| Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater | |
| Du Cheng1,2; Wu Zhenbin1; Xiao Enrong1,2; Zhou Qiaohong1; Cheng Shuiping1; Liang Wei1; He Feng1; Wu, ZB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2008 | |
| Source Publication | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| ISSN | 1001-0742 |
| Volume | 20Issue:10Pages:1210-1217 |
| Abstract | The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCB1 for identification purposes. The 90.6% of the clones were affiliated with the two phyla Bacteroidetes (50%) and Proteobacteria (40%), and beta-, -gamma-, and delta-Proteobacteria accounted for 7.8%, 28.1%, and 4.7%, respectively. Minor portions were affiliated with the Actinobacteria and Firmicutes (both 3.1%). Only 6 out of 64 16S rDNA sequences exhibited similarities of more than 97% to classified bacterial species, which indicated that a substantial fraction of the clone sequences were derived from unknown taxa. Rarefaction analysis of operational taxonomic units (orrUs) clusters demonstrated that 150 clones screened were still insufficient to describe the whole bacterial diversity. Measurement of water quality parameter demonstrated that performance of the SMBR maintained high level, and the SMBR system remained stable during this study.; The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCB1 for identification purposes. The 90.6% of the clones were affiliated with the two phyla Bacteroidetes (50%) and Proteobacteria (40%), and beta-, -gamma-, and delta-Proteobacteria accounted for 7.8%, 28.1%, and 4.7%, respectively. Minor portions were affiliated with the Actinobacteria and Firmicutes (both 3.1%). Only 6 out of 64 16S rDNA sequences exhibited similarities of more than 97% to classified bacterial species, which indicated that a substantial fraction of the clone sequences were derived from unknown taxa. Rarefaction analysis of operational taxonomic units (orrUs) clusters demonstrated that 150 clones screened were still insufficient to describe the whole bacterial diversity. Measurement of water quality parameter demonstrated that performance of the SMBR maintained high level, and the SMBR system remained stable during this study. |
| Subtype | Article |
| Keyword | Bacterial Diversity Restriction Fragment Length Polymorphism (Rflp) Submerged Membrane Bioreactor (Smbr) 16s Rdna Clone Library |
| Department | [Du Cheng; Wu Zhenbin; Xiao Enrong; Zhou Qiaohong; Cheng Shuiping; Liang Wei; He Feng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Du Cheng; Xiao Enrong] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| Subject Area | Environmental Sciences |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Environmental Sciences & Ecology |
| WOS Subject | Environmental Sciences |
| WOS ID | WOS:000260099600009 |
| WOS Keyword | MICROBIAL DIVERSITY ; NITROGEN REMOVAL ; 16S RDNA ; COMMUNITY ; IDENTIFICATION ; POPULATIONS ; SEDIMENTS ; GENUS ; SOIL |
| Funding Organization | National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] ; National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7982 |
| Collection | 期刊论文 |
| Corresponding Author | Wu, ZB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| Recommended Citation GB/T 7714 | Du Cheng,Wu Zhenbin,Xiao Enrong,et al. Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(10):1210-1217. |
| APA | Du Cheng.,Wu Zhenbin.,Xiao Enrong.,Zhou Qiaohong.,Cheng Shuiping.,...&Wu, ZB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,20(10),1210-1217. |
| MLA | Du Cheng,et al."Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 20.10(2008):1210-1217. |
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