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Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis
Qi, Z. T.1,2,3; Nie, P.1,2; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2008-11-01
Source PublicationIMMUNOGENETICS
ISSN0093-7711
Volume60Issue:11Pages:699-710
AbstractUsing bioinformatics approach, the genome locus containing interleukin (IL)-22, IL-26, and interferon gamma (IFN-gamma) genes has been identified in the amphibian, Xenopus tropicalis. Like that in other vertebrates such as fish, birds, and mammals, the Xenopus IL-22, IL-26, and IFN-gamma are clustered in the same chromosome and the adjacent genes are conserved. The genomic structures of the Xenopus IL-22, IL-26, and IFN-gamma gene were identical to that of their mammalian counterparts. The Xenopus IL-22 and IL-26 genes contained five exons and four introns while the Xenopus IFN-gamma gene consisted of four exons and three introns. The Xenopus IL-22, IL-26, and IFN-gamma share 14.1-41.6%, 14.6-31.2%, and 23.7-36.5% identity to their counterparts in other species, respectively. Reverse-transcription polymerase chain reaction (PCR) and real-time quantitative PCR analyses revealed that the expression of IL-22, IL-26, and IFN-gamma genes was significantly upregulated after simulation with bacterial polyliposaccharide and/or synthetic double-stranded poly(I:C), suggesting these cytokines like those in other vertebrates play an important role in regulating immune response in Xenopus.; Using bioinformatics approach, the genome locus containing interleukin (IL)-22, IL-26, and interferon gamma (IFN-gamma) genes has been identified in the amphibian, Xenopus tropicalis. Like that in other vertebrates such as fish, birds, and mammals, the Xenopus IL-22, IL-26, and IFN-gamma are clustered in the same chromosome and the adjacent genes are conserved. The genomic structures of the Xenopus IL-22, IL-26, and IFN-gamma gene were identical to that of their mammalian counterparts. The Xenopus IL-22 and IL-26 genes contained five exons and four introns while the Xenopus IFN-gamma gene consisted of four exons and three introns. The Xenopus IL-22, IL-26, and IFN-gamma share 14.1-41.6%, 14.6-31.2%, and 23.7-36.5% identity to their counterparts in other species, respectively. Reverse-transcription polymerase chain reaction (PCR) and real-time quantitative PCR analyses revealed that the expression of IL-22, IL-26, and IFN-gamma genes was significantly upregulated after simulation with bacterial polyliposaccharide and/or synthetic double-stranded poly(I:C), suggesting these cytokines like those in other vertebrates play an important role in regulating immune response in Xenopus.
SubtypeArticle
KeywordInterleukin 22 Interleukin 26 Interferon Gamma Xenopus Tropicalis
Department[Qi, Z. T.; Nie, P.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Qi, Z. T.; Nie, P.] Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Qi, Z. T.] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Subject AreaGenetics & Heredity ; Immunology
DOI10.1007/s00251-008-0326-y
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity ; Immunology
WOS SubjectGenetics & Heredity ; Immunology
WOS IDWOS:000260183000007
WOS KeywordINTERLEUKIN (IL)-22 ; T-CELLS ; TRANSCRIPTION FACTOR ; REGULATORY ELEMENTS ; SEQUENCE ALIGNMENT ; IFN-GAMMA ; IDENTIFICATION ; HOMOLOG ; IL-10 ; RECEPTOR
Funding OrganizationNational Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7978
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Qi, Z. T.,Nie, P.,Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China. Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis[J]. IMMUNOGENETICS,2008,60(11):699-710.
APA Qi, Z. T.,Nie, P.,&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.(2008).Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis.IMMUNOGENETICS,60(11),699-710.
MLA Qi, Z. T.,et al."Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis".IMMUNOGENETICS 60.11(2008):699-710.
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