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The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)
Hao, Le; Xie, Ping; Fu, Juan; Li, Guangyu; Xiong, Qian; Li, Huiying; Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
2008-10-20
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume90Issue:1Pages:1-7
AbstractThe glutathione S-transferases play important roles in the detoxification of microcystin. Core-sequences of three classes of GST (mu, kappa and rho) were cloned from goldfish (Carassius auratus L) i.p. injected with cyanobacterial crude extract at two doses (50 and 200 mu g MC-LReq kg(-1) BW). The relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GST mu was inhibited in intestine at both doses and the transcription of GST kappa was inhibited from 12 to 48 h in kidney at both doses. The decreased transcription of GST rho was detected in all three organs at the high dose. It is suggested that transcription inhibition of GST rho might be significant in MCs toxicity at higher toxin concentration in omnivorous freshwater fish. Alteration in transcription of GSTs stimulated by MCs implicates an increased health risk to fish. (C) 2008 Published by Elsevier B.V.; The glutathione S-transferases play important roles in the detoxification of microcystin. Core-sequences of three classes of GST (mu, kappa and rho) were cloned from goldfish (Carassius auratus L) i.p. injected with cyanobacterial crude extract at two doses (50 and 200 mu g MC-LReq kg(-1) BW). The relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GST mu was inhibited in intestine at both doses and the transcription of GST kappa was inhibited from 12 to 48 h in kidney at both doses. The decreased transcription of GST rho was detected in all three organs at the high dose. It is suggested that transcription inhibition of GST rho might be significant in MCs toxicity at higher toxin concentration in omnivorous freshwater fish. Alteration in transcription of GSTs stimulated by MCs implicates an increased health risk to fish. (C) 2008 Published by Elsevier B.V.
SubtypeArticle
KeywordMicrocystin Glutathione S-transferases Real-time Pcr Goldfish Carassius Auratus l
Department[Hao, Le; Xie, Ping; Fu, Juan; Li, Guangyu; Xiong, Qian; Li, Huiying] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaMarine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2008.07.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000260655000001
WOS KeywordGLUTATHIONE-S-TRANSFERASE ; TROUT ONCORHYNCHUS-MYKISS ; PHYTOPLANKTIVOROUS SILVER CARP ; PLAICE PLEURONECTES-PLATESSA ; HEPATOTOXIN MICROCYSTIN-LR ; HARMFUL ALGAL BLOOMS ; FRESH-WATER FISH ; RED-SEA BREAM ; DANIO-RERIO ; ALPHA-CLASS
Funding OrganizationNational Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101]
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7972
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Hao, Le,Xie, Ping,Fu, Juan,et al. The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)[J]. AQUATIC TOXICOLOGY,2008,90(1):1-7.
APA Hao, Le.,Xie, Ping.,Fu, Juan.,Li, Guangyu.,Xiong, Qian.,...&Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China.(2008).The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus).AQUATIC TOXICOLOGY,90(1),1-7.
MLA Hao, Le,et al."The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)".AQUATIC TOXICOLOGY 90.1(2008):1-7.
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