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Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla)
Feng Jianjun1,2; Guan Ruizhang1; Lin Peng; Guo Songlin1,2; Guan, RZ, Jimei Univ, Coll Fisheries, 183 Yin Jiang Rd, Jimei 361021, Peoples R China
2009-01-15
Source PublicationVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN0165-2427
Volume127Issue:1-2Pages:144-147
AbstractIn this study, the immunoglobulin M heavy chain gene of European eel (Anguilla anguilla) was cloned and analyzed. The full-length cDNA of the IgM heavy chain gene (GenBank accession no. EF062515) has 2089 nucleotides encoding a putative protein of 581 amino acids. The IgM heavy chain was composed of leader peptide (L), variable domain (VH), CH1, CH2. Hinge, CH3, CH4, and C-terminus and two novel continuous putative N-glycosylation sites were found close to the second cysteine of CH3 in A. anguilla-H1 and A. anguilla-H2. The deduced amino acid sequence of the European eel IgM heavy chain constant region shared similarities to that of the Ladyfish (Elops saurus). Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), Grass carp (Ctenopharingodon idella), Common carp (Cyprinus carpio), Channel catfish (Ictalurus punctatus), and the orange-spotted grouper (Epinephelus coioides) with the identity of 46.1%, 39.7%, 38.9%, 32.4%, 32.3%, 31.7%, and 30.7%, respectively. The highest level of IgM gene expression was observed in the kidney, followed by the spleen, gills, liver, muscle and heart in the apparently healthy European eels. (C) 2008 Elsevier B.V. All rights reserved.; In this study, the immunoglobulin M heavy chain gene of European eel (Anguilla anguilla) was cloned and analyzed. The full-length cDNA of the IgM heavy chain gene (GenBank accession no. EF062515) has 2089 nucleotides encoding a putative protein of 581 amino acids. The IgM heavy chain was composed of leader peptide (L), variable domain (VH), CH1, CH2. Hinge, CH3, CH4, and C-terminus and two novel continuous putative N-glycosylation sites were found close to the second cysteine of CH3 in A. anguilla-H1 and A. anguilla-H2. The deduced amino acid sequence of the European eel IgM heavy chain constant region shared similarities to that of the Ladyfish (Elops saurus). Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), Grass carp (Ctenopharingodon idella), Common carp (Cyprinus carpio), Channel catfish (Ictalurus punctatus), and the orange-spotted grouper (Epinephelus coioides) with the identity of 46.1%, 39.7%, 38.9%, 32.4%, 32.3%, 31.7%, and 30.7%, respectively. The highest level of IgM gene expression was observed in the kidney, followed by the spleen, gills, liver, muscle and heart in the apparently healthy European eels. (C) 2008 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordCloning Heavy Chain Gene Immunoglobulin m European Eel Real-time Pcr
Department[Feng Jianjun; Guan Ruizhang; Guo Songlin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Feng Jianjun; Guo Songlin] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaImmunology ; Veterinary Sciences
DOI10.1016/j.vetimm.2008.09.022
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationAgriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203] ; Agriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203] ; Agriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203] ; Agriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Veterinary Sciences
WOS SubjectImmunology ; Veterinary Sciences
WOS IDWOS:000262781300015
WOS KeywordTELEOST FISH ; DISCOVERY ; ISOTYPE
Funding OrganizationAgriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203] ; Agriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203] ; Agriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203] ; Agriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203]
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7898
Collection期刊论文
Corresponding AuthorGuan, RZ, Jimei Univ, Coll Fisheries, 183 Yin Jiang Rd, Jimei 361021, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Feng Jianjun,Guan Ruizhang,Lin Peng,et al. Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla)[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2009,127(1-2):144-147.
APA Feng Jianjun,Guan Ruizhang,Lin Peng,Guo Songlin,&Guan, RZ, Jimei Univ, Coll Fisheries, 183 Yin Jiang Rd, Jimei 361021, Peoples R China.(2009).Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla).VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,127(1-2),144-147.
MLA Feng Jianjun,et al."Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla)".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 127.1-2(2009):144-147.
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