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Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater
Du Cheng1,2; Wu Zhenbin1; Xiao Enrong1,2; Zhou Qiaohong1; Cheng Shuiping1; Liang Wei1; He Feng1; Wu, ZB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008
Source PublicationJOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
ISSN1001-0742
Volume20Issue:10Pages:1210-1217
AbstractThe bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCB1 for identification purposes. The 90.6% of the clones were affiliated with the two phyla Bacteroidetes (50%) and Proteobacteria (40%), and beta-, -gamma-, and delta-Proteobacteria accounted for 7.8%, 28.1%, and 4.7%, respectively. Minor portions were affiliated with the Actinobacteria and Firmicutes (both 3.1%). Only 6 out of 64 16S rDNA sequences exhibited similarities of more than 97% to classified bacterial species, which indicated that a substantial fraction of the clone sequences were derived from unknown taxa. Rarefaction analysis of operational taxonomic units (orrUs) clusters demonstrated that 150 clones screened were still insufficient to describe the whole bacterial diversity. Measurement of water quality parameter demonstrated that performance of the SMBR maintained high level, and the SMBR system remained stable during this study.; The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCB1 for identification purposes. The 90.6% of the clones were affiliated with the two phyla Bacteroidetes (50%) and Proteobacteria (40%), and beta-, -gamma-, and delta-Proteobacteria accounted for 7.8%, 28.1%, and 4.7%, respectively. Minor portions were affiliated with the Actinobacteria and Firmicutes (both 3.1%). Only 6 out of 64 16S rDNA sequences exhibited similarities of more than 97% to classified bacterial species, which indicated that a substantial fraction of the clone sequences were derived from unknown taxa. Rarefaction analysis of operational taxonomic units (orrUs) clusters demonstrated that 150 clones screened were still insufficient to describe the whole bacterial diversity. Measurement of water quality parameter demonstrated that performance of the SMBR maintained high level, and the SMBR system remained stable during this study.
SubtypeArticle
KeywordBacterial Diversity Restriction Fragment Length Polymorphism (Rflp) Submerged Membrane Bioreactor (Smbr) 16s Rdna Clone Library
Department[Du Cheng; Wu Zhenbin; Xiao Enrong; Zhou Qiaohong; Cheng Shuiping; Liang Wei; He Feng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Du Cheng; Xiao Enrong] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Subject AreaEnvironmental Sciences
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000260099600009
WOS KeywordMICROBIAL DIVERSITY ; NITROGEN REMOVAL ; 16S RDNA ; COMMUNITY ; IDENTIFICATION ; POPULATIONS ; SEDIMENTS ; GENUS ; SOIL
Funding OrganizationNational Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102]
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7982
Collection期刊论文
Corresponding AuthorWu, ZB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Du Cheng,Wu Zhenbin,Xiao Enrong,et al. Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(10):1210-1217.
APA Du Cheng.,Wu Zhenbin.,Xiao Enrong.,Zhou Qiaohong.,Cheng Shuiping.,...&Wu, ZB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,20(10),1210-1217.
MLA Du Cheng,et al."Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 20.10(2008):1210-1217.
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