| Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio | |
| Guo, Wei1,2,3; Gui, Jian-Fang1; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2008-12-01 | |
| Source Publication | AQUACULTURE INTERNATIONAL
 |
| ISSN | 0967-6120 |
| Volume | 16Issue:6Pages:497-510 |
| Abstract | Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.; Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp. |
| Subtype | Article |
| Keyword | Gibel Carp Microsatellite Gynogenesis Polyploidy Molecular Marker |
| Department | [Guo, Wei; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Guo, Wei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Guo, Wei] Fuzhou Univ, Inst Biol & Med Technol, Fuzhou 350001, Peoples R China |
| Subject Area | Fisheries |
| DOI | 10.1007/s10499-007-9161-7 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries |
| WOS Subject | Fisheries |
| WOS ID | WOS:000260368100002 |
| WOS Keyword | SILVER CRUCIAN CARP ; MOLECULAR MARKERS ; BLOCH ; DNA ; CLONES ; SEQUENCES ; ORIGIN ; GENOME ; LOCI |
| Funding Organization | National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] ; National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7980 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Fuzhou Univ, Inst Biol & Med Technol, Fuzhou 350001, Peoples R China |
| Recommended Citation GB/T 7714 | Guo, Wei,Gui, Jian-Fang,Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio[J]. AQUACULTURE INTERNATIONAL,2008,16(6):497-510. |
| APA | Guo, Wei,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio.AQUACULTURE INTERNATIONAL,16(6),497-510. |
| MLA | Guo, Wei,et al."Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio".AQUACULTURE INTERNATIONAL 16.6(2008):497-510. |
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