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学科主题: Toxicology
题名: Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell
作者: Zhang, Xiaoling1, 2; Yang, Fangxing1; Xu, Chao3; Liu, Weiping3; Wen, Sheng1, 2; Xu, Ying1, 2
通讯作者: Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China
关键词: hexabromocyclododecanes ; cytotoxicity ; reactive oxygen species ; diastereomers ; enantiomers
刊名: TOXICOLOGY IN VITRO
发表日期: 2008-09-01
DOI: 10.1016/j.tiv.2008.05.006
卷: 22, 期:6, 页:1520-1527
收录类别: SCI
文章类型: Article
部门归属: [Zhang, Xiaoling; Yang, Fangxing; Wen, Sheng; Xu, Ying] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Zhang, Xiaoling; Wen, Sheng; Xu, Ying] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xu, Chao; Liu, Weiping] Zhejiang Univ Technol, Coll Biol & Environm Engn, Res Ctr Environm Sci, Hangzhou 310032, Zhejiang, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005]
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
摘要: Hexabromocyclododecanes (HBCDs) are additive brominated flame retardants mainly used in plastics and textiles. At the present time, these compounds are found in almost all environmental and human samples. In order to evaluate the environmental safety and health risk of HBCDs, the enantiomerically pure alpha-, beta-, and gamma-HBCD were prepared using high performance liquid chromatography (HPLC) on a PM-P-CD column and the cytotoxicities of their enantiomers were evaluated in Hep G2 cells. Results from the 3-(4,5-dimethylthioazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), resazurin reduction and lactate dehydrogenase (LDH) release assays showed a good agreement that the order of cytotoxicity was gamma-HBCD >= beta-HBCD > alpha-HBCD, and that significantly lower cell viability and higher LDH release were observed in all (+)-enantiomers ((+) alpha-, (+) beta- and (+) gamma-HBCD) than the corresponding (-)-forms ((-) alpha-, (-) beta- and (-) gamma-HBCD). Additionally, the formation of reactive oxygen species (ROS) induced by these HBCD enantiomers were detected. The positive correlation between the LDH release and ROS formation demonstrated that the toxic mechanism might be mediated by oxidative damage. These results suggest that environmental and human health risks of HBCDs must be evaluated at the level of individual enantiomers. (C) 2008 Published by Elsevier Ltd.
英文摘要: Hexabromocyclododecanes (HBCDs) are additive brominated flame retardants mainly used in plastics and textiles. At the present time, these compounds are found in almost all environmental and human samples. In order to evaluate the environmental safety and health risk of HBCDs, the enantiomerically pure alpha-, beta-, and gamma-HBCD were prepared using high performance liquid chromatography (HPLC) on a PM-P-CD column and the cytotoxicities of their enantiomers were evaluated in Hep G2 cells. Results from the 3-(4,5-dimethylthioazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), resazurin reduction and lactate dehydrogenase (LDH) release assays showed a good agreement that the order of cytotoxicity was gamma-HBCD >= beta-HBCD > alpha-HBCD, and that significantly lower cell viability and higher LDH release were observed in all (+)-enantiomers ((+) alpha-, (+) beta- and (+) gamma-HBCD) than the corresponding (-)-forms ((-) alpha-, (-) beta- and (-) gamma-HBCD). Additionally, the formation of reactive oxygen species (ROS) induced by these HBCD enantiomers were detected. The positive correlation between the LDH release and ROS formation demonstrated that the toxic mechanism might be mediated by oxidative damage. These results suggest that environmental and human health risks of HBCDs must be evaluated at the level of individual enantiomers. (C) 2008 Published by Elsevier Ltd.
关键词[WOS]: BROMINATED FLAME RETARDANTS ; POLYBROMINATED DIPHENYL ETHERS ; ADVANCED GLYCATION ENDPRODUCTS ; CULTURED CORTICAL-NEURONS ; IN-VITRO ; HEPATOMA-CELL ; TETRABROMOBISPHENOL-A ; CADMIUM CHLORIDE ; ALAMAR BLUE ; APOPTOSIS
语种: 英语
WOS记录号: WOS:000259547300014
ISSN号: 0887-2333
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7998
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Zhejiang Univ Technol, Coll Biol & Environm Engn, Res Ctr Environm Sci, Hangzhou 310032, Zhejiang, Peoples R China

Recommended Citation:
Zhang, Xiaoling; Yang, Fangxing; Xu, Chao; Liu, Weiping; Wen, Sheng; Xu, Ying.Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell,TOXICOLOGY IN VITRO,2008,22(6):1520-1527
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