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学科主题: Limnology; Marine & Freshwater Biology
题名: Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China
作者: Hu, Zhiquan1, 2; Li, Dunhai1; Xiao, Bo2; Dauta, Alain3; Liu, Yongding1
通讯作者: Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
关键词: Microcystin-RR ; Aphanizomenon sp DC01 ; physiological stress ; cell death ; ultrastructure
刊名: FUNDAMENTAL AND APPLIED LIMNOLOGY
发表日期: 2008
DOI: 10.1127/1863-9135/2008/0173-0111
卷: 173, 期:2, 页:111-120
收录类别: SCI
文章类型: Article
部门归属: [Hu, Zhiquan; Li, Dunhai; Liu, Yongding] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China; [Hu, Zhiquan; Xiao, Bo] Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Peoples R China; [Dauta, Alain] CNRS UPS INPT, UMR 5245, Equipe InterBio EcoLab, Lab Ecol Fonctionnelle, F-31055 Toulouse, France
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002]
类目[WOS]: Limnology ; Marine & Freshwater Biology
研究领域[WOS]: Marine & Freshwater Biology
摘要: The phytoplankton community in Lake Dianchi (Yunnan Province, Southwestern China) is dominated in April by a bloom of Aphanizomenon, that disappears Suddenly and is displaced by a Microcystis bloom in May. The reasons for the rapid bloom disappearance phenomenon and the temporal variability in the composition of phytoplankton assemblages are poorly understood. Cell growth, ultrastructure and physiological changes were examined in cultures of Aphanizomenon sp. DC01 isolated from Lake Dianchi exposed to different closes of rnicrocystin-RR (MC-RR) produced by the Microcystis bloom. MC-RR concentrations above 100 mu g L-1 markedly inhibited the pigment (chlorophyll-a, phycocyanin) synthesis and caused an increase of soluble carbohydrate and protein contents and nitrate reductase activity of toxin-treated blue-green algae. A drastic. reduction in photochemical efficiency of PSII (Fv/Fm) was also found. Morphological examinationn showed that the Aphanizomenon filaments disintegrated and file cells lysed gradually after 48 h Of toxin exposure. Transmission electron microscopy revealed that cellular inclusions of stressed cells almost leaked out completely and the cell membranes were grossly damaged. These findings demonstrate the allelopathic activity of Microcystis aeruginosa inducing physiological stress and cell death of Aphanizomenon sp. DC01 Although the active concentrations of microcystin were rather high, we propose that microcystin may function as allelopathic Substance due to inhomogeneous toxin concentrations close to Microcystis cells. Hence, it may play a role in species Succession of Aphanizomenon and Microcystis in Lake Dianchi.
英文摘要: The phytoplankton community in Lake Dianchi (Yunnan Province, Southwestern China) is dominated in April by a bloom of Aphanizomenon, that disappears Suddenly and is displaced by a Microcystis bloom in May. The reasons for the rapid bloom disappearance phenomenon and the temporal variability in the composition of phytoplankton assemblages are poorly understood. Cell growth, ultrastructure and physiological changes were examined in cultures of Aphanizomenon sp. DC01 isolated from Lake Dianchi exposed to different closes of rnicrocystin-RR (MC-RR) produced by the Microcystis bloom. MC-RR concentrations above 100 mu g L(-1) markedly inhibited the pigment (chlorophyll-a, phycocyanin) synthesis and caused an increase of soluble carbohydrate and protein contents and nitrate reductase activity of toxin-treated blue-green algae. A drastic. reduction in photochemical efficiency of PSII (Fv/Fm) was also found. Morphological examinationn showed that the Aphanizomenon filaments disintegrated and file cells lysed gradually after 48 h Of toxin exposure. Transmission electron microscopy revealed that cellular inclusions of stressed cells almost leaked out completely and the cell membranes were grossly damaged. These findings demonstrate the allelopathic activity of Microcystis aeruginosa inducing physiological stress and cell death of Aphanizomenon sp. DC01 Although the active concentrations of microcystin were rather high, we propose that microcystin may function as allelopathic Substance due to inhomogeneous toxin concentrations close to Microcystis cells. Hence, it may play a role in species Succession of Aphanizomenon and Microcystis in Lake Dianchi.
关键词[WOS]: DINOFLAGELLATE PERIDINIUM-GATUNENSE ; SYNECHOCOCCUS-ELONGATUS ; GROWTH ; PHOTOSYNTHESIS ; INHIBITION ; SUCCESSION ; TOXINS ; ALGA
语种: 英语
WOS记录号: WOS:000262356500003
ISSN号: 1863-9135
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7918
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
2.Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Peoples R China
3.CNRS UPS INPT, UMR 5245, Equipe InterBio EcoLab, Lab Ecol Fonctionnelle, F-31055 Toulouse, France

Recommended Citation:
Hu, Zhiquan; Li, Dunhai; Xiao, Bo; Dauta, Alain; Liu, Yongding.Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China,FUNDAMENTAL AND APPLIED LIMNOLOGY,2008,173(2):111-120
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