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学科主题: Developmental Biology
题名: Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development
作者: Xia, Jian-Hong1; Liu, Jing-Xia1; Zhou, Li1; Li, Zhi1; Gui, Jian-Fang1
通讯作者: Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: apolipoprotein ; apo-14 ; digestive system ; organogenesis ; gibel carp
刊名: INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
发表日期: 2008
DOI: 10.1387/ijdb.072519jx
卷: 52, 期:8, 页:1089-1098
收录类别: SCI
文章类型: Article
部门归属: [Xia, Jian-Hong; Liu, Jing-Xia; Zhou, Li; Li, Zhi; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]
类目[WOS]: Developmental Biology
研究领域[WOS]: Developmental Biology
摘要: Apo-14 is a fish-specific apolipoprotein and its biological function remains unknown. In this study, CagApo-14 was cloned from gibel carp (Carassius auratus gibelio) and its expression pattern was investigated during embryogenesis and early larval development. The CagApo-14 transcript and its protein product were firstly localized in the yolk syncytial layer at a high level during embryogenesis, and then found to be restricted to the digestive system including liver and intestine in later embryos and early larvae. Immunofluorescence staining in larvae and adults indicated that CagApo-14 protein was predominantly synthesized in and excreted from sinusoidal endothelial cells of liver tissue. Morpholino knockdown of CagApo-14 resulted in severe disruption of digestive organs including liver, intestine, pancreas and swim bladder. Moreover, yolk lipid transportation and utilization were severely affected in the CagApo-14 morphants. Overall, this data indicates that CagApo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.
英文摘要: Apo-14 is a fish-specific apolipoprotein and its biological function remains unknown. In this study, CagApo-14 was cloned from gibel carp (Carassius auratus gibelio) and its expression pattern was investigated during embryogenesis and early larval development. The CagApo-14 transcript and its protein product were firstly localized in the yolk syncytial layer at a high level during embryogenesis, and then found to be restricted to the digestive system including liver and intestine in later embryos and early larvae. Immunofluorescence staining in larvae and adults indicated that CagApo-14 protein was predominantly synthesized in and excreted from sinusoidal endothelial cells of liver tissue. Morpholino knockdown of CagApo-14 resulted in severe disruption of digestive organs including liver, intestine, pancreas and swim bladder. Moreover, yolk lipid transportation and utilization were severely affected in the CagApo-14 morphants. Overall, this data indicates that CagApo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.
关键词[WOS]: AURATUS-GIBELIO BLOCH ; SILVER CRUCIAN CARP ; APOLIPOPROTEIN-A-I ; GENE-EXPRESSION ; EMBRYONIC-DEVELOPMENT ; CARASSIUS-AURATUS ; LIVER DEVELOPMENT ; LIPID NUTRITION ; GUT DEVELOPMENT ; ZEBRAFISH
语种: 英语
WOS记录号: WOS:000262153300009
ISSN号: 0214-6282
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7926
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Xia, Jian-Hong; Liu, Jing-Xia; Zhou, Li; Li, Zhi; Gui, Jian-Fang.Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development,INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY,2008,52(8):1089-1098
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