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学科主题: Limnology
题名: The role of cell-surface-bound phosphatases in species competition within natural phytoplankton assemblage: an in situ experiment
作者: Strojsova, Alena1, 2; Nedoma, Jiri1; Strojsova, Martina1, 2; Cao, Xiuyun3; Vrba, Jaroslav1, 2
通讯作者: Vrba, J, Univ S Bohemia, Fac Sci, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic
关键词: competition ; ectoenzyme ; ELF97 phosphate ; image cytometry ; species-specific activity
刊名: JOURNAL OF LIMNOLOGY
发表日期: 2008
卷: 67, 期:2, 页:128-138
收录类别: SCI
文章类型: Article
部门归属: [Strojsova, Alena; Nedoma, Jiri; Strojsova, Martina; Vrba, Jaroslav] Univ S Bohemia, Fac Sci, CZ-37005 Ceske Budejovice, Czech Republic; [Strojsova, Alena; Strojsova, Martina; Vrba, Jaroslav] Biol Ctr AS CR, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic; [Cao, Xiuyun] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: ASCR [6017202, A600170602]; Czech Ministry of Education, Youth and Sports [ME 617, MSM 6007665801]; National Basic Research Program of China [2002CB412300]
类目[WOS]: Limnology
研究领域[WOS]: Marine & Freshwater Biology
摘要: Despite it is widely acknowledged that the ability to hydrolyze dissolved organic matter using extracellular phosphatases is diverse in fresh water phytoplankton, the competition within single species related to presence and quantity of cell-surface-bound phosphatases has not been examined in natural conditions yet. Here, we studied phytoplankton species competition in a freshwater reservoir during an in situ experiment. A natural plankton community, with the exclusion of large zooplankton, was enclosed in permeable dialysis bags inside two large containers of different bioavailable phosphate concentrations. Phytoplankton species biomass and the abundance of bacteria were determined in purpose to compare the development of enclosed microbial communities. Total and cell-surface-bound phosphatase activities in the phytoplankton were investigated using the Fluorescently Labelled Enzyme Activity (FLEA) technique that allows for direct microscopic detection of phosphatase-positive cells and, with image cytometry, enables quantification of phosphatase hydrolytic capacity. Production of extracellular phosphatases was not completely inhibited or stopped in the phosphate-enriched environment, phytoplankton cells only showed the activity less often. Under the phosphate-nonenriched conditions, the production of phosphatases was enhanced, but active species did not proliferate amongst phytoplankton assemblage. Further, specific growth rates of the phosphatase-positive species in the non-enriched environment were lower than the same phosphatase-positive species in phosphate-enriched environment. Interestingly, the phosphatase-positive cells of Ankyra ancora increased their size in both treatments equally, although the population in phosphate-enriched environment grew much faster and the cell-specific phosphatase activity was lower. We hypothesize that brand new daughter cells had sufficient phosphorus reserves and therefore did not employ extracellular phosphatases until they matured and needed extra bioavailable phosphorus to support their metabolism before cell division. Based on presented in situ experiment, we propose that the ability to hydrolyze organic polymers and particles with cell-surface-hound phosphatases is advantageous for longer persistence of given population in a phosphate-scarce environment; although phosphatase-positive species cannot dominate the reservoir phytoplankton solely because of specific phosphorus-scavenging strategy.
英文摘要: Despite it is widely acknowledged that the ability to hydrolyze dissolved organic matter using extracellular phosphatases is diverse in fresh water phytoplankton, the competition within single species related to presence and quantity of cell-surface-bound phosphatases has not been examined in natural conditions yet. Here, we studied phytoplankton species competition in a freshwater reservoir during an in situ experiment. A natural plankton community, with the exclusion of large zooplankton, was enclosed in permeable dialysis bags inside two large containers of different bioavailable phosphate concentrations. Phytoplankton species biomass and the abundance of bacteria were determined in purpose to compare the development of enclosed microbial communities. Total and cell-surface-bound phosphatase activities in the phytoplankton were investigated using the Fluorescently Labelled Enzyme Activity (FLEA) technique that allows for direct microscopic detection of phosphatase-positive cells and, with image cytometry, enables quantification of phosphatase hydrolytic capacity. Production of extracellular phosphatases was not completely inhibited or stopped in the phosphate-enriched environment, phytoplankton cells only showed the activity less often. Under the phosphate-nonenriched conditions, the production of phosphatases was enhanced, but active species did not proliferate amongst phytoplankton assemblage. Further, specific growth rates of the phosphatase-positive species in the non-enriched environment were lower than the same phosphatase-positive species in phosphate-enriched environment. Interestingly, the phosphatase-positive cells of Ankyra ancora increased their size in both treatments equally, although the population in phosphate-enriched environment grew much faster and the cell-specific phosphatase activity was lower. We hypothesize that brand new daughter cells had sufficient phosphorus reserves and therefore did not employ extracellular phosphatases until they matured and needed extra bioavailable phosphorus to support their metabolism before cell division. Based on presented in situ experiment, we propose that the ability to hydrolyze organic polymers and particles with cell-surface-hound phosphatases is advantageous for longer persistence of given population in a phosphate-scarce environment; although phosphatase-positive species cannot dominate the reservoir phytoplankton solely because of specific phosphorus-scavenging strategy.
关键词[WOS]: ENZYME-LABELED FLUORESCENCE ; FRESH-WATER PHYTOPLANKTON ; ALKALINE-PHOSPHATASE ; EXTRACELLULAR PHOSPHATASES ; MARINE-PHYTOPLANKTON ; NUTRIENT LIMITATION ; EUTROPHIC RESERVOIR ; DIGESTIVE ENZYMES ; FIELD POPULATIONS ; PHOSPHORUS STATUS
语种: 英语
WOS记录号: WOS:000261475800007
ISSN号: 1129-5767
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7942
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Univ S Bohemia, Fac Sci, CZ-37005 Ceske Budejovice, Czech Republic
2.Biol Ctr AS CR, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Strojsova, Alena; Nedoma, Jiri; Strojsova, Martina; Cao, Xiuyun; Vrba, Jaroslav.The role of cell-surface-bound phosphatases in species competition within natural phytoplankton assemblage: an in situ experiment,JOURNAL OF LIMNOLOGY,2008,67(2):128-138
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