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Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP
Liu, J.1; Sun, Y. H.1; Wang, Y. W.1,2; Wang, N.1; Pei, D. S.1; Wang, Y. P.1; Hu, W.1; Zhu, Z. Y.1; Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-12-01
Source PublicationTHERIOGENOLOGY
ISSN0093-691X
Volume70Issue:9Pages:1525-1535
AbstractThe crosstalk between naive nucleus and maternal factors deposited in egg cytoplasm before zygotic genome activation is crucial for early development. In this study, we utilized two laboratory fishes, zebrafish (Danio rerio) and Chinese rare minnow and Chinese rare minnow (Gobiocypris rarus), to obtain mutual crossbred embroys and examine the interaction between nucleus and egg cytoplasm from different species. Although these two types of crossbred embryos originated from common nuclei, various developmental capacities were gained due to different origins of the egg cytoplasm. Using cDNA amplified fragment length polymorphism (cDNA-AFLP), We Compared transcript profiles between the mutual crossbred embryos at two developmental stages (50%- and 90%-epiholy). Three thousand cDNA fragments were generated in four cDNA pools with 64 primer combinations. All differently displayed transcript-derived fragments (TDFs) were screened by (lot blot hybridization, and the selected sequences were further analyzed by semi-quantitative RT-PCR and quantitative real-time RT-PCR. Compared with ZR embryos, 12 genes were up-regulated and 12 were down-regulated in RZ embryos. The gene fragments were sequenced and subjected to BLASTN analysis. The sequences encoded various proteins which functioned at various levels of proliferation, growth, and development. One gene (ZR6), dramatically down-regulated in RZ embryos, was chosen for loss-of-function study; the knockdown of ZR6 gave rise to the phenotype resembling that of RZ embryos. (c) 2008 Elsevier Inc. All rights reserved.; The crosstalk between naive nucleus and maternal factors deposited in egg cytoplasm before zygotic genome activation is crucial for early development. In this study, we utilized two laboratory fishes, zebrafish (Danio rerio) and Chinese rare minnow and Chinese rare minnow (Gobiocypris rarus), to obtain mutual crossbred embroys and examine the interaction between nucleus and egg cytoplasm from different species. Although these two types of crossbred embryos originated from common nuclei, various developmental capacities were gained due to different origins of the egg cytoplasm. Using cDNA amplified fragment length polymorphism (cDNA-AFLP), We Compared transcript profiles between the mutual crossbred embryos at two developmental stages (50%- and 90%-epiholy). Three thousand cDNA fragments were generated in four cDNA pools with 64 primer combinations. All differently displayed transcript-derived fragments (TDFs) were screened by (lot blot hybridization, and the selected sequences were further analyzed by semi-quantitative RT-PCR and quantitative real-time RT-PCR. Compared with ZR embryos, 12 genes were up-regulated and 12 were down-regulated in RZ embryos. The gene fragments were sequenced and subjected to BLASTN analysis. The sequences encoded various proteins which functioned at various levels of proliferation, growth, and development. One gene (ZR6), dramatically down-regulated in RZ embryos, was chosen for loss-of-function study; the knockdown of ZR6 gave rise to the phenotype resembling that of RZ embryos. (c) 2008 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordZebrafish Chinese Rare Minnow Crossbred Embryo Cdna-aflp Differential Expression
Department[Liu, J.; Sun, Y. H.; Wang, Y. W.; Wang, N.; Pei, D. S.; Wang, Y. P.; Hu, W.; Zhu, Z. Y.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Wang, Y. W.] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Subject AreaReproductive Biology ; Veterinary Sciences
DOI10.1016/j.theriogenology.2008.07.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationState Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100]
Indexed BySCI
Language英语
WOS Research AreaReproductive Biology ; Veterinary Sciences
WOS SubjectReproductive Biology ; Veterinary Sciences
WOS IDWOS:000261285000016
WOS KeywordCELL NUCLEAR TRANSFER ; GENE-EXPRESSION ; ENUCLEATED EGGS ; XENOMITOCHONDRIAL CYBRIDS ; SACCHAROMYCES-CEREVISIAE ; GENOME ; MITOCHONDRIA ; DEFICIENCY ; STABILITY ; IMPACT
Funding OrganizationState Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7938
Collection期刊论文
Corresponding AuthorSun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu, J.,Sun, Y. H.,Wang, Y. W.,et al. Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP[J]. THERIOGENOLOGY,2008,70(9):1525-1535.
APA Liu, J..,Sun, Y. H..,Wang, Y. W..,Wang, N..,Pei, D. S..,...&Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP.THERIOGENOLOGY,70(9),1525-1535.
MLA Liu, J.,et al."Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP".THERIOGENOLOGY 70.9(2008):1525-1535.
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