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Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus)
Ma, Liwen1,2; Li, Dongliang1,2; Wang, Jianwei1; He, Jiangyan1; Yin, Zhan1,2; Yin, Z, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu S St, Wuhan 430072, Peoples R China
2009-01-18
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume91Issue:1Pages:19-25
AbstractTeleost vitellogenins (VTGs) are large multidomain apolipoproteins and traditionally considered as the estrogen responsive precursors of the major egg yolk proteins. We identified five clones encoding VTGs, about 16% of the random EST clones from our constructed cDNA library from Chinese rare minnow liver tissue treated with 17 beta-estradiol (E2). Full-length vtgAo1 has been obtained based on the sequence information of four partial cDNA inserts by RACE. The inducibility of the vtgAo1 expression in liver by E2 was confirmed by RT-PCR. The presence of vtgAo1 transcripts have been observed primarily in liver. However. a significant level of the vtgAo1 was found in an unexpected location, heart, particularly in atrial cells by RT-PCR and whole mount in situ hybridization analyses. The vtgAo1 mRNA expression in heart and liver tissue could be suppressed by both alpha-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). The expression of VTG in the heart observed in the present studies suggested it may provide protection from surplus intracellular lipids in fish cardiomyocytes as triglyceride transport proteins do in mammals. The results also indicated that the production of teleost vtg in vivo can be regulated by riot only estrogenic agents, but adrenergic signals as well. (c) 2008 Elsevier B.V. All rights reserved.; Teleost vitellogenins (VTGs) are large multidomain apolipoproteins and traditionally considered as the estrogen responsive precursors of the major egg yolk proteins. We identified five clones encoding VTGs, about 16% of the random EST clones from our constructed cDNA library from Chinese rare minnow liver tissue treated with 17 beta-estradiol (E2). Full-length vtgAo1 has been obtained based on the sequence information of four partial cDNA inserts by RACE. The inducibility of the vtgAo1 expression in liver by E2 was confirmed by RT-PCR. The presence of vtgAo1 transcripts have been observed primarily in liver. However. a significant level of the vtgAo1 was found in an unexpected location, heart, particularly in atrial cells by RT-PCR and whole mount in situ hybridization analyses. The vtgAo1 mRNA expression in heart and liver tissue could be suppressed by both alpha-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). The expression of VTG in the heart observed in the present studies suggested it may provide protection from surplus intracellular lipids in fish cardiomyocytes as triglyceride transport proteins do in mammals. The results also indicated that the production of teleost vtg in vivo can be regulated by riot only estrogenic agents, but adrenergic signals as well. (c) 2008 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordChinese Rare Minnow Vtgao1 Adrenergic Agonist Heart Liver
Department[Ma, Liwen; Li, Dongliang; Wang, Jianwei; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Ma, Liwen; Li, Dongliang; Yin, Zhan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Subject AreaMarine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2008.09.015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationChina Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000263021100003
WOS KeywordZEBRAFISH DANIO-RERIO ; APOLIPOPROTEIN-B ; ENDOCRINE DISRUPTION ; PIMEPHALES-PROMELAS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; TRIGLYCERIDE ; RESPONSES ; EXPOSURE ; LIVER
Funding OrganizationChina Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7892
Collection期刊论文
Corresponding AuthorYin, Z, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu S St, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Ma, Liwen,Li, Dongliang,Wang, Jianwei,et al. Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus)[J]. AQUATIC TOXICOLOGY,2009,91(1):19-25.
APA Ma, Liwen,Li, Dongliang,Wang, Jianwei,He, Jiangyan,Yin, Zhan,&Yin, Z, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu S St, Wuhan 430072, Peoples R China.(2009).Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus).AQUATIC TOXICOLOGY,91(1),19-25.
MLA Ma, Liwen,et al."Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus)".AQUATIC TOXICOLOGY 91.1(2009):19-25.
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