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学科主题: Fisheries
题名: Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio
作者: Guo, Wei1, 2, 3; Gui, Jian-Fang1
通讯作者: Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Gibel carp ; Microsatellite ; Gynogenesis ; Polyploidy ; Molecular marker
刊名: AQUACULTURE INTERNATIONAL
发表日期: 2008-12-01
DOI: 10.1007/s10499-007-9161-7
卷: 16, 期:6, 页:497-510
收录类别: SCI
文章类型: Article
部门归属: [Guo, Wei; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Guo, Wei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Guo, Wei] Fuzhou Univ, Inst Biol & Med Technol, Fuzhou 350001, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206]
类目[WOS]: Fisheries
研究领域[WOS]: Fisheries
摘要: Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.
英文摘要: Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.
关键词[WOS]: SILVER CRUCIAN CARP ; MOLECULAR MARKERS ; BLOCH ; DNA ; CLONES ; SEQUENCES ; ORIGIN ; GENOME ; LOCI
语种: 英语
WOS记录号: WOS:000260368100002
ISSN号: 0967-6120
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7980
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Fuzhou Univ, Inst Biol & Med Technol, Fuzhou 350001, Peoples R China

Recommended Citation:
Guo, Wei; Gui, Jian-Fang.Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio,AQUACULTURE INTERNATIONAL,2008,16(6):497-510
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