOXICOLOGY IN VITRO,22(6),1520-1527. |
| MLA | Zhang, Xiaoling,et al."Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell".TOXICOLOGY IN VITRO 22.6(2008):1520-1527. |
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