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学科主题: Reproductive Biology; Veterinary Sciences
题名: Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP
作者: Liu, J.1; Sun, Y. H.1; Wang, Y. W.1, 2; Wang, N.1; Pei, D. S.1; Wang, Y. P.1; Hu, W.1; Zhu, Z. Y.1
通讯作者: Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Zebrafish ; Chinese rare minnow ; Crossbred embryo ; cDNA-AFLP ; Differential expression
刊名: THERIOGENOLOGY
发表日期: 2008-12-01
DOI: 10.1016/j.theriogenology.2008.07.001
卷: 70, 期:9, 页:1525-1535
收录类别: SCI
文章类型: Article
部门归属: [Liu, J.; Sun, Y. H.; Wang, Y. W.; Wang, N.; Pei, D. S.; Wang, Y. P.; Hu, W.; Zhu, Z. Y.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Wang, Y. W.] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: State Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100]
类目[WOS]: Reproductive Biology ; Veterinary Sciences
研究领域[WOS]: Reproductive Biology ; Veterinary Sciences
摘要: The crosstalk between naive nucleus and maternal factors deposited in egg cytoplasm before zygotic genome activation is crucial for early development. In this study, we utilized two laboratory fishes, zebrafish (Danio rerio) and Chinese rare minnow and Chinese rare minnow (Gobiocypris rarus), to obtain mutual crossbred embroys and examine the interaction between nucleus and egg cytoplasm from different species. Although these two types of crossbred embryos originated from common nuclei, various developmental capacities were gained due to different origins of the egg cytoplasm. Using cDNA amplified fragment length polymorphism (cDNA-AFLP), We Compared transcript profiles between the mutual crossbred embryos at two developmental stages (50%- and 90%-epiholy). Three thousand cDNA fragments were generated in four cDNA pools with 64 primer combinations. All differently displayed transcript-derived fragments (TDFs) were screened by (lot blot hybridization, and the selected sequences were further analyzed by semi-quantitative RT-PCR and quantitative real-time RT-PCR. Compared with ZR embryos, 12 genes were up-regulated and 12 were down-regulated in RZ embryos. The gene fragments were sequenced and subjected to BLASTN analysis. The sequences encoded various proteins which functioned at various levels of proliferation, growth, and development. One gene (ZR6), dramatically down-regulated in RZ embryos, was chosen for loss-of-function study; the knockdown of ZR6 gave rise to the phenotype resembling that of RZ embryos. (c) 2008 Elsevier Inc. All rights reserved.
英文摘要: The crosstalk between naive nucleus and maternal factors deposited in egg cytoplasm before zygotic genome activation is crucial for early development. In this study, we utilized two laboratory fishes, zebrafish (Danio rerio) and Chinese rare minnow and Chinese rare minnow (Gobiocypris rarus), to obtain mutual crossbred embroys and examine the interaction between nucleus and egg cytoplasm from different species. Although these two types of crossbred embryos originated from common nuclei, various developmental capacities were gained due to different origins of the egg cytoplasm. Using cDNA amplified fragment length polymorphism (cDNA-AFLP), We Compared transcript profiles between the mutual crossbred embryos at two developmental stages (50%- and 90%-epiholy). Three thousand cDNA fragments were generated in four cDNA pools with 64 primer combinations. All differently displayed transcript-derived fragments (TDFs) were screened by (lot blot hybridization, and the selected sequences were further analyzed by semi-quantitative RT-PCR and quantitative real-time RT-PCR. Compared with ZR embryos, 12 genes were up-regulated and 12 were down-regulated in RZ embryos. The gene fragments were sequenced and subjected to BLASTN analysis. The sequences encoded various proteins which functioned at various levels of proliferation, growth, and development. One gene (ZR6), dramatically down-regulated in RZ embryos, was chosen for loss-of-function study; the knockdown of ZR6 gave rise to the phenotype resembling that of RZ embryos. (c) 2008 Elsevier Inc. All rights reserved.
关键词[WOS]: CELL NUCLEAR TRANSFER ; GENE-EXPRESSION ; ENUCLEATED EGGS ; XENOMITOCHONDRIAL CYBRIDS ; SACCHAROMYCES-CEREVISIAE ; GENOME ; MITOCHONDRIA ; DEFICIENCY ; STABILITY ; IMPACT
语种: 英语
WOS记录号: WOS:000261285000016
ISSN号: 0093-691X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7938
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China

Recommended Citation:
Liu, J.; Sun, Y. H.; Wang, Y. W.; Wang, N.; Pei, D. S.; Wang, Y. P.; Hu, W.; Zhu, Z. Y..Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP,THERIOGENOLOGY,2008,70(9):1525-1535
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