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Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development
Xia, Jian-Hong; Liu, Jing-Xia; Zhou, Li; Li, Zhi; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008
Source PublicationINTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
ISSN0214-6282
Volume52Issue:8Pages:1089-1098
AbstractApo-14 is a fish-specific apolipoprotein and its biological function remains unknown. In this study, CagApo-14 was cloned from gibel carp (Carassius auratus gibelio) and its expression pattern was investigated during embryogenesis and early larval development. The CagApo-14 transcript and its protein product were firstly localized in the yolk syncytial layer at a high level during embryogenesis, and then found to be restricted to the digestive system including liver and intestine in later embryos and early larvae. Immunofluorescence staining in larvae and adults indicated that CagApo-14 protein was predominantly synthesized in and excreted from sinusoidal endothelial cells of liver tissue. Morpholino knockdown of CagApo-14 resulted in severe disruption of digestive organs including liver, intestine, pancreas and swim bladder. Moreover, yolk lipid transportation and utilization were severely affected in the CagApo-14 morphants. Overall, this data indicates that CagApo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.; Apo-14 is a fish-specific apolipoprotein and its biological function remains unknown. In this study, CagApo-14 was cloned from gibel carp (Carassius auratus gibelio) and its expression pattern was investigated during embryogenesis and early larval development. The CagApo-14 transcript and its protein product were firstly localized in the yolk syncytial layer at a high level during embryogenesis, and then found to be restricted to the digestive system including liver and intestine in later embryos and early larvae. Immunofluorescence staining in larvae and adults indicated that CagApo-14 protein was predominantly synthesized in and excreted from sinusoidal endothelial cells of liver tissue. Morpholino knockdown of CagApo-14 resulted in severe disruption of digestive organs including liver, intestine, pancreas and swim bladder. Moreover, yolk lipid transportation and utilization were severely affected in the CagApo-14 morphants. Overall, this data indicates that CagApo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.
SubtypeArticle
KeywordApolipoprotein Apo-14 Digestive System Organogenesis Gibel Carp
Department[Xia, Jian-Hong; Liu, Jing-Xia; Zhou, Li; Li, Zhi; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaDevelopmental Biology
DOI10.1387/ijdb.072519jx
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]
Indexed BySCI
Language英语
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000262153300009
WOS KeywordAURATUS-GIBELIO BLOCH ; SILVER CRUCIAN CARP ; APOLIPOPROTEIN-A-I ; GENE-EXPRESSION ; EMBRYONIC-DEVELOPMENT ; CARASSIUS-AURATUS ; LIVER DEVELOPMENT ; LIPID NUTRITION ; GUT DEVELOPMENT ; ZEBRAFISH
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]
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Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7926
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xia, Jian-Hong,Liu, Jing-Xia,Zhou, Li,et al. Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development[J]. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY,2008,52(8):1089-1098.
APA Xia, Jian-Hong,Liu, Jing-Xia,Zhou, Li,Li, Zhi,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY,52(8),1089-1098.
MLA Xia, Jian-Hong,et al."Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development".INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 52.8(2008):1089-1098.
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