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学科主题: Environmental Sciences
题名: Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater
作者: Du Cheng1, 2; Wu Zhenbin1; Xiao Enrong1, 2; Zhou Qiaohong1; Cheng Shuiping1; Liang Wei1; He Feng1
通讯作者: Wu, ZB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: bacterial diversity ; restriction fragment length polymorphism (RFLP) ; submerged membrane bioreactor (SMBR) ; 16S rDNA clone library
刊名: JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
发表日期: 2008
卷: 20, 期:10, 页:1210-1217
收录类别: SCI
文章类型: Article
部门归属: [Du Cheng; Wu Zhenbin; Xiao Enrong; Zhou Qiaohong; Cheng Shuiping; Liang Wei; He Feng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Du Cheng; Xiao Enrong] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102]
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
摘要: The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCB1 for identification purposes. The 90.6% of the clones were affiliated with the two phyla Bacteroidetes (50%) and Proteobacteria (40%), and beta-, -gamma-, and delta-Proteobacteria accounted for 7.8%, 28.1%, and 4.7%, respectively. Minor portions were affiliated with the Actinobacteria and Firmicutes (both 3.1%). Only 6 out of 64 16S rDNA sequences exhibited similarities of more than 97% to classified bacterial species, which indicated that a substantial fraction of the clone sequences were derived from unknown taxa. Rarefaction analysis of operational taxonomic units (orrUs) clusters demonstrated that 150 clones screened were still insufficient to describe the whole bacterial diversity. Measurement of water quality parameter demonstrated that performance of the SMBR maintained high level, and the SMBR system remained stable during this study.
英文摘要: The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCB1 for identification purposes. The 90.6% of the clones were affiliated with the two phyla Bacteroidetes (50%) and Proteobacteria (40%), and beta-, -gamma-, and delta-Proteobacteria accounted for 7.8%, 28.1%, and 4.7%, respectively. Minor portions were affiliated with the Actinobacteria and Firmicutes (both 3.1%). Only 6 out of 64 16S rDNA sequences exhibited similarities of more than 97% to classified bacterial species, which indicated that a substantial fraction of the clone sequences were derived from unknown taxa. Rarefaction analysis of operational taxonomic units (orrUs) clusters demonstrated that 150 clones screened were still insufficient to describe the whole bacterial diversity. Measurement of water quality parameter demonstrated that performance of the SMBR maintained high level, and the SMBR system remained stable during this study.
关键词[WOS]: MICROBIAL DIVERSITY ; NITROGEN REMOVAL ; 16S RDNA ; COMMUNITY ; IDENTIFICATION ; POPULATIONS ; SEDIMENTS ; GENUS ; SOIL
语种: 英语
WOS记录号: WOS:000260099600009
ISSN号: 1001-0742
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7982
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China

Recommended Citation:
Du Cheng; Wu Zhenbin; Xiao Enrong; Zhou Qiaohong; Cheng Shuiping; Liang Wei; He Feng.Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater,JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(10):1210-1217
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