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Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China
Hu, Zhiquan1,2; Li, Dunhai1; Xiao, Bo2; Dauta, Alain3; Liu, Yongding1; Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
2008
Source PublicationFUNDAMENTAL AND APPLIED LIMNOLOGY
ISSN1863-9135
Volume173Issue:2Pages:111-120
AbstractThe phytoplankton community in Lake Dianchi (Yunnan Province, Southwestern China) is dominated in April by a bloom of Aphanizomenon, that disappears Suddenly and is displaced by a Microcystis bloom in May. The reasons for the rapid bloom disappearance phenomenon and the temporal variability in the composition of phytoplankton assemblages are poorly understood. Cell growth, ultrastructure and physiological changes were examined in cultures of Aphanizomenon sp. DC01 isolated from Lake Dianchi exposed to different closes of rnicrocystin-RR (MC-RR) produced by the Microcystis bloom. MC-RR concentrations above 100 mu g L-1 markedly inhibited the pigment (chlorophyll-a, phycocyanin) synthesis and caused an increase of soluble carbohydrate and protein contents and nitrate reductase activity of toxin-treated blue-green algae. A drastic. reduction in photochemical efficiency of PSII (Fv/Fm) was also found. Morphological examinationn showed that the Aphanizomenon filaments disintegrated and file cells lysed gradually after 48 h Of toxin exposure. Transmission electron microscopy revealed that cellular inclusions of stressed cells almost leaked out completely and the cell membranes were grossly damaged. These findings demonstrate the allelopathic activity of Microcystis aeruginosa inducing physiological stress and cell death of Aphanizomenon sp. DC01 Although the active concentrations of microcystin were rather high, we propose that microcystin may function as allelopathic Substance due to inhomogeneous toxin concentrations close to Microcystis cells. Hence, it may play a role in species Succession of Aphanizomenon and Microcystis in Lake Dianchi.; The phytoplankton community in Lake Dianchi (Yunnan Province, Southwestern China) is dominated in April by a bloom of Aphanizomenon, that disappears Suddenly and is displaced by a Microcystis bloom in May. The reasons for the rapid bloom disappearance phenomenon and the temporal variability in the composition of phytoplankton assemblages are poorly understood. Cell growth, ultrastructure and physiological changes were examined in cultures of Aphanizomenon sp. DC01 isolated from Lake Dianchi exposed to different closes of rnicrocystin-RR (MC-RR) produced by the Microcystis bloom. MC-RR concentrations above 100 mu g L(-1) markedly inhibited the pigment (chlorophyll-a, phycocyanin) synthesis and caused an increase of soluble carbohydrate and protein contents and nitrate reductase activity of toxin-treated blue-green algae. A drastic. reduction in photochemical efficiency of PSII (Fv/Fm) was also found. Morphological examinationn showed that the Aphanizomenon filaments disintegrated and file cells lysed gradually after 48 h Of toxin exposure. Transmission electron microscopy revealed that cellular inclusions of stressed cells almost leaked out completely and the cell membranes were grossly damaged. These findings demonstrate the allelopathic activity of Microcystis aeruginosa inducing physiological stress and cell death of Aphanizomenon sp. DC01 Although the active concentrations of microcystin were rather high, we propose that microcystin may function as allelopathic Substance due to inhomogeneous toxin concentrations close to Microcystis cells. Hence, it may play a role in species Succession of Aphanizomenon and Microcystis in Lake Dianchi.
SubtypeArticle
KeywordMicrocystin-rr Aphanizomenon Sp Dc01 Physiological Stress Cell Death Ultrastructure
Department[Hu, Zhiquan; Li, Dunhai; Liu, Yongding] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China; [Hu, Zhiquan; Xiao, Bo] Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Peoples R China; [Dauta, Alain] CNRS UPS INPT, UMR 5245, Equipe InterBio EcoLab, Lab Ecol Fonctionnelle, F-31055 Toulouse, France
Subject AreaLimnology ; Marine & Freshwater Biology
DOI10.1127/1863-9135/2008/0173-0111
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002] ; National Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002] ; National Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002] ; National Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectLimnology ; Marine & Freshwater Biology
WOS IDWOS:000262356500003
WOS KeywordDINOFLAGELLATE PERIDINIUM-GATUNENSE ; SYNECHOCOCCUS-ELONGATUS ; GROWTH ; PHOTOSYNTHESIS ; INHIBITION ; SUCCESSION ; TOXINS ; ALGA
Funding OrganizationNational Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002] ; National Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002] ; National Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002] ; National Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002]
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7918
Collection期刊论文
Corresponding AuthorLiu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
2.Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Peoples R China
3.CNRS UPS INPT, UMR 5245, Equipe InterBio EcoLab, Lab Ecol Fonctionnelle, F-31055 Toulouse, France
Recommended Citation
GB/T 7714
Hu, Zhiquan,Li, Dunhai,Xiao, Bo,et al. Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China[J]. FUNDAMENTAL AND APPLIED LIMNOLOGY,2008,173(2):111-120.
APA Hu, Zhiquan,Li, Dunhai,Xiao, Bo,Dauta, Alain,Liu, Yongding,&Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China.(2008).Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China.FUNDAMENTAL AND APPLIED LIMNOLOGY,173(2),111-120.
MLA Hu, Zhiquan,et al."Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China".FUNDAMENTAL AND APPLIED LIMNOLOGY 173.2(2008):111-120.
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