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Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell
Zhang, Xiaoling1,2; Yang, Fangxing1; Xu, Chao3; Liu, Weiping3; Wen, Sheng1,2; Xu, Ying1,2; Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China
2008-09-01
Source PublicationTOXICOLOGY IN VITRO
ISSN0887-2333
Volume22Issue:6Pages:1520-1527
AbstractHexabromocyclododecanes (HBCDs) are additive brominated flame retardants mainly used in plastics and textiles. At the present time, these compounds are found in almost all environmental and human samples. In order to evaluate the environmental safety and health risk of HBCDs, the enantiomerically pure alpha-, beta-, and gamma-HBCD were prepared using high performance liquid chromatography (HPLC) on a PM-P-CD column and the cytotoxicities of their enantiomers were evaluated in Hep G2 cells. Results from the 3-(4,5-dimethylthioazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), resazurin reduction and lactate dehydrogenase (LDH) release assays showed a good agreement that the order of cytotoxicity was gamma-HBCD >= beta-HBCD > alpha-HBCD, and that significantly lower cell viability and higher LDH release were observed in all (+)-enantiomers ((+) alpha-, (+) beta- and (+) gamma-HBCD) than the corresponding (-)-forms ((-) alpha-, (-) beta- and (-) gamma-HBCD). Additionally, the formation of reactive oxygen species (ROS) induced by these HBCD enantiomers were detected. The positive correlation between the LDH release and ROS formation demonstrated that the toxic mechanism might be mediated by oxidative damage. These results suggest that environmental and human health risks of HBCDs must be evaluated at the level of individual enantiomers. (C) 2008 Published by Elsevier Ltd.; Hexabromocyclododecanes (HBCDs) are additive brominated flame retardants mainly used in plastics and textiles. At the present time, these compounds are found in almost all environmental and human samples. In order to evaluate the environmental safety and health risk of HBCDs, the enantiomerically pure alpha-, beta-, and gamma-HBCD were prepared using high performance liquid chromatography (HPLC) on a PM-P-CD column and the cytotoxicities of their enantiomers were evaluated in Hep G2 cells. Results from the 3-(4,5-dimethylthioazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), resazurin reduction and lactate dehydrogenase (LDH) release assays showed a good agreement that the order of cytotoxicity was gamma-HBCD >= beta-HBCD > alpha-HBCD, and that significantly lower cell viability and higher LDH release were observed in all (+)-enantiomers ((+) alpha-, (+) beta- and (+) gamma-HBCD) than the corresponding (-)-forms ((-) alpha-, (-) beta- and (-) gamma-HBCD). Additionally, the formation of reactive oxygen species (ROS) induced by these HBCD enantiomers were detected. The positive correlation between the LDH release and ROS formation demonstrated that the toxic mechanism might be mediated by oxidative damage. These results suggest that environmental and human health risks of HBCDs must be evaluated at the level of individual enantiomers. (C) 2008 Published by Elsevier Ltd.
SubtypeArticle
KeywordHexabromocyclododecanes Cytotoxicity Reactive Oxygen Species Diastereomers Enantiomers
Department[Zhang, Xiaoling; Yang, Fangxing; Wen, Sheng; Xu, Ying] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Zhang, Xiaoling; Wen, Sheng; Xu, Ying] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xu, Chao; Liu, Weiping] Zhejiang Univ Technol, Coll Biol & Environm Engn, Res Ctr Environm Sci, Hangzhou 310032, Zhejiang, Peoples R China
Subject AreaToxicology
DOI10.1016/j.tiv.2008.05.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005]
Indexed BySCI
Language英语
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000259547300014
WOS KeywordBROMINATED FLAME RETARDANTS ; POLYBROMINATED DIPHENYL ETHERS ; ADVANCED GLYCATION ENDPRODUCTS ; CULTURED CORTICAL-NEURONS ; IN-VITRO ; HEPATOMA-CELL ; TETRABROMOBISPHENOL-A ; CADMIUM CHLORIDE ; ALAMAR BLUE ; APOPTOSIS
Funding Organization863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005] ; 863 Research Project [2006AA-06Z424]; Ministry of Science and Technology, China [2003CB415005]
Citation statistics
Cited Times:60[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7998
Collection期刊论文
Corresponding AuthorXu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Zhejiang Univ Technol, Coll Biol & Environm Engn, Res Ctr Environm Sci, Hangzhou 310032, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xiaoling,Yang, Fangxing,Xu, Chao,et al. Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell[J]. TOXICOLOGY IN VITRO,2008,22(6):1520-1527.
APA Zhang, Xiaoling.,Yang, Fangxing.,Xu, Chao.,Liu, Weiping.,Wen, Sheng.,...&Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China.(2008).Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell.TOXICOLOGY IN VITRO,22(6),1520-1527.
MLA Zhang, Xiaoling,et al."Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell".TOXICOLOGY IN VITRO 22.6(2008):1520-1527.
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