中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
学科主题: Immunology; Microbiology
题名: Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2
作者: Li, Qiong1, 2; Kumar, Ashok1; Gui, Jian-Fang2; Yu, Fu-Shin X.1
通讯作者: Yu, FSX, Wayne State Univ, Sch Med, Dept Ophthalmol, Kresge Eye Inst, Detroit, MI 48201 USA
关键词: Staphylococcus aureus ; Toll-like receptors ; Innate resposne ; Lipoprotein ; Corneal epithelium
刊名: MICROBIAL PATHOGENESIS
发表日期: 2008-05-01
DOI: 10.1016/j.micpath.2007.11.006
卷: 44, 期:5, 页:426-434
收录类别: SCI
文章类型: Article
部门归属: [Li, Qiong; Kumar, Ashok; Yu, Fu-Shin X.] Wayne State Univ, Sch Med, Dept Ophthalmol, Kresge Eye Inst, Detroit, MI 48201 USA; [Li, Qiong; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: NIH [R01EY14080, EY10869]; Research to Prevent Blindness to the Department of Ophthalmology, Wayne State University School of Medicine
类目[WOS]: Immunology ; Microbiology
研究领域[WOS]: Immunology ; Microbiology
摘要: Bacterial lipoproteins (LP) are a family of cell wall components found in a wide variety of bacteria. In this study, we characterized the response of HUCL, a telomerase-immortalized human corneal epithelial cell (HCEC) line, to LP isolated from Staphylococcus (S) aureus. S. aureus LP (saLP) prepared by Triton X-114 extraction stimulated the activation of NF-kappa B, JNK, and P38 signaling pathways in HUCL cells. The extracts failed to stimulate NF-kappa B activation in HUCL cells after lipoprotein lipase treatment and in cell lines expressing TLR4 or TLR9, but not TLR2, indicating lipoprotein nature of the extracts. saLP induced the up-regulation of a variety of inflammatory cytokines and chemokines (IL-6, IL-8, ICAM-1). antimicrobial molecules (hBD-2, LL-37, and iNOS), and homeostasis genes (Mn-SOD) at both the mRNA level and protein level. Similar inflammatory response to saLP was also observed in primarily cultured HCECs using the production of IL-6 as readout. Moreover, TLR2 neutralizing antibody blocked the saLP-induced secretion of IL-6, IL-8 and hBD2 in HUCL cells. Our findings suggest that saLP activates TLR2 and triggers innate immune response in the cornea to S. aureus infection via production of proinflammatory cytokines and defense molecules. (C) 2007 Elsevier Ltd. All rights reserved.
英文摘要: Bacterial lipoproteins (LP) are a family of cell wall components found in a wide variety of bacteria. In this study, we characterized the response of HUCL, a telomerase-immortalized human corneal epithelial cell (HCEC) line, to LP isolated from Staphylococcus (S) aureus. S. aureus LP (saLP) prepared by Triton X-114 extraction stimulated the activation of NF-kappa B, JNK, and P38 signaling pathways in HUCL cells. The extracts failed to stimulate NF-kappa B activation in HUCL cells after lipoprotein lipase treatment and in cell lines expressing TLR4 or TLR9, but not TLR2, indicating lipoprotein nature of the extracts. saLP induced the up-regulation of a variety of inflammatory cytokines and chemokines (IL-6, IL-8, ICAM-1). antimicrobial molecules (hBD-2, LL-37, and iNOS), and homeostasis genes (Mn-SOD) at both the mRNA level and protein level. Similar inflammatory response to saLP was also observed in primarily cultured HCECs using the production of IL-6 as readout. Moreover, TLR2 neutralizing antibody blocked the saLP-induced secretion of IL-6, IL-8 and hBD2 in HUCL cells. Our findings suggest that saLP activates TLR2 and triggers innate immune response in the cornea to S. aureus infection via production of proinflammatory cytokines and defense molecules. (C) 2007 Elsevier Ltd. All rights reserved.
关键词[WOS]: CELL-WALL COMPONENTS ; BACTERIAL KERATITIS ; HELICOBACTER-PYLORI ; LIPOTEICHOIC ACID ; SHIFTING TRENDS ; CUTTING EDGE ; PROTEIN-A ; PEPTIDOGLYCAN ; EXPRESSION ; INFECTION
语种: 英语
WOS记录号: WOS:000262591100008
ISSN号: 0882-4010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7906
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2.pdf(543KB)----开放获取--View Download

作者单位: 1.Wayne State Univ, Sch Med, Dept Ophthalmol, Kresge Eye Inst, Detroit, MI 48201 USA
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Li, Qiong; Kumar, Ashok; Gui, Jian-Fang; Yu, Fu-Shin X..Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2,MICROBIAL PATHOGENESIS,2008,44(5):426-434
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Qiong]'s Articles
[Kumar, Ashok]'s Articles
[Gui, Jian-Fang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Qiong]‘s Articles
[Kumar, Ashok]‘s Articles
[Gui, Jian-Fang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace