| Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus | |
| Zhou, G. Z.; Gui, L.; Li, Z. Q.; Yuan, X. P.; Zhang, Q. Y.; Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2008-12-01 | |
| Source Publication | JOURNAL OF FISH BIOLOGY
 |
| ISSN | 0022-1112 |
| Volume | 73Issue:8Pages:2058-2067 |
| Abstract | Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a 'living fossil' on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail-fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco's modified Eagle's medium at 25 degrees C. Karyotypic analysis revealed a normal diploid karyotype with 2n = 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron-microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV-infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV-infected CSTF cells' DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.; Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a 'living fossil' on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail-fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco's modified Eagle's medium at 25 degrees C. Karyotypic analysis revealed a normal diploid karyotype with 2n = 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron-microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV-infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV-infected CSTF cells' DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies. |
| Subtype | Article |
| Keyword | Chromosome Cytopathic Effect Electron Microscope Freshwater Fish Immunofluorescence Karyotype |
| Department | [Zhou, G. Z.; Gui, L.; Li, Z. Q.; Yuan, X. P.; Zhang, Q. Y.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Subject Area | Fisheries ; Marine & Freshwater Biology |
| DOI | 10.1111/j.1095-8649.2008.02076.x |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000261252300014 |
| WOS Keyword | RANA-GRYLIO VIRUS ; WHITE STURGEON ; INFECTION |
| Funding Organization | National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7954 |
| Collection | 期刊论文 |
| Corresponding Author | Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zhou, G. Z.,Gui, L.,Li, Z. Q.,et al. Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus[J]. JOURNAL OF FISH BIOLOGY,2008,73(8):2058-2067. |
| APA | Zhou, G. Z.,Gui, L.,Li, Z. Q.,Yuan, X. P.,Zhang, Q. Y.,&Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus.JOURNAL OF FISH BIOLOGY,73(8),2058-2067. |
| MLA | Zhou, G. Z.,et al."Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus".JOURNAL OF FISH BIOLOGY 73.8(2008):2058-2067. |
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