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Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus
Zhou, G. Z.; Gui, L.; Li, Z. Q.; Yuan, X. P.; Zhang, Q. Y.; Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-12-01
Source PublicationJOURNAL OF FISH BIOLOGY
ISSN0022-1112
Volume73Issue:8Pages:2058-2067
AbstractChinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a 'living fossil' on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail-fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco's modified Eagle's medium at 25 degrees C. Karyotypic analysis revealed a normal diploid karyotype with 2n = 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron-microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV-infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV-infected CSTF cells' DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.; Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a 'living fossil' on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail-fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco's modified Eagle's medium at 25 degrees C. Karyotypic analysis revealed a normal diploid karyotype with 2n = 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron-microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV-infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV-infected CSTF cells' DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.
SubtypeArticle
KeywordChromosome Cytopathic Effect Electron Microscope Freshwater Fish Immunofluorescence Karyotype
Department[Zhou, G. Z.; Gui, L.; Li, Z. Q.; Yuan, X. P.; Zhang, Q. Y.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1111/j.1095-8649.2008.02076.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000261252300014
WOS KeywordRANA-GRYLIO VIRUS ; WHITE STURGEON ; INFECTION
Funding OrganizationNational Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] ; National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05]
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7954
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, G. Z.,Gui, L.,Li, Z. Q.,et al. Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus[J]. JOURNAL OF FISH BIOLOGY,2008,73(8):2058-2067.
APA Zhou, G. Z.,Gui, L.,Li, Z. Q.,Yuan, X. P.,Zhang, Q. Y.,&Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus.JOURNAL OF FISH BIOLOGY,73(8),2058-2067.
MLA Zhou, G. Z.,et al."Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus".JOURNAL OF FISH BIOLOGY 73.8(2008):2058-2067.
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