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Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae)
Hu, Zhengyu1,2; Li, Yantao1,3; Sommerfeld, Milton1; Chen, Feng3; Hu, Qiang1; Hu, Q, Arizona State Univ, Dept Appl Biol Sci, Polytech Campus,7001 E Williams Field Rd, Mesa, AZ 85212 USA
2008
Source PublicationEUROPEAN JOURNAL OF PHYCOLOGY
ISSN0967-0262
Volume43Issue:4Pages:365-376
AbstractMany unicellular green algae can become yellow or red in various natural habitats due to mass accumulation of a secondary carotenoid, such as lutein, or astaxanthin. The accumulation of secondary carotenoids is generally thought to be a survival strategy of the algae under photo-oxidative stress or other adverse environmental conditions. The physiological role of the carotenoids in stress response is less well understood at the subcellular or molecular level. In this study, a stable astaxanthin overproduction mutant (MT 2877) was isolated by chemical mutagenesis of a wild type (WT) of the green microalga Haematococcus pluvialis Flotow NIES-144. MT 2877 was identical to the WT with respect to morphology, pigment composition, and growth kinetics during the early vegetative stage of the life cycle. However, it had the ability to synthesize and accumulate about twice the astaxanthin content of the WT under high light, or under high light in the presence of excess amounts of ferrous sulphate and sodium acetate. Under stress, the mutant exhibited higher photosynthetic activities than the WT, based on considerably higher chlorophyll fluorescence induction, chlorophyll autofluorescence intensities, and oxygen evolution rates. Cell mortality caused by stress was reduced by half in the mutant culture compared with the WT. Enhanced protection of the mutant against stress is attributed to its accelerated carotenogenesis and accumulation of astaxanthin. Our results suggest that MT 2877, or other astaxanthin overproduction Haematococcus mutants, may offer dual benefits, as compared with the wild type, by increasing cellular astaxanthin content while reducing cell mortality during stress-induced carotenogenesis.; Many unicellular green algae can become yellow or red in various natural habitats due to mass accumulation of a secondary carotenoid, such as lutein, or astaxanthin. The accumulation of secondary carotenoids is generally thought to be a survival strategy of the algae under photo-oxidative stress or other adverse environmental conditions. The physiological role of the carotenoids in stress response is less well understood at the subcellular or molecular level. In this study, a stable astaxanthin overproduction mutant (MT 2877) was isolated by chemical mutagenesis of a wild type (WT) of the green microalga Haematococcus pluvialis Flotow NIES-144. MT 2877 was identical to the WT with respect to morphology, pigment composition, and growth kinetics during the early vegetative stage of the life cycle. However, it had the ability to synthesize and accumulate about twice the astaxanthin content of the WT under high light, or under high light in the presence of excess amounts of ferrous sulphate and sodium acetate. Under stress, the mutant exhibited higher photosynthetic activities than the WT, based on considerably higher chlorophyll fluorescence induction, chlorophyll autofluorescence intensities, and oxygen evolution rates. Cell mortality caused by stress was reduced by half in the mutant culture compared with the WT. Enhanced protection of the mutant against stress is attributed to its accelerated carotenogenesis and accumulation of astaxanthin. Our results suggest that MT 2877, or other astaxanthin overproduction Haematococcus mutants, may offer dual benefits, as compared with the wild type, by increasing cellular astaxanthin content while reducing cell mortality during stress-induced carotenogenesis.
SubtypeArticle
KeywordAstaxanthin Carotenogenesis Flow Cytometry Haematococcus Pluvialis Oxidative Stress Pigment Mutant
Department[Hu, Zhengyu; Li, Yantao; Sommerfeld, Milton; Hu, Qiang] Arizona State Univ, Dept Appl Biol Sci, Mesa, AZ 85212 USA; [Hu, Zhengyu] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430080, Hubei, Peoples R China; [Li, Yantao; Chen, Feng] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China
Subject AreaPlant Sciences ; Marine & Freshwater Biology
DOI10.1080/09670260802227736
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationScience Foundation Arizona's Small Business Catalytic Program ; Science Foundation Arizona's Small Business Catalytic Program ; Science Foundation Arizona's Small Business Catalytic Program ; Science Foundation Arizona's Small Business Catalytic Program
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000261194700004
WOS KeywordGIROD ROSTAFINSKI VOLVOCALES ; GREEN-ALGA ; SECONDARY CAROTENOIDS ; PLUVIALIS CHLOROPHYCEAE ; FUNCTIONAL-ASPECTS ; CHLAMYDOMONAS-REINHARDI ; ANTIOXIDANT ROLE ; HIGH IRRADIANCE ; ACCUMULATION ; LACUSTRIS
Funding OrganizationScience Foundation Arizona's Small Business Catalytic Program ; Science Foundation Arizona's Small Business Catalytic Program ; Science Foundation Arizona's Small Business Catalytic Program ; Science Foundation Arizona's Small Business Catalytic Program
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7958
Collection期刊论文
Corresponding AuthorHu, Q, Arizona State Univ, Dept Appl Biol Sci, Polytech Campus,7001 E Williams Field Rd, Mesa, AZ 85212 USA
Affiliation1.Arizona State Univ, Dept Appl Biol Sci, Mesa, AZ 85212 USA
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430080, Hubei, Peoples R China
3.Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Hu, Zhengyu,Li, Yantao,Sommerfeld, Milton,et al. Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae)[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2008,43(4):365-376.
APA Hu, Zhengyu,Li, Yantao,Sommerfeld, Milton,Chen, Feng,Hu, Qiang,&Hu, Q, Arizona State Univ, Dept Appl Biol Sci, Polytech Campus,7001 E Williams Field Rd, Mesa, AZ 85212 USA.(2008).Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae).EUROPEAN JOURNAL OF PHYCOLOGY,43(4),365-376.
MLA Hu, Zhengyu,et al."Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae)".EUROPEAN JOURNAL OF PHYCOLOGY 43.4(2008):365-376.
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