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The role of cell-surface-bound phosphatases in species competition within natural phytoplankton assemblage: an in situ experiment
Strojsova, Alena1,2; Nedoma, Jiri1; Strojsova, Martina1,2; Cao, Xiuyun3; Vrba, Jaroslav1,2; Vrba, J, Univ S Bohemia, Fac Sci, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic
2008
Source PublicationJOURNAL OF LIMNOLOGY
ISSN1129-5767
Volume67Issue:2Pages:128-138
AbstractDespite it is widely acknowledged that the ability to hydrolyze dissolved organic matter using extracellular phosphatases is diverse in fresh water phytoplankton, the competition within single species related to presence and quantity of cell-surface-bound phosphatases has not been examined in natural conditions yet. Here, we studied phytoplankton species competition in a freshwater reservoir during an in situ experiment. A natural plankton community, with the exclusion of large zooplankton, was enclosed in permeable dialysis bags inside two large containers of different bioavailable phosphate concentrations. Phytoplankton species biomass and the abundance of bacteria were determined in purpose to compare the development of enclosed microbial communities. Total and cell-surface-bound phosphatase activities in the phytoplankton were investigated using the Fluorescently Labelled Enzyme Activity (FLEA) technique that allows for direct microscopic detection of phosphatase-positive cells and, with image cytometry, enables quantification of phosphatase hydrolytic capacity. Production of extracellular phosphatases was not completely inhibited or stopped in the phosphate-enriched environment, phytoplankton cells only showed the activity less often. Under the phosphate-nonenriched conditions, the production of phosphatases was enhanced, but active species did not proliferate amongst phytoplankton assemblage. Further, specific growth rates of the phosphatase-positive species in the non-enriched environment were lower than the same phosphatase-positive species in phosphate-enriched environment. Interestingly, the phosphatase-positive cells of Ankyra ancora increased their size in both treatments equally, although the population in phosphate-enriched environment grew much faster and the cell-specific phosphatase activity was lower. We hypothesize that brand new daughter cells had sufficient phosphorus reserves and therefore did not employ extracellular phosphatases until they matured and needed extra bioavailable phosphorus to support their metabolism before cell division. Based on presented in situ experiment, we propose that the ability to hydrolyze organic polymers and particles with cell-surface-hound phosphatases is advantageous for longer persistence of given population in a phosphate-scarce environment; although phosphatase-positive species cannot dominate the reservoir phytoplankton solely because of specific phosphorus-scavenging strategy.; Despite it is widely acknowledged that the ability to hydrolyze dissolved organic matter using extracellular phosphatases is diverse in fresh water phytoplankton, the competition within single species related to presence and quantity of cell-surface-bound phosphatases has not been examined in natural conditions yet. Here, we studied phytoplankton species competition in a freshwater reservoir during an in situ experiment. A natural plankton community, with the exclusion of large zooplankton, was enclosed in permeable dialysis bags inside two large containers of different bioavailable phosphate concentrations. Phytoplankton species biomass and the abundance of bacteria were determined in purpose to compare the development of enclosed microbial communities. Total and cell-surface-bound phosphatase activities in the phytoplankton were investigated using the Fluorescently Labelled Enzyme Activity (FLEA) technique that allows for direct microscopic detection of phosphatase-positive cells and, with image cytometry, enables quantification of phosphatase hydrolytic capacity. Production of extracellular phosphatases was not completely inhibited or stopped in the phosphate-enriched environment, phytoplankton cells only showed the activity less often. Under the phosphate-nonenriched conditions, the production of phosphatases was enhanced, but active species did not proliferate amongst phytoplankton assemblage. Further, specific growth rates of the phosphatase-positive species in the non-enriched environment were lower than the same phosphatase-positive species in phosphate-enriched environment. Interestingly, the phosphatase-positive cells of Ankyra ancora increased their size in both treatments equally, although the population in phosphate-enriched environment grew much faster and the cell-specific phosphatase activity was lower. We hypothesize that brand new daughter cells had sufficient phosphorus reserves and therefore did not employ extracellular phosphatases until they matured and needed extra bioavailable phosphorus to support their metabolism before cell division. Based on presented in situ experiment, we propose that the ability to hydrolyze organic polymers and particles with cell-surface-hound phosphatases is advantageous for longer persistence of given population in a phosphate-scarce environment; although phosphatase-positive species cannot dominate the reservoir phytoplankton solely because of specific phosphorus-scavenging strategy.
SubtypeArticle
KeywordCompetition Ectoenzyme Elf97 Phosphate Image Cytometry Species-specific Activity
Department[Strojsova, Alena; Nedoma, Jiri; Strojsova, Martina; Vrba, Jaroslav] Univ S Bohemia, Fac Sci, CZ-37005 Ceske Budejovice, Czech Republic; [Strojsova, Alena; Strojsova, Martina; Vrba, Jaroslav] Biol Ctr AS CR, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic; [Cao, Xiuyun] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaLimnology
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300] ; ASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300] ; ASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300] ; ASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectLimnology
WOS IDWOS:000261475800007
WOS KeywordENZYME-LABELED FLUORESCENCE ; FRESH-WATER PHYTOPLANKTON ; ALKALINE-PHOSPHATASE ; EXTRACELLULAR PHOSPHATASES ; MARINE-PHYTOPLANKTON ; NUTRIENT LIMITATION ; EUTROPHIC RESERVOIR ; DIGESTIVE ENZYMES ; FIELD POPULATIONS ; PHOSPHORUS STATUS
Funding OrganizationASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300] ; ASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300] ; ASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300] ; ASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7942
Collection期刊论文
Corresponding AuthorVrba, J, Univ S Bohemia, Fac Sci, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic
Affiliation1.Univ S Bohemia, Fac Sci, CZ-37005 Ceske Budejovice, Czech Republic
2.Biol Ctr AS CR, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Strojsova, Alena,Nedoma, Jiri,Strojsova, Martina,et al. The role of cell-surface-bound phosphatases in species competition within natural phytoplankton assemblage: an in situ experiment[J]. JOURNAL OF LIMNOLOGY,2008,67(2):128-138.
APA Strojsova, Alena,Nedoma, Jiri,Strojsova, Martina,Cao, Xiuyun,Vrba, Jaroslav,&Vrba, J, Univ S Bohemia, Fac Sci, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic.(2008).The role of cell-surface-bound phosphatases in species competition within natural phytoplankton assemblage: an in situ experiment.JOURNAL OF LIMNOLOGY,67(2),128-138.
MLA Strojsova, Alena,et al."The role of cell-surface-bound phosphatases in species competition within natural phytoplankton assemblage: an in situ experiment".JOURNAL OF LIMNOLOGY 67.2(2008):128-138.
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