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Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide
Zhang, Fu-Tie; Zhang, Yi-Bing; Chen, Yu-Dong; Zhu, Rong; Dong, Cai-Wen; Li, Yang-Yang; Zhang, Qi-Ya; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume25Issue:5Pages:542-549
AbstractCathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions. In this study, Paralichthys olivaceus cathepsin B (PoCatB) cDNA was isolated from flounder embryonic cells (FEC) treated with UV-inactivated grass carp hemorrhage virus (GCHV) and subsequently identified as a vitally induced gene. The full length cDNA of PoCatB is 1801 bp encoding 330-amino acids. The deduced protein has high homology to all known cathepsin B proteins, containing an N-terminal signal peptide, cysteine protease active sites, the occluding loop segment and a glycosylation site, all of which are conserved in the cathepsin B family. PoCatB transcription of FEC cells could be induced by turbot (Scophthalmus maximus) rhabdovirus (SMRV), UV-inactivated SMRV, UV-inactivated GCHV, poly I:C or lipopolysaccharide (LPS), and SMRV or poly I:C was revealed to be most effective among the five inducers. In normal flounder, PoCatB mRNA was detectable in all examined tissues. Moreover, SMRV infection could result in significant upregulation of PoCatB mRNA, predominantly in spleen, head kidney, posterior kidney, intestine, gill and muscle with 18.2,10.9, 24.7,12, 31.5 and 18 fold increases at 72 h post-infection respectively. These results provided the first evidence for the transcriptional induction of cathepsin B in fish by virus and LPS, indicating existence of a novel function in viral defense. (C) 2008 Elsevier Ltd. All rights reserved.; Cathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions. In this study, Paralichthys olivaceus cathepsin B (PoCatB) cDNA was isolated from flounder embryonic cells (FEC) treated with UV-inactivated grass carp hemorrhage virus (GCHV) and subsequently identified as a vitally induced gene. The full length cDNA of PoCatB is 1801 bp encoding 330-amino acids. The deduced protein has high homology to all known cathepsin B proteins, containing an N-terminal signal peptide, cysteine protease active sites, the occluding loop segment and a glycosylation site, all of which are conserved in the cathepsin B family. PoCatB transcription of FEC cells could be induced by turbot (Scophthalmus maximus) rhabdovirus (SMRV), UV-inactivated SMRV, UV-inactivated GCHV, poly I:C or lipopolysaccharide (LPS), and SMRV or poly I:C was revealed to be most effective among the five inducers. In normal flounder, PoCatB mRNA was detectable in all examined tissues. Moreover, SMRV infection could result in significant upregulation of PoCatB mRNA, predominantly in spleen, head kidney, posterior kidney, intestine, gill and muscle with 18.2,10.9, 24.7,12, 31.5 and 18 fold increases at 72 h post-infection respectively. These results provided the first evidence for the transcriptional induction of cathepsin B in fish by virus and LPS, indicating existence of a novel function in viral defense. (C) 2008 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordFlounder (Paralichthys Olivaceus) Cathepsin b Expressional Induction Lipopolysaccharide (Lps) Virus Infection
Department[Zhang, Fu-Tie; Zhang, Yi-Bing; Chen, Yu-Dong; Zhu, Rong; Dong, Cai-Wen; Li, Yang-Yang; Zhang, Qi-Ya; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2008.07.018
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization973 National Basic Research Program of China [2004CB117403]; National Natural Science Foundation of China [30471333, 30671617]; Innovation group project of Hubei Province [2004ABC005] ; 973 National Basic Research Program of China [2004CB117403]; National Natural Science Foundation of China [30471333, 30671617]; Innovation group project of Hubei Province [2004ABC005] ; 973 National Basic Research Program of China [2004CB117403]; National Natural Science Foundation of China [30471333, 30671617]; Innovation group project of Hubei Province [2004ABC005] ; 973 National Basic Research Program of China [2004CB117403]; National Natural Science Foundation of China [30471333, 30671617]; Innovation group project of Hubei Province [2004ABC005]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000261564400011
WOS KeywordPEPTIDE PARASIN-I ; MOLECULAR-CLONING ; JAPANESE FLOUNDER ; CELL-LINE ; ENDOSOMAL PROTEOLYSIS ; CYSTEINE PROTEINASES ; PROCESSING ENZYMES ; OOCYTE MATURATION ; THP-1 CELLS ; SKIN MUCOSA
Funding Organization973 National Basic Research Program of China [2004CB117403]; National Natural Science Foundation of China [30471333, 30671617]; Innovation group project of Hubei Province [2004ABC005] ; 973 National Basic Research Program of China [2004CB117403]; National Natural Science Foundation of China [30471333, 30671617]; Innovation group project of Hubei Province [2004ABC005] ; 973 National Basic Research Program of China [2004CB117403]; National Natural Science Foundation of China [30471333, 30671617]; Innovation group project of Hubei Province [2004ABC005] ; 973 National Basic Research Program of China [2004CB117403]; National Natural Science Foundation of China [30471333, 30671617]; Innovation group project of Hubei Province [2004ABC005]
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Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7946
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Fu-Tie,Zhang, Yi-Bing,Chen, Yu-Dong,et al. Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide[J]. FISH & SHELLFISH IMMUNOLOGY,2008,25(5):542-549.
APA Zhang, Fu-Tie.,Zhang, Yi-Bing.,Chen, Yu-Dong.,Zhu, Rong.,Dong, Cai-Wen.,...&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide.FISH & SHELLFISH IMMUNOLOGY,25(5),542-549.
MLA Zhang, Fu-Tie,et al."Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide".FISH & SHELLFISH IMMUNOLOGY 25.5(2008):542-549.
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