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Cloning, characterization, and gene expression analysis of a novel cadmium metallothionein gene in Tetrahymena pigmentosa
Guo, Lina1,2; Fu, Chengjie1,2; Miao, Wei1; Miao, W, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China
2008-10-15
Source PublicationGENE
ISSN0378-1119
Volume423Issue:1Pages:29-35
AbstractA novel cadmium-inducible metallothionein (MT) gene (Tpig-MT1) was cloned and sequenced from the ciliate Tetrahymena pigmentosa. The number of deduced amino acids is 118. The polypeptide possesses CCC and CC clusters characteristic of typical Tetrahymena Cd-inducible MTs. The structure of Tpig-MT1 is different from the reported Cd-MT in T. pyriformis, T. thermophila and T. pigmentosa. Tpig-MT1 contains two intragenic tandem repeats with 72.9% identity described as Tpig-MT1 (repeat A1) and Tpig-MT1 (repeat A2). The transcriptional response of Tpig-MT1 gene to different heavy metals (Cd, Cu, Zn, Hg, Pb) and oxidative stress (H2O2) was measured using real-time quantitative PCR. The results showed that the gene was quickly induced (1 h) by the five heavy metals and the order of expression level was Hg>Pb>Cd>Cu>Zn. The induction effect of H2O2 was 5-fold after about 15 min, but soon decreased to a non-significant level (30 min). The genetic diversity of Tetrahymena MT genes is discussed in relation to the unique structure of the Tpig-MT1 gene and other reported Cd-MT isoforms. (C) 2008 Elsevier B.V. All rights reserved.; A novel cadmium-inducible metallothionein (MT) gene (Tpig-MT1) was cloned and sequenced from the ciliate Tetrahymena pigmentosa. The number of deduced amino acids is 118. The polypeptide possesses CCC and CC clusters characteristic of typical Tetrahymena Cd-inducible MTs. The structure of Tpig-MT1 is different from the reported Cd-MT in T. pyriformis, T. thermophila and T. pigmentosa. Tpig-MT1 contains two intragenic tandem repeats with 72.9% identity described as Tpig-MT1 (repeat A1) and Tpig-MT1 (repeat A2). The transcriptional response of Tpig-MT1 gene to different heavy metals (Cd, Cu, Zn, Hg, Pb) and oxidative stress (H2O2) was measured using real-time quantitative PCR. The results showed that the gene was quickly induced (1 h) by the five heavy metals and the order of expression level was Hg>Pb>Cd>Cu>Zn. The induction effect of H2O2 was 5-fold after about 15 min, but soon decreased to a non-significant level (30 min). The genetic diversity of Tetrahymena MT genes is discussed in relation to the unique structure of the Tpig-MT1 gene and other reported Cd-MT isoforms. (C) 2008 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordGene Duplication Genetic Diversity Heavy Metals Internal Similarity Real-time Quantitative Pcr Oxidative Stress
Department[Guo, Lina; Fu, Chengjie; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Guo, Lina; Fu, Chengjie] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaGenetics & Heredity
DOI10.1016/j.gene.2008.04.023
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30670298]; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2006DFA31960] ; National Natural Science Foundation of China [30670298]; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2006DFA31960] ; National Natural Science Foundation of China [30670298]; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2006DFA31960] ; National Natural Science Foundation of China [30670298]; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2006DFA31960]
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000259943700005
WOS KeywordSTRESS-INDUCIBLE METALLOTHIONEIN ; DIFFERENTIAL EXPRESSION ; PROTEIN ; THERMOPHILA ; INDUCTION ; PYRIFORMIS ; MT ; IDENTIFICATION ; RATS ; LEAD
Funding OrganizationNational Natural Science Foundation of China [30670298]; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2006DFA31960] ; National Natural Science Foundation of China [30670298]; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2006DFA31960] ; National Natural Science Foundation of China [30670298]; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2006DFA31960] ; National Natural Science Foundation of China [30670298]; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2006DFA31960]
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Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7992
Collection期刊论文
Corresponding AuthorMiao, W, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Guo, Lina,Fu, Chengjie,Miao, Wei,et al. Cloning, characterization, and gene expression analysis of a novel cadmium metallothionein gene in Tetrahymena pigmentosa[J]. GENE,2008,423(1):29-35.
APA Guo, Lina,Fu, Chengjie,Miao, Wei,&Miao, W, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China.(2008).Cloning, characterization, and gene expression analysis of a novel cadmium metallothionein gene in Tetrahymena pigmentosa.GENE,423(1),29-35.
MLA Guo, Lina,et al."Cloning, characterization, and gene expression analysis of a novel cadmium metallothionein gene in Tetrahymena pigmentosa".GENE 423.1(2008):29-35.
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