| Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus) | |
| Ma, Liwen1,2; Li, Dongliang1,2; Wang, Jianwei1; He, Jiangyan1; Yin, Zhan1,2; Yin, Z, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu S St, Wuhan 430072, Peoples R China | |
| 2009-01-18 | |
| Source Publication | AQUATIC TOXICOLOGY
 |
| ISSN | 0166-445X |
| Volume | 91Issue:1Pages:19-25 |
| Abstract | Teleost vitellogenins (VTGs) are large multidomain apolipoproteins and traditionally considered as the estrogen responsive precursors of the major egg yolk proteins. We identified five clones encoding VTGs, about 16% of the random EST clones from our constructed cDNA library from Chinese rare minnow liver tissue treated with 17 beta-estradiol (E2). Full-length vtgAo1 has been obtained based on the sequence information of four partial cDNA inserts by RACE. The inducibility of the vtgAo1 expression in liver by E2 was confirmed by RT-PCR. The presence of vtgAo1 transcripts have been observed primarily in liver. However. a significant level of the vtgAo1 was found in an unexpected location, heart, particularly in atrial cells by RT-PCR and whole mount in situ hybridization analyses. The vtgAo1 mRNA expression in heart and liver tissue could be suppressed by both alpha-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). The expression of VTG in the heart observed in the present studies suggested it may provide protection from surplus intracellular lipids in fish cardiomyocytes as triglyceride transport proteins do in mammals. The results also indicated that the production of teleost vtg in vivo can be regulated by riot only estrogenic agents, but adrenergic signals as well. (c) 2008 Elsevier B.V. All rights reserved.; Teleost vitellogenins (VTGs) are large multidomain apolipoproteins and traditionally considered as the estrogen responsive precursors of the major egg yolk proteins. We identified five clones encoding VTGs, about 16% of the random EST clones from our constructed cDNA library from Chinese rare minnow liver tissue treated with 17 beta-estradiol (E2). Full-length vtgAo1 has been obtained based on the sequence information of four partial cDNA inserts by RACE. The inducibility of the vtgAo1 expression in liver by E2 was confirmed by RT-PCR. The presence of vtgAo1 transcripts have been observed primarily in liver. However. a significant level of the vtgAo1 was found in an unexpected location, heart, particularly in atrial cells by RT-PCR and whole mount in situ hybridization analyses. The vtgAo1 mRNA expression in heart and liver tissue could be suppressed by both alpha-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). The expression of VTG in the heart observed in the present studies suggested it may provide protection from surplus intracellular lipids in fish cardiomyocytes as triglyceride transport proteins do in mammals. The results also indicated that the production of teleost vtg in vivo can be regulated by riot only estrogenic agents, but adrenergic signals as well. (c) 2008 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Chinese Rare Minnow Vtgao1 Adrenergic Agonist Heart Liver |
| Department | [Ma, Liwen; Li, Dongliang; Wang, Jianwei; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Ma, Liwen; Li, Dongliang; Yin, Zhan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| Subject Area | Marine & Freshwater Biology ; Toxicology |
| DOI | 10.1016/j.aquatox.2008.09.015 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Marine & Freshwater Biology ; Toxicology |
| WOS Subject | Marine & Freshwater Biology ; Toxicology |
| WOS ID | WOS:000263021100003 |
| WOS Keyword | ZEBRAFISH DANIO-RERIO ; APOLIPOPROTEIN-B ; ENDOCRINE DISRUPTION ; PIMEPHALES-PROMELAS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; TRIGLYCERIDE ; RESPONSES ; EXPOSURE ; LIVER |
| Funding Organization | China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] ; China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7892 |
| Collection | 期刊论文 |
| Corresponding Author | Yin, Z, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu S St, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Ma, Liwen,Li, Dongliang,Wang, Jianwei,et al. Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus)[J]. AQUATIC TOXICOLOGY,2009,91(1):19-25. |
| APA | Ma, Liwen,Li, Dongliang,Wang, Jianwei,He, Jiangyan,Yin, Zhan,&Yin, Z, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu S St, Wuhan 430072, Peoples R China.(2009).Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus).AQUATIC TOXICOLOGY,91(1),19-25. |
| MLA | Ma, Liwen,et al."Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus)".AQUATIC TOXICOLOGY 91.1(2009):19-25. |
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