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Quantitative Determination of Microcystins in Rat Plasma by LC-ESI Tandem MS
Dai, Ming; Xie, Ping; Chen, Jun; Liang, Gaodao; Liu, Ying; Qiu, Tong; Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
2008-11-01
Source PublicationCHROMATOGRAPHIA
ISSN0009-5893
Volume68Issue:9-10Pages:811-815
AbstractA rapid and sensitive method was developed and validated for the determination of MCYST (microcystin)-RR, -LR, and [Dha(7)] MCYST-LR in rat plasma by liquid chromatography-tandem mass spectrometry. The analytes were extracted from rat plasma by protein precipitation, followed by solid-phase extraction. Liquid chromatography with electrospray ionization mass spectrometry, operating in selected reaction monitoring (SRM) mode, was used to quantify MCYST-RR, -LR, and [Dha(7)] MCYST-LR in rat plasma. The recoveries for each analyte in rat plasma ranged from 70.8 to 88.7%. The calibration curve was linear within the range from 0.005 to 1.25 mu g mL(-1). The limit of detection were 1.4, 1.0, 0.6 ng mL(-1) for MCYST-RR, -LR, and [Dha(7)] MCYST-LR. The overall precision was determined on three different days. The values for within- and between-day precision in rat plasma were within 15%. This method was applied to the identification and quantification of microcystins in rat plasma with acute exposure of microcystins via intravenous injection.; A rapid and sensitive method was developed and validated for the determination of MCYST (microcystin)-RR, -LR, and [Dha(7)] MCYST-LR in rat plasma by liquid chromatography-tandem mass spectrometry. The analytes were extracted from rat plasma by protein precipitation, followed by solid-phase extraction. Liquid chromatography with electrospray ionization mass spectrometry, operating in selected reaction monitoring (SRM) mode, was used to quantify MCYST-RR, -LR, and [Dha(7)] MCYST-LR in rat plasma. The recoveries for each analyte in rat plasma ranged from 70.8 to 88.7%. The calibration curve was linear within the range from 0.005 to 1.25 mu g mL(-1). The limit of detection were 1.4, 1.0, 0.6 ng mL(-1) for MCYST-RR, -LR, and [Dha(7)] MCYST-LR. The overall precision was determined on three different days. The values for within- and between-day precision in rat plasma were within 15%. This method was applied to the identification and quantification of microcystins in rat plasma with acute exposure of microcystins via intravenous injection.
SubtypeArticle
KeywordColumn Liquid Chromatography-mass Spectrometry Quantitative Determination MicrocystIns In Rat Plasma
Department[Dai, Ming; Xie, Ping; Chen, Jun; Liang, Gaodao; Liu, Ying; Qiu, Tong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
DOI10.1365/s10337-008-0777-1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationNational Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000260524400018
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; PHOSPHATASE INHIBITION ASSAY ; LINKED-IMMUNOSORBENT-ASSAY ; MASS-SPECTROMETRY ; WATER SAMPLES ; CYANOBACTERIAL HEPATOTOXINS ; CAPILLARY-ELECTROPHORESIS ; PROTEIN PHOSPHATASE-1 ; HUMAN FATALITIES ; TOXINS
Funding OrganizationNational Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077]
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7970
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Dai, Ming,Xie, Ping,Chen, Jun,et al. Quantitative Determination of Microcystins in Rat Plasma by LC-ESI Tandem MS[J]. CHROMATOGRAPHIA,2008,68(9-10):811-815.
APA Dai, Ming.,Xie, Ping.,Chen, Jun.,Liang, Gaodao.,Liu, Ying.,...&Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.(2008).Quantitative Determination of Microcystins in Rat Plasma by LC-ESI Tandem MS.CHROMATOGRAPHIA,68(9-10),811-815.
MLA Dai, Ming,et al."Quantitative Determination of Microcystins in Rat Plasma by LC-ESI Tandem MS".CHROMATOGRAPHIA 68.9-10(2008):811-815.
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