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Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper
Wang, Y.; Zhou, L.; Li, Z.; Gui, J. F.; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan Ctr Dev Biol,Grad Sch, Wuhan 430072, Peoples R China
2008-12-01
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
Volume34Issue:4Pages:339-348
AbstractEndogenous yolk nutrients are crucial for embryo and larval development in fish, but developmental behavior of the genes that control yolk utilization remains unknown. Apolipoproteins have been shown to play important roles in lipid transport and uptake through the circulation system. In this study, EcApoC-I, the first cloned ApoC-I in teleosts, has been screened from pituitary cDNA library of female orange-spotted grouper (Epinephelus coioides), and the deduced amino acid sequence shows 43.5% identity to one zebrafish (Danio rerio) hypothetical protein similar to ApoC-I, and 21.2%, 21.7%, 22.5%, 20%, and 22.5% identities to Apo C-I of human (Homo sapiens), house mouse (Mus musculus), common tree shrew (Tupaia glis), dog (Canis lupus familiaris) and hamadryas baboon (Papio hamadryas), respectively. Although the sequence identity is low, amphipathic alpha-helices with the potential to bind to lipid were predicted to exist in the EcApoC-I. RT-PCR analysis revealed that it was first transcribed in gastrula embryos and maintained a relatively stable expression level during the following embryogenesis. During embryonic and early larval development, a very high level of EcApoC-I expression was in the yolk syncytial layer, indicating that it plays a significant role in yolk degradation and transfers nutrition to the embryo and early larva. By the day 7 after hatching, EcApoC-I transcripts were observed in brain. In adult, EcApoC-I mRNA was detected abundantly in brain and gonad. In transitional gonads, the EcApoC-I expression is restricted to the germ cells. The data suggested that EcApoC-I might play an important role in brain and gonad morphogenesis and growth.; Endogenous yolk nutrients are crucial for embryo and larval development in fish, but developmental behavior of the genes that control yolk utilization remains unknown. Apolipoproteins have been shown to play important roles in lipid transport and uptake through the circulation system. In this study, EcApoC-I, the first cloned ApoC-I in teleosts, has been screened from pituitary cDNA library of female orange-spotted grouper (Epinephelus coioides), and the deduced amino acid sequence shows 43.5% identity to one zebrafish (Danio rerio) hypothetical protein similar to ApoC-I, and 21.2%, 21.7%, 22.5%, 20%, and 22.5% identities to Apo C-I of human (Homo sapiens), house mouse (Mus musculus), common tree shrew (Tupaia glis), dog (Canis lupus familiaris) and hamadryas baboon (Papio hamadryas), respectively. Although the sequence identity is low, amphipathic alpha-helices with the potential to bind to lipid were predicted to exist in the EcApoC-I. RT-PCR analysis revealed that it was first transcribed in gastrula embryos and maintained a relatively stable expression level during the following embryogenesis. During embryonic and early larval development, a very high level of EcApoC-I expression was in the yolk syncytial layer, indicating that it plays a significant role in yolk degradation and transfers nutrition to the embryo and early larva. By the day 7 after hatching, EcApoC-I transcripts were observed in brain. In adult, EcApoC-I mRNA was detected abundantly in brain and gonad. In transitional gonads, the EcApoC-I expression is restricted to the germ cells. The data suggested that EcApoC-I might play an important role in brain and gonad morphogenesis and growth.
SubtypeArticle
KeywordApolipoproteins Apoc-i Grouper Yolk Syncytial Layer
Department[Wang, Y.; Zhou, L.; Li, Z.; Gui, J. F.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan Ctr Dev Biol,Grad Sch, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
DOI10.1007/s10695-007-9193-y
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS SubjectBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS IDWOS:000260512800006
WOS KeywordYOLK SYNCYTIAL LAYER ; LOW-DENSITY LIPOPROTEINS ; APOLIPOPROTEIN MULTIGENE FAMILY ; TURBOT SCOPHTHALMUS-MAXIMUS ; FARIO-TRUTTA L ; EPINEPHELUS-COIOIDES ; C-I ; PLASMA APOLIPOPROTEINS ; SEX REVERSAL ; DIFFERENTIAL EXPRESSION
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7968
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan Ctr Dev Biol,Grad Sch, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan Ctr Dev Biol,Grad Sch, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Y.,Zhou, L.,Li, Z.,et al. Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2008,34(4):339-348.
APA Wang, Y.,Zhou, L.,Li, Z.,Gui, J. F.,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan Ctr Dev Biol,Grad Sch, Wuhan 430072, Peoples R China.(2008).Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper.FISH PHYSIOLOGY AND BIOCHEMISTRY,34(4),339-348.
MLA Wang, Y.,et al."Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper".FISH PHYSIOLOGY AND BIOCHEMISTRY 34.4(2008):339-348.
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