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Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio
Guo, Wei1,2,3; Gui, Jian-Fang1; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-12-01
Source PublicationAQUACULTURE INTERNATIONAL
ISSN0967-6120
Volume16Issue:6Pages:497-510
AbstractMicrosatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.; Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.
SubtypeArticle
KeywordGibel Carp Microsatellite Gynogenesis Polyploidy Molecular Marker
Department[Guo, Wei; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Guo, Wei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Guo, Wei] Fuzhou Univ, Inst Biol & Med Technol, Fuzhou 350001, Peoples R China
Subject AreaFisheries
DOI10.1007/s10499-007-9161-7
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206]
Indexed BySCI
Language英语
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000260368100002
WOS KeywordSILVER CRUCIAN CARP ; MOLECULAR MARKERS ; BLOCH ; DNA ; CLONES ; SEQUENCES ; ORIGIN ; GENOME ; LOCI
Funding OrganizationNational Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206]
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7980
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Fuzhou Univ, Inst Biol & Med Technol, Fuzhou 350001, Peoples R China
Recommended Citation
GB/T 7714
Guo, Wei,Gui, Jian-Fang,Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio[J]. AQUACULTURE INTERNATIONAL,2008,16(6):497-510.
APA Guo, Wei,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio.AQUACULTURE INTERNATIONAL,16(6),497-510.
MLA Guo, Wei,et al."Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio".AQUACULTURE INTERNATIONAL 16.6(2008):497-510.
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